Structural characterization and identification of cyclic lipopeptides produced by Bacillus methylotrophicus DCS1 strain

Bacillus methylotrophicus DCS1 strain was isolated from diesel contaminated soil and screened for its ability to produce biosurfactants; it was found effective for the production of surface active molecules. The structural characterization of the isolated lipopeptides was studied by a variety of ana...

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Autores: Jemil, Nawel, Manresa Presas, Ma. Ángeles (María Ángeles), Rabanal Anglada, Francesc, Ben Ayed, Hanen, Hmidet, Noomen, Nasri, Moncef
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/184959
Acceso en línea:https://hdl.handle.net/2445/184959
Access Level:acceso abierto
Palabra clave:Bacils
Pèptids
Estructura molecular
Biotecnologia
Bacillus (Bacteria)
Peptides
Molecular structure
Biotechnology
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spelling Structural characterization and identification of cyclic lipopeptides produced by Bacillus methylotrophicus DCS1 strainJemil, NawelManresa Presas, Ma. Ángeles (María Ángeles)Rabanal Anglada, FrancescBen Ayed, HanenHmidet, NoomenNasri, MoncefBacilsPèptidsEstructura molecularBiotecnologiaBacillus (Bacteria)PeptidesMolecular structureBiotechnologyBacillus methylotrophicus DCS1 strain was isolated from diesel contaminated soil and screened for its ability to produce biosurfactants; it was found effective for the production of surface active molecules. The structural characterization of the isolated lipopeptides was studied by a variety of analytical techniques. The organic extract of DCS1 'lipopeptides was fractionated by silica gel column chromatography (60 Mesh). Fractions containing lipopeptides were collected and identified by tandem mass spectrometry MALDI-TOF-MS and MALDI-TOF MS2. The crude biosurfactants contains a mixture of homologous lipopeptides with molecular weights between 1016 and 1556 Da. Mass spectrometry analysis of partially purified lipopeptides revealed that it contains different isoforms belonging to three families: surfactin, iturin and fengycin. To identify lipopeptides isoforms, MALDI-TOF MS2 was used and ions representing characteristic fragmentations were detected. The mass spectrometry characterization revealed the presence of four variants of surfactin lipopeptides, four variants of pumilacidin that differ according to the-beta-hydroxy fatty acid chain length as well as the type of amino acid at position 7, five variants of iturin A/mycosubtilin varying in the beta-amino fatty acid chain length from C12 to C16, C16 iturin Cl, five isoforms of bacillomycin D varying in the beta-amino fatty acid chain length from C14 to C18, and six fengycin isoforms that differ according to the length of the beta-hydroxy fatty acid side chain as well as the amino acid at position 6. The capacity of B. methylotrohicus DCS1 strain to produce many lipopeptides isoforms belonging to different families and having a structural diversity is a very interesting characteristic that allows them to be used in various fields of biotechnological applications.Elsevier B.V.2022202220172022info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion13 p.application/pdfhttps://hdl.handle.net/2445/184959Articles publicats en revistes (Química Inorgànica i Orgànica)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1016/j.jchromb.2017.06.013Journal Of Chromatography B-Analytical Technologies In The Biomedical And Life Sciences, 2017, vol. 1060, p. 374-386https://doi.org/10.1016/j.jchromb.2017.06.013cc-by-nc-nd (c) Elsevier B.V., 2017https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1849592026-05-29T05:05:01Z
dc.title.none.fl_str_mv Structural characterization and identification of cyclic lipopeptides produced by Bacillus methylotrophicus DCS1 strain
title Structural characterization and identification of cyclic lipopeptides produced by Bacillus methylotrophicus DCS1 strain
spellingShingle Structural characterization and identification of cyclic lipopeptides produced by Bacillus methylotrophicus DCS1 strain
Jemil, Nawel
Bacils
Pèptids
Estructura molecular
Biotecnologia
Bacillus (Bacteria)
Peptides
Molecular structure
Biotechnology
title_short Structural characterization and identification of cyclic lipopeptides produced by Bacillus methylotrophicus DCS1 strain
title_full Structural characterization and identification of cyclic lipopeptides produced by Bacillus methylotrophicus DCS1 strain
title_fullStr Structural characterization and identification of cyclic lipopeptides produced by Bacillus methylotrophicus DCS1 strain
title_full_unstemmed Structural characterization and identification of cyclic lipopeptides produced by Bacillus methylotrophicus DCS1 strain
title_sort Structural characterization and identification of cyclic lipopeptides produced by Bacillus methylotrophicus DCS1 strain
dc.creator.none.fl_str_mv Jemil, Nawel
Manresa Presas, Ma. Ángeles (María Ángeles)
Rabanal Anglada, Francesc
Ben Ayed, Hanen
Hmidet, Noomen
Nasri, Moncef
author Jemil, Nawel
author_facet Jemil, Nawel
Manresa Presas, Ma. Ángeles (María Ángeles)
Rabanal Anglada, Francesc
Ben Ayed, Hanen
Hmidet, Noomen
Nasri, Moncef
author_role author
author2 Manresa Presas, Ma. Ángeles (María Ángeles)
Rabanal Anglada, Francesc
Ben Ayed, Hanen
Hmidet, Noomen
Nasri, Moncef
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bacils
Pèptids
Estructura molecular
Biotecnologia
Bacillus (Bacteria)
Peptides
Molecular structure
Biotechnology
topic Bacils
Pèptids
Estructura molecular
Biotecnologia
Bacillus (Bacteria)
Peptides
Molecular structure
Biotechnology
description Bacillus methylotrophicus DCS1 strain was isolated from diesel contaminated soil and screened for its ability to produce biosurfactants; it was found effective for the production of surface active molecules. The structural characterization of the isolated lipopeptides was studied by a variety of analytical techniques. The organic extract of DCS1 'lipopeptides was fractionated by silica gel column chromatography (60 Mesh). Fractions containing lipopeptides were collected and identified by tandem mass spectrometry MALDI-TOF-MS and MALDI-TOF MS2. The crude biosurfactants contains a mixture of homologous lipopeptides with molecular weights between 1016 and 1556 Da. Mass spectrometry analysis of partially purified lipopeptides revealed that it contains different isoforms belonging to three families: surfactin, iturin and fengycin. To identify lipopeptides isoforms, MALDI-TOF MS2 was used and ions representing characteristic fragmentations were detected. The mass spectrometry characterization revealed the presence of four variants of surfactin lipopeptides, four variants of pumilacidin that differ according to the-beta-hydroxy fatty acid chain length as well as the type of amino acid at position 7, five variants of iturin A/mycosubtilin varying in the beta-amino fatty acid chain length from C12 to C16, C16 iturin Cl, five isoforms of bacillomycin D varying in the beta-amino fatty acid chain length from C14 to C18, and six fengycin isoforms that differ according to the length of the beta-hydroxy fatty acid side chain as well as the amino acid at position 6. The capacity of B. methylotrohicus DCS1 strain to produce many lipopeptides isoforms belonging to different families and having a structural diversity is a very interesting characteristic that allows them to be used in various fields of biotechnological applications.
publishDate 2017
dc.date.none.fl_str_mv 2017
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/184959
url https://hdl.handle.net/2445/184959
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/j.jchromb.2017.06.013
Journal Of Chromatography B-Analytical Technologies In The Biomedical And Life Sciences, 2017, vol. 1060, p. 374-386
https://doi.org/10.1016/j.jchromb.2017.06.013
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Elsevier B.V., 2017
https://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Elsevier B.V., 2017
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 13 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Química Inorgànica i Orgànica)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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