Amperometric Biosensor for Continuous Monitoring Irgarol 1051 in Sea Water
Irgarol 1051 is one of the most employed antifouling agents and is one of the most commonly found in marine environment. According to its widespread use the European Comission turned its attention on this agent as one of the priority substances that has to be controlled. The aim of this article is t...
| Autores: | , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2016 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/376751 |
| Acceso en línea: | http://hdl.handle.net/10261/376751 https://api.elsevier.com/content/abstract/scopus_id/84971483958 |
| Access Level: | acceso abierto |
| Palabra clave: | Sea-water Amperometric Biosensor Irgarol http://metadata.un.org/sdg/6 http://metadata.un.org/sdg/9 http://metadata.un.org/sdg/3 Ensure healthy lives and promote well-being for all at all ages Ensure availability and sustainable management of water and sanitation for all Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation Ensure sustainable consumption and production patterns |
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Amperometric Biosensor for Continuous Monitoring Irgarol 1051 in Sea WaterSalvador, Juan PabloMarco, María PilarSea-waterAmperometricBiosensorIrgarolhttp://metadata.un.org/sdg/6http://metadata.un.org/sdg/9http://metadata.un.org/sdg/3Ensure healthy lives and promote well-being for all at all agesEnsure availability and sustainable management of water and sanitation for allBuild resilient infrastructure, promote inclusive and sustainable industrialization and foster innovationEnsure sustainable consumption and production patternsIrgarol 1051 is one of the most employed antifouling agents and is one of the most commonly found in marine environment. According to its widespread use the European Comission turned its attention on this agent as one of the priority substances that has to be controlled. The aim of this article is to develop an immunosensor for the determination of Irgarol 1051 in sea water for implementation in a flow analysis system. The transducing principle employed for its development was an amperometric detection using gold screen printed electrodes which were functionalized for the covalent immobilization of the immunoreagents. The detection of Irgarol 1051 was performed using specific antibodies working under competitive indirect format. The immunosensor was demonstrated to measure directly in sea water with high detectability, accurately and robust. The system allows consecutive measurements using the same chip owing to an easy regeneration process. The limit of detection reached was 0.15±0.09 nM (0.038±0.022 µg ⋅ L−1) measured directly in sea water.This work has been funded by SEA-on-a-chip project (FP7-OCEAN-2013-614168). The Nb4D group (formerly Applied Molecular Receptors group, AMRg) is a consolidated research group (Grup de Recerca) of the Generalitat de Catalunya and has support from the Departament d'Universitats, Recerca i Societat de la Informació de la Generalitat de Catalunya (expedient: 2014 SGR 1484). CIBER-BBN is an initiative funded by the Spanish National Plan for Scientific and Technical Research and Innovation 2013–2016, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions are financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. The ICTS “NANOBIOSIS”, and particularly the Custom Antibody Service (CAbS, IQAC-CSIC, CIBER-BBN), is acknowledged for the assistance and support related to the immunoreagents used in this work.Peer reviewedJohn Wiley & SonsEuropean CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/376751https://api.elsevier.com/content/abstract/scopus_id/84971483958reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/FP7/614168Electroanalysishttps://doi.org/10.1002/elan.201600172Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3767512026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
Amperometric Biosensor for Continuous Monitoring Irgarol 1051 in Sea Water |
| title |
Amperometric Biosensor for Continuous Monitoring Irgarol 1051 in Sea Water |
| spellingShingle |
Amperometric Biosensor for Continuous Monitoring Irgarol 1051 in Sea Water Salvador, Juan Pablo Sea-water Amperometric Biosensor Irgarol http://metadata.un.org/sdg/6 http://metadata.un.org/sdg/9 http://metadata.un.org/sdg/3 Ensure healthy lives and promote well-being for all at all ages Ensure availability and sustainable management of water and sanitation for all Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation Ensure sustainable consumption and production patterns |
| title_short |
Amperometric Biosensor for Continuous Monitoring Irgarol 1051 in Sea Water |
| title_full |
Amperometric Biosensor for Continuous Monitoring Irgarol 1051 in Sea Water |
| title_fullStr |
Amperometric Biosensor for Continuous Monitoring Irgarol 1051 in Sea Water |
| title_full_unstemmed |
Amperometric Biosensor for Continuous Monitoring Irgarol 1051 in Sea Water |
| title_sort |
Amperometric Biosensor for Continuous Monitoring Irgarol 1051 in Sea Water |
| dc.creator.none.fl_str_mv |
Salvador, Juan Pablo Marco, María Pilar |
| author |
Salvador, Juan Pablo |
| author_facet |
Salvador, Juan Pablo Marco, María Pilar |
| author_role |
author |
| author2 |
Marco, María Pilar |
| author2_role |
author |
| dc.contributor.none.fl_str_mv |
European Commission Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Sea-water Amperometric Biosensor Irgarol http://metadata.un.org/sdg/6 http://metadata.un.org/sdg/9 http://metadata.un.org/sdg/3 Ensure healthy lives and promote well-being for all at all ages Ensure availability and sustainable management of water and sanitation for all Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation Ensure sustainable consumption and production patterns |
| topic |
Sea-water Amperometric Biosensor Irgarol http://metadata.un.org/sdg/6 http://metadata.un.org/sdg/9 http://metadata.un.org/sdg/3 Ensure healthy lives and promote well-being for all at all ages Ensure availability and sustainable management of water and sanitation for all Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation Ensure sustainable consumption and production patterns |
| description |
Irgarol 1051 is one of the most employed antifouling agents and is one of the most commonly found in marine environment. According to its widespread use the European Comission turned its attention on this agent as one of the priority substances that has to be controlled. The aim of this article is to develop an immunosensor for the determination of Irgarol 1051 in sea water for implementation in a flow analysis system. The transducing principle employed for its development was an amperometric detection using gold screen printed electrodes which were functionalized for the covalent immobilization of the immunoreagents. The detection of Irgarol 1051 was performed using specific antibodies working under competitive indirect format. The immunosensor was demonstrated to measure directly in sea water with high detectability, accurately and robust. The system allows consecutive measurements using the same chip owing to an easy regeneration process. The limit of detection reached was 0.15±0.09 nM (0.038±0.022 µg ⋅ L−1) measured directly in sea water. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016 2025 2025 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Postprint info:eu-repo/semantics/acceptedVersion |
| format |
article |
| status_str |
acceptedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/376751 https://api.elsevier.com/content/abstract/scopus_id/84971483958 |
| url |
http://hdl.handle.net/10261/376751 https://api.elsevier.com/content/abstract/scopus_id/84971483958 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/FP7/614168 Electroanalysis https://doi.org/10.1002/elan.201600172 Sí |
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info:eu-repo/semantics/openAccess |
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openAccess |
| dc.publisher.none.fl_str_mv |
John Wiley & Sons |
| publisher.none.fl_str_mv |
John Wiley & Sons |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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1869406979575775232 |
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15.81155 |