Lineage Relationships Between Subpallial Progenitors and Glial Cells in the Piriform Cortex

The piriform cortex is a paleocortical area, located in the ventrolateral surface of the rodent forebrain, receiving direct input from the olfactory bulb. The three layers of the PC are defined by the diversity of glial and neuronal cells, marker expression, connections, and functions. However, the...

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Detalles Bibliográficos
Autores: Sánchez-González, Rebeca, López-Mascaraque, Laura
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/278450
Acceso en línea:http://hdl.handle.net/10261/278450
Access Level:acceso abierto
Palabra clave:NG2-glia
Astrocytes
clonal analysis
glial cells
heterogeneity
lineage
Olfactory system
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spelling Lineage Relationships Between Subpallial Progenitors and Glial Cells in the Piriform CortexSánchez-González, RebecaLópez-Mascaraque, LauraNG2-gliaAstrocytesclonal analysisglial cellsheterogeneitylineageOlfactory systemThe piriform cortex is a paleocortical area, located in the ventrolateral surface of the rodent forebrain, receiving direct input from the olfactory bulb. The three layers of the PC are defined by the diversity of glial and neuronal cells, marker expression, connections, and functions. However, the glial layering, ontogeny, and sibling cell relationship along the PC is an unresolved question in the field. Here, using multi-color genetic lineage tracing approaches with different StarTrack strategies, we performed a rigorous analysis of the derived cell progenies from progenitors located at the subpallium ventricular surface. First, we specifically targeted E12-progenitors with UbC-StarTrack to analyze their adult derived-cell progeny and their location within the piriform cortex layers. The vast majority of the cell progeny derived from targeted progenitors were identified as neurons, but also astrocytes and NG2 cells. Further, to specifically target single Gsx-2 subpallial progenitors and their derived cell-progeny in the piriform cortex, we used the UbC-(Gsx-2-hyPB)-StarTrack to perform an accurate analysis of their clonal relationships. Our results quantitatively delineate the adult clonal cell pattern from single subpallial E12-progenitors, focusing on glial cells. In summary, there is a temporal pattern in the assembly of the glial cell diversity in the piriform cortex, which also reveals spatio-temporal progenitor heterogeneity.This work was supported by the Spanish Research Grant PID2019-105218RB-I00 funded by MCIN/AEI/10.13039/501100 01103.Frontiers in Bioscience PublicationsMinisterio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/278450reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105218RB-I00http://dx.doi.org/10.3389/fnins.2022.825969Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2784502026-05-22T06:33:51Z
dc.title.none.fl_str_mv Lineage Relationships Between Subpallial Progenitors and Glial Cells in the Piriform Cortex
title Lineage Relationships Between Subpallial Progenitors and Glial Cells in the Piriform Cortex
spellingShingle Lineage Relationships Between Subpallial Progenitors and Glial Cells in the Piriform Cortex
Sánchez-González, Rebeca
NG2-glia
Astrocytes
clonal analysis
glial cells
heterogeneity
lineage
Olfactory system
title_short Lineage Relationships Between Subpallial Progenitors and Glial Cells in the Piriform Cortex
title_full Lineage Relationships Between Subpallial Progenitors and Glial Cells in the Piriform Cortex
title_fullStr Lineage Relationships Between Subpallial Progenitors and Glial Cells in the Piriform Cortex
title_full_unstemmed Lineage Relationships Between Subpallial Progenitors and Glial Cells in the Piriform Cortex
title_sort Lineage Relationships Between Subpallial Progenitors and Glial Cells in the Piriform Cortex
dc.creator.none.fl_str_mv Sánchez-González, Rebeca
López-Mascaraque, Laura
author Sánchez-González, Rebeca
author_facet Sánchez-González, Rebeca
López-Mascaraque, Laura
author_role author
author2 López-Mascaraque, Laura
author2_role author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv NG2-glia
Astrocytes
clonal analysis
glial cells
heterogeneity
lineage
Olfactory system
topic NG2-glia
Astrocytes
clonal analysis
glial cells
heterogeneity
lineage
Olfactory system
description The piriform cortex is a paleocortical area, located in the ventrolateral surface of the rodent forebrain, receiving direct input from the olfactory bulb. The three layers of the PC are defined by the diversity of glial and neuronal cells, marker expression, connections, and functions. However, the glial layering, ontogeny, and sibling cell relationship along the PC is an unresolved question in the field. Here, using multi-color genetic lineage tracing approaches with different StarTrack strategies, we performed a rigorous analysis of the derived cell progenies from progenitors located at the subpallium ventricular surface. First, we specifically targeted E12-progenitors with UbC-StarTrack to analyze their adult derived-cell progeny and their location within the piriform cortex layers. The vast majority of the cell progeny derived from targeted progenitors were identified as neurons, but also astrocytes and NG2 cells. Further, to specifically target single Gsx-2 subpallial progenitors and their derived cell-progeny in the piriform cortex, we used the UbC-(Gsx-2-hyPB)-StarTrack to perform an accurate analysis of their clonal relationships. Our results quantitatively delineate the adult clonal cell pattern from single subpallial E12-progenitors, focusing on glial cells. In summary, there is a temporal pattern in the assembly of the glial cell diversity in the piriform cortex, which also reveals spatio-temporal progenitor heterogeneity.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/278450
url http://hdl.handle.net/10261/278450
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105218RB-I00
http://dx.doi.org/10.3389/fnins.2022.825969
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers in Bioscience Publications
publisher.none.fl_str_mv Frontiers in Bioscience Publications
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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