Signatures of conformational stability and oxidation resistance in proteomes of pathogenic bacteria

Protein oxidation is known to compromise vital cellular functions. Therefore, invading pathogenic bacteria must resist damage inflicted by host defenses via reactive oxygen species. Using comparative genomics and experimental approaches, we provide multiple lines of evidence that proteins from patho...

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Detalles Bibliográficos
Autores: Vidovic, Anita, Supek, Fran, Nikolic, Andrea, Krisko, Anita
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Universitat Pompeu Fabra
Repositorio:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/69213
Acceso en línea:http://hdl.handle.net/10230/69213
Access Level:acceso abierto
Palabra clave:Proteòmica
Bacteris patògens
Oxidació
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spelling Signatures of conformational stability and oxidation resistance in proteomes of pathogenic bacteriaVidovic, Anita Supek, FranNikolic, AndreaKrisko, AnitaProteòmicaBacteris patògensOxidacióProtein oxidation is known to compromise vital cellular functions. Therefore, invading pathogenic bacteria must resist damage inflicted by host defenses via reactive oxygen species. Using comparative genomics and experimental approaches, we provide multiple lines of evidence that proteins from pathogenic bacteria have acquired resistance to oxidative stress by an increased conformational stability. Representative pathogens exhibited higher survival upon HSP90 inhibition and a less-oxidation-prone proteome. A proteome signature of the 46 pathogenic bacteria encompasses 14 physicochemical features related to increasing protein conformational stability. By purifying ten representative proteins, we demonstrate in vitro that proteins with a pathogen-like signature are more resistant to oxidative stress as a consequence of their increased conformational stability. A compositional signature of the pathogens’ proteomes allowed the design of protein fragments more resilient to both unfolding and carbonylation, validating the relationship between conformational stability and oxidability with implications for synthetic biology and antimicrobial strategies.The authors thank Miroslav Radman for stimulating discussions and Tea Copic for technical support. This work was supported by the Mediterranean Institute for Life Sciences. A.V. and A.N. are supported by the City of Split Fellowship for Excellence. The work of F.S. was supported in part by Marie Curie Actions; by grants 00980501 and 098-0000000-3168 of the Ministry of Science, Education, and Sport of Croatia; and by EU grant ICT-2013-612944 (MAESTRA). A.K. would like to thank Maria Marta Radman for her help with the graphics.Elsevier202520252014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/69213reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésCell Reports. 2014 Jun;7(5):1393-400info:eu-repo/grantAgreement/EC/FP7/612944© 2014 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).http://creativecommons.org/licenses/by-nc-nd/3.0/info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/692132026-06-12T07:21:37Z
dc.title.none.fl_str_mv Signatures of conformational stability and oxidation resistance in proteomes of pathogenic bacteria
title Signatures of conformational stability and oxidation resistance in proteomes of pathogenic bacteria
spellingShingle Signatures of conformational stability and oxidation resistance in proteomes of pathogenic bacteria
Vidovic, Anita
Proteòmica
Bacteris patògens
Oxidació
title_short Signatures of conformational stability and oxidation resistance in proteomes of pathogenic bacteria
title_full Signatures of conformational stability and oxidation resistance in proteomes of pathogenic bacteria
title_fullStr Signatures of conformational stability and oxidation resistance in proteomes of pathogenic bacteria
title_full_unstemmed Signatures of conformational stability and oxidation resistance in proteomes of pathogenic bacteria
title_sort Signatures of conformational stability and oxidation resistance in proteomes of pathogenic bacteria
dc.creator.none.fl_str_mv Vidovic, Anita
Supek, Fran
Nikolic, Andrea
Krisko, Anita
author Vidovic, Anita
author_facet Vidovic, Anita
Supek, Fran
Nikolic, Andrea
Krisko, Anita
author_role author
author2 Supek, Fran
Nikolic, Andrea
Krisko, Anita
author2_role author
author
author
dc.subject.none.fl_str_mv Proteòmica
Bacteris patògens
Oxidació
topic Proteòmica
Bacteris patògens
Oxidació
description Protein oxidation is known to compromise vital cellular functions. Therefore, invading pathogenic bacteria must resist damage inflicted by host defenses via reactive oxygen species. Using comparative genomics and experimental approaches, we provide multiple lines of evidence that proteins from pathogenic bacteria have acquired resistance to oxidative stress by an increased conformational stability. Representative pathogens exhibited higher survival upon HSP90 inhibition and a less-oxidation-prone proteome. A proteome signature of the 46 pathogenic bacteria encompasses 14 physicochemical features related to increasing protein conformational stability. By purifying ten representative proteins, we demonstrate in vitro that proteins with a pathogen-like signature are more resistant to oxidative stress as a consequence of their increased conformational stability. A compositional signature of the pathogens’ proteomes allowed the design of protein fragments more resilient to both unfolding and carbonylation, validating the relationship between conformational stability and oxidability with implications for synthetic biology and antimicrobial strategies.
publishDate 2014
dc.date.none.fl_str_mv 2014
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/69213
url http://hdl.handle.net/10230/69213
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Cell Reports. 2014 Jun;7(5):1393-400
info:eu-repo/grantAgreement/EC/FP7/612944
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/3.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/3.0/
eu_rights_str_mv openAccess
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application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositorio Digital de la UPF
instname:Universitat Pompeu Fabra
instname_str Universitat Pompeu Fabra
reponame_str Repositorio Digital de la UPF
collection Repositorio Digital de la UPF
repository.name.fl_str_mv
repository.mail.fl_str_mv
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