Overexpression of Ginkbilobin-2 homologous domain gene improves tolerance to Phytophthora cinnamomi in somatic embryos of Quercus suber

17 páginas, 5 figuras, 3 tablas Supplementary Information Te online version contains supplementary material available at https://doi.org/10.1038/s41598-024-70272-2.

Detalles Bibliográficos
Autores: Serrazina, Susana, Martínez-Santiago, María Teresa, Soudani, Serine, Candeias, Gonçalo, Berrocal-Lobo, Marta, Piñeiro, Pablo, Malhó, Rui, Lourenço Costa, Rita, Corredoira, Elena
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/373960
Acceso en línea:http://hdl.handle.net/10261/373960
Access Level:acceso abierto
Palabra clave:Agrobacterium tumefaciens
Cork oak
Cysteine-rich repeat secretory protein
Genetic transformation
In vitro tolerance assays
Oak decline disease
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spelling Overexpression of Ginkbilobin-2 homologous domain gene improves tolerance to Phytophthora cinnamomi in somatic embryos of Quercus suberSerrazina, SusanaMartínez-Santiago, María TeresaSoudani, SerineCandeias, GonçaloBerrocal-Lobo, MartaPiñeiro, PabloMalhó, RuiLourenço Costa, RitaCorredoira, ElenaAgrobacterium tumefaciensCork oakCysteine-rich repeat secretory proteinGenetic transformationIn vitro tolerance assaysOak decline disease17 páginas, 5 figuras, 3 tablas Supplementary Information Te online version contains supplementary material available at https://doi.org/10.1038/s41598-024-70272-2.In recent decades an extensive mortality and decline of Quercus suber populations mainly caused by Phytophthora cinnamomi has been observed. In the current study, a chestnut gene homologous to ginkbilobin-2 (Cast_Gnk2-like), which in Ginkgo biloba codifies an antifungal protein, was transferred into cork oak somatic embryos of three different embryogenic lines by Agrobacterium mediated transformation. The transformation efficiency varied on the genotype from 2.5 to 9.2%, and a total of 22 independent transformed lines were obtained. The presence of Cast_Gnk2-like gene in transgenic embryos was verified in all lines by PCR. The number of transgene copies was estimated by qPCR in embryogenic lines with high proliferation ability and it varied between 1 and 5. In addition, the expression levels of Cast_Gnk2-like gene were determined in the embryogenic lines, with higher levels in lines derived from the genotype ALM6-WT. Transgenic plants were obtained from all transgenic lines and evaluated after cold storage of the somatic embryos for 2 months and subsequent transfer to germination medium. In vitro tolerance tests made under controlled conditions and following zoospore treatment showed that plants overexpressing Cast_Gnk2-like gene improved tolerance against Pc when compared to wild type ones.Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. The research described in the present paper was partly supported by Ministerio de Ciencia, Innovación y Universidades (MICIN, Spain) through the project PID2020-112627RB-C33 (AEI/https://doi.org/10.13039/501100011033). SeS was funded by an international fellow from the UPM-Algerian Government from MHESR, Decree No. 14–196. SuS is being funded by an FCT contract in the project 2022.06990.PTDC (DOI: https://doi.org/10.54499/2022.06990.PTDC). At BioISI, work was supported by UIDB/04046/2020 (DOI: https://doi.org/10.54499/UIDB/04046/2020) and UIDP/04046/2020 (DOI: https://doi.org/10.54499/UIDP/04046/2020) Centre grants from FCT, Portugal.Peer reviewedNature Publishing GroupConsejo Superior de Investigaciones Científicas (España)Ministerio de Ciencia, Innovación y Universidades (España)Ministère de l'Enseignement Supérieur et de la Recherche Scientifique (Algerie)Fundação para a Ciência e a Tecnologia (Portugal)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2024202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/373960reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-112627RB-C33http://dx.doi.org/10.1038/s41598-024-70272-2Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3739602026-05-22T06:33:51Z
dc.title.none.fl_str_mv Overexpression of Ginkbilobin-2 homologous domain gene improves tolerance to Phytophthora cinnamomi in somatic embryos of Quercus suber
title Overexpression of Ginkbilobin-2 homologous domain gene improves tolerance to Phytophthora cinnamomi in somatic embryos of Quercus suber
spellingShingle Overexpression of Ginkbilobin-2 homologous domain gene improves tolerance to Phytophthora cinnamomi in somatic embryos of Quercus suber
Serrazina, Susana
Agrobacterium tumefaciens
Cork oak
Cysteine-rich repeat secretory protein
Genetic transformation
In vitro tolerance assays
Oak decline disease
title_short Overexpression of Ginkbilobin-2 homologous domain gene improves tolerance to Phytophthora cinnamomi in somatic embryos of Quercus suber
title_full Overexpression of Ginkbilobin-2 homologous domain gene improves tolerance to Phytophthora cinnamomi in somatic embryos of Quercus suber
title_fullStr Overexpression of Ginkbilobin-2 homologous domain gene improves tolerance to Phytophthora cinnamomi in somatic embryos of Quercus suber
title_full_unstemmed Overexpression of Ginkbilobin-2 homologous domain gene improves tolerance to Phytophthora cinnamomi in somatic embryos of Quercus suber
title_sort Overexpression of Ginkbilobin-2 homologous domain gene improves tolerance to Phytophthora cinnamomi in somatic embryos of Quercus suber
dc.creator.none.fl_str_mv Serrazina, Susana
Martínez-Santiago, María Teresa
Soudani, Serine
Candeias, Gonçalo
Berrocal-Lobo, Marta
Piñeiro, Pablo
Malhó, Rui
Lourenço Costa, Rita
Corredoira, Elena
author Serrazina, Susana
author_facet Serrazina, Susana
Martínez-Santiago, María Teresa
Soudani, Serine
Candeias, Gonçalo
Berrocal-Lobo, Marta
Piñeiro, Pablo
Malhó, Rui
Lourenço Costa, Rita
Corredoira, Elena
author_role author
author2 Martínez-Santiago, María Teresa
Soudani, Serine
Candeias, Gonçalo
Berrocal-Lobo, Marta
Piñeiro, Pablo
Malhó, Rui
Lourenço Costa, Rita
Corredoira, Elena
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Ministère de l'Enseignement Supérieur et de la Recherche Scientifique (Algerie)
Fundação para a Ciência e a Tecnologia (Portugal)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Agrobacterium tumefaciens
Cork oak
Cysteine-rich repeat secretory protein
Genetic transformation
In vitro tolerance assays
Oak decline disease
topic Agrobacterium tumefaciens
Cork oak
Cysteine-rich repeat secretory protein
Genetic transformation
In vitro tolerance assays
Oak decline disease
description 17 páginas, 5 figuras, 3 tablas Supplementary Information Te online version contains supplementary material available at https://doi.org/10.1038/s41598-024-70272-2.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/373960
url http://hdl.handle.net/10261/373960
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-112627RB-C33
http://dx.doi.org/10.1038/s41598-024-70272-2
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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