The effects of different cryoprotectants and the temperature of addition on the survival of red deer epididymal spermatozoa
With the aim of finding an ideal cryoprotectant in a suitable concentration for red deer epididymal spermatozoa conservation, we evaluated the effects of four most commonly used cryoprotectants (CPAs), Glycerol (G), Ethylene glycol (EG), Propylene glycol (PG), and Dimethyl sulfoxide (DMSO), on the s...
| Autores: | , , , , |
|---|---|
| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2005 |
| País: | España |
| Recursos: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/284115 |
| Acesso em linha: | http://hdl.handle.net/10261/284115 |
| Access Level: | acceso abierto |
| Palavra-chave: | DMSO Ethylene glycol Glycerol Propylene glycol Red deer Sperm |
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The effects of different cryoprotectants and the temperature of addition on the survival of red deer epididymal spermatozoaFernández-Santos, M. RocíoEsteso, Milagros C.Soler, Ana J.Montoro, VidalGarde, José JuliánDMSOEthylene glycolGlycerolPropylene glycolRed deerSpermWith the aim of finding an ideal cryoprotectant in a suitable concentration for red deer epididymal spermatozoa conservation, we evaluated the effects of four most commonly used cryoprotectants (CPAs), Glycerol (G), Ethylene glycol (EG), Propylene glycol (PG), and Dimethyl sulfoxide (DMSO), on the sperm survival. Besides, the effects of two temperatures of CPA addition − 22ºC (ambient temperature) and 5ºC − on sperm quality were also tested. For each temperature tested, sperm samples were evaluated after 0, 15, 30 and 60 min of spermatozoa exposition to CPAs. Thus, sperm quality was in vitro judged by microscopic assessments of individual sperm motility (SMI), and of plasma membrane (Viability) and acrosome (NAR) integrities. Overall, DMSO showed the highest toxicity for red deer epididymal spermatozoa, and glycerol the lowest. Thus, at 60 min of incubation SMI results showed that the toxicity to red deer epididymal spermatozoa of the four CPAs are in the following sequence: G ~ EG ~ PG < DMSO (‘less than’ symbol means P < 0.05, and approximate symbol means P = 0.08). Furthermore, our results also showed a differential response of acrosome membrane to temperature of CPAs addition. Regardless of the CPA used, statistically significant variations (P < 0.05) were found between the two temperatures of addition of CPAs for acrosome integrity, the best being 22ºC (NAR = 83.8% vs. 69.8%). These data indicate that sperm quality of red deer epididymal spermatozoa, in addition to be affected by the cryoprotectant, can also be influenced by the temperature at which CPAs are added prior to freezing.This work was sponsored by Spanish Ministry of Science and Technology (AGL2000-0671).Peer reviewedRoyal Veterinary CollegeMinisterio de Ciencia y Tecnología (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222005info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/284115reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2841152026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
The effects of different cryoprotectants and the temperature of addition on the survival of red deer epididymal spermatozoa |
| title |
The effects of different cryoprotectants and the temperature of addition on the survival of red deer epididymal spermatozoa |
| spellingShingle |
The effects of different cryoprotectants and the temperature of addition on the survival of red deer epididymal spermatozoa Fernández-Santos, M. Rocío DMSO Ethylene glycol Glycerol Propylene glycol Red deer Sperm |
| title_short |
The effects of different cryoprotectants and the temperature of addition on the survival of red deer epididymal spermatozoa |
| title_full |
The effects of different cryoprotectants and the temperature of addition on the survival of red deer epididymal spermatozoa |
| title_fullStr |
The effects of different cryoprotectants and the temperature of addition on the survival of red deer epididymal spermatozoa |
| title_full_unstemmed |
The effects of different cryoprotectants and the temperature of addition on the survival of red deer epididymal spermatozoa |
| title_sort |
The effects of different cryoprotectants and the temperature of addition on the survival of red deer epididymal spermatozoa |
| dc.creator.none.fl_str_mv |
Fernández-Santos, M. Rocío Esteso, Milagros C. Soler, Ana J. Montoro, Vidal Garde, José Julián |
| author |
Fernández-Santos, M. Rocío |
| author_facet |
Fernández-Santos, M. Rocío Esteso, Milagros C. Soler, Ana J. Montoro, Vidal Garde, José Julián |
| author_role |
author |
| author2 |
Esteso, Milagros C. Soler, Ana J. Montoro, Vidal Garde, José Julián |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
Ministerio de Ciencia y Tecnología (España) Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
DMSO Ethylene glycol Glycerol Propylene glycol Red deer Sperm |
| topic |
DMSO Ethylene glycol Glycerol Propylene glycol Red deer Sperm |
| description |
With the aim of finding an ideal cryoprotectant in a suitable concentration for red deer epididymal spermatozoa conservation, we evaluated the effects of four most commonly used cryoprotectants (CPAs), Glycerol (G), Ethylene glycol (EG), Propylene glycol (PG), and Dimethyl sulfoxide (DMSO), on the sperm survival. Besides, the effects of two temperatures of CPA addition − 22ºC (ambient temperature) and 5ºC − on sperm quality were also tested. For each temperature tested, sperm samples were evaluated after 0, 15, 30 and 60 min of spermatozoa exposition to CPAs. Thus, sperm quality was in vitro judged by microscopic assessments of individual sperm motility (SMI), and of plasma membrane (Viability) and acrosome (NAR) integrities. Overall, DMSO showed the highest toxicity for red deer epididymal spermatozoa, and glycerol the lowest. Thus, at 60 min of incubation SMI results showed that the toxicity to red deer epididymal spermatozoa of the four CPAs are in the following sequence: G ~ EG ~ PG < DMSO (‘less than’ symbol means P < 0.05, and approximate symbol means P = 0.08). Furthermore, our results also showed a differential response of acrosome membrane to temperature of CPAs addition. Regardless of the CPA used, statistically significant variations (P < 0.05) were found between the two temperatures of addition of CPAs for acrosome integrity, the best being 22ºC (NAR = 83.8% vs. 69.8%). These data indicate that sperm quality of red deer epididymal spermatozoa, in addition to be affected by the cryoprotectant, can also be influenced by the temperature at which CPAs are added prior to freezing. |
| publishDate |
2005 |
| dc.date.none.fl_str_mv |
2005 2022 2022 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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http://hdl.handle.net/10261/284115 |
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http://hdl.handle.net/10261/284115 |
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Inglés |
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Inglés |
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Sí |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Royal Veterinary College |
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Royal Veterinary College |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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