Nanoparticles incorporating pH-responsive surfactants as a viable approach to improve the intracellular drug delivery

The pH-responsive delivery systems have brought newadvances in the field of functional nanodevices and might allow more accurate and controllable delivery of specific cargoes, which is expected to result in promising applications in different clinical therapies. Here we describe a family of chitosan...

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Autores: Nogueira, Daniele R., Scheeren, Laís E., Vinardell Martínez-Hidalgo, Ma. Pilar, Mitjans Arnal, Montserrat, Infante Martínez-Pardo, Ma. Rosa, Rolim, Clarice M. B.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/97207
Acceso en línea:https://hdl.handle.net/2445/97207
Access Level:acceso abierto
Palabra clave:Quitosan
Nanopartícules
Agents tensioactius
Sistemes d'alliberament de medicaments
Hemòlisi
Toxicologia
Chitosan
Nanoparticles
Surface active agents
Drug delivery systems
Hemolysis
Toxicology
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oai_identifier_str oai:recercat.cat:2445/97207
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network_name_str España
repository_id_str
spelling Nanoparticles incorporating pH-responsive surfactants as a viable approach to improve the intracellular drug deliveryNogueira, Daniele R.Scheeren, Laís E.Vinardell Martínez-Hidalgo, Ma. PilarMitjans Arnal, MontserratInfante Martínez-Pardo, Ma. RosaRolim, Clarice M. B.QuitosanNanopartículesAgents tensioactiusSistemes d'alliberament de medicamentsHemòlisiToxicologiaChitosanNanoparticlesSurface active agentsDrug delivery systemsHemolysisToxicologyThe pH-responsive delivery systems have brought newadvances in the field of functional nanodevices and might allow more accurate and controllable delivery of specific cargoes, which is expected to result in promising applications in different clinical therapies. Here we describe a family of chitosan TPP (tripolyphosphate) nanoparticles (NPs) for intracellular drug delivery, which were designed using two pH-sensitive amino acid-based surfactants fromthe family Nα,Nε-dioctanoyl lysine as bioactive compounds. Lowand mediummolecularweight chitosan (LMW-CS and MMW-CS, respectively) were used for NP preparation, and it was observed that the size distribution for NPs with LMW-CS were smaller (~168 nm) than that for NPs prepared with MMW-CS (~310 nm). Hemolysis assay demonstrated the pH-dependent biomembrane disruptional capability of the constructed NPs. The nanostructures incorporating the surfactants cause negligible membrane permeabilization at pH 7.4. However, at acidic pH, prevailing in endosomes, membrane-destabilizing activity in an erythrocyte lysis assay became evident. When pH decreased to 6.6 and 5.4, hemolytic capability of chitosan NPs increased along with the raise of concentration. Furthermore, studies with cell culture showed that these pH-responsive NPs displayed low cytotoxic effects against 3T3 fibroblasts. The influence of chitosan molecular weight, chitosan to TPP weight ratio, nanoparticle size and nature of the surfactant counterion on the membrane-disruptive properties of nanoparticleswas discussed in detail. Altogether, the results achieved here showed that by inserting the lysine-based amphiphiles into chitosan NPs, pH-sensitive membranolytic and potentially endosomolytic nanocarriers were developed, which, therefore, demonstrated ideal feasibility for intracellular drug delivery.Elsevier B.V.2016201720152016info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion7 p.application/pdfhttps://hdl.handle.net/2445/97207Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: http://dx.doi.org/10.1016/j.msec.2015.07.036Materials Science & Engineering C-Materials For Biological Applications, 2015, vol. 57, p. 100-106http://dx.doi.org/10.1016/j.msec.2015.07.036cc-by-nc-nd (c) Elsevier B.V., 2015http://creativecommons.org/licenses/by-nc-nd/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/972072026-05-29T05:05:01Z
dc.title.none.fl_str_mv Nanoparticles incorporating pH-responsive surfactants as a viable approach to improve the intracellular drug delivery
title Nanoparticles incorporating pH-responsive surfactants as a viable approach to improve the intracellular drug delivery
spellingShingle Nanoparticles incorporating pH-responsive surfactants as a viable approach to improve the intracellular drug delivery
Nogueira, Daniele R.
Quitosan
Nanopartícules
Agents tensioactius
Sistemes d'alliberament de medicaments
Hemòlisi
Toxicologia
Chitosan
Nanoparticles
Surface active agents
Drug delivery systems
Hemolysis
Toxicology
title_short Nanoparticles incorporating pH-responsive surfactants as a viable approach to improve the intracellular drug delivery
title_full Nanoparticles incorporating pH-responsive surfactants as a viable approach to improve the intracellular drug delivery
title_fullStr Nanoparticles incorporating pH-responsive surfactants as a viable approach to improve the intracellular drug delivery
title_full_unstemmed Nanoparticles incorporating pH-responsive surfactants as a viable approach to improve the intracellular drug delivery
title_sort Nanoparticles incorporating pH-responsive surfactants as a viable approach to improve the intracellular drug delivery
dc.creator.none.fl_str_mv Nogueira, Daniele R.
Scheeren, Laís E.
Vinardell Martínez-Hidalgo, Ma. Pilar
Mitjans Arnal, Montserrat
Infante Martínez-Pardo, Ma. Rosa
Rolim, Clarice M. B.
author Nogueira, Daniele R.
author_facet Nogueira, Daniele R.
Scheeren, Laís E.
Vinardell Martínez-Hidalgo, Ma. Pilar
Mitjans Arnal, Montserrat
Infante Martínez-Pardo, Ma. Rosa
Rolim, Clarice M. B.
author_role author
author2 Scheeren, Laís E.
Vinardell Martínez-Hidalgo, Ma. Pilar
Mitjans Arnal, Montserrat
Infante Martínez-Pardo, Ma. Rosa
Rolim, Clarice M. B.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Quitosan
Nanopartícules
Agents tensioactius
Sistemes d'alliberament de medicaments
Hemòlisi
Toxicologia
Chitosan
Nanoparticles
Surface active agents
Drug delivery systems
Hemolysis
Toxicology
topic Quitosan
Nanopartícules
Agents tensioactius
Sistemes d'alliberament de medicaments
Hemòlisi
Toxicologia
Chitosan
Nanoparticles
Surface active agents
Drug delivery systems
Hemolysis
Toxicology
description The pH-responsive delivery systems have brought newadvances in the field of functional nanodevices and might allow more accurate and controllable delivery of specific cargoes, which is expected to result in promising applications in different clinical therapies. Here we describe a family of chitosan TPP (tripolyphosphate) nanoparticles (NPs) for intracellular drug delivery, which were designed using two pH-sensitive amino acid-based surfactants fromthe family Nα,Nε-dioctanoyl lysine as bioactive compounds. Lowand mediummolecularweight chitosan (LMW-CS and MMW-CS, respectively) were used for NP preparation, and it was observed that the size distribution for NPs with LMW-CS were smaller (~168 nm) than that for NPs prepared with MMW-CS (~310 nm). Hemolysis assay demonstrated the pH-dependent biomembrane disruptional capability of the constructed NPs. The nanostructures incorporating the surfactants cause negligible membrane permeabilization at pH 7.4. However, at acidic pH, prevailing in endosomes, membrane-destabilizing activity in an erythrocyte lysis assay became evident. When pH decreased to 6.6 and 5.4, hemolytic capability of chitosan NPs increased along with the raise of concentration. Furthermore, studies with cell culture showed that these pH-responsive NPs displayed low cytotoxic effects against 3T3 fibroblasts. The influence of chitosan molecular weight, chitosan to TPP weight ratio, nanoparticle size and nature of the surfactant counterion on the membrane-disruptive properties of nanoparticleswas discussed in detail. Altogether, the results achieved here showed that by inserting the lysine-based amphiphiles into chitosan NPs, pH-sensitive membranolytic and potentially endosomolytic nanocarriers were developed, which, therefore, demonstrated ideal feasibility for intracellular drug delivery.
publishDate 2015
dc.date.none.fl_str_mv 2015
2016
2016
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/97207
url https://hdl.handle.net/2445/97207
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: http://dx.doi.org/10.1016/j.msec.2015.07.036
Materials Science & Engineering C-Materials For Biological Applications, 2015, vol. 57, p. 100-106
http://dx.doi.org/10.1016/j.msec.2015.07.036
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Elsevier B.V., 2015
http://creativecommons.org/licenses/by-nc-nd/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Elsevier B.V., 2015
http://creativecommons.org/licenses/by-nc-nd/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 7 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Fisiologia)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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