Next-Generation Sequencing For Microbial Characterization Of Biovermiculations From A Sulfuric Acid Cave In Apulia (Italy)

4 páginas.-- 4 figuras.-- 18 referencias.-- Comunicación presentada en el 17th International Congress of Speleology, July 22–28, Sydney, NSW Australia

Detalles Bibliográficos
Autores: D’Angeli, Ilenia M., Waele, J. de, Ieva, Maria Grazia, Leuko, Stefan, Cappelletti, Martina, Parise, Mario, Jurado, Valme, Miller, A. Z., Sáiz-Jiménez, Cesáreo
Tipo de recurso: otro
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/162658
Acceso en línea:http://hdl.handle.net/10261/162658
Access Level:acceso abierto
Palabra clave:Geomicrobiology
Santa Cesarea Terme
Hypogenic cave
FESEM
16S rDNA
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spelling Next-Generation Sequencing For Microbial Characterization Of Biovermiculations From A Sulfuric Acid Cave In Apulia (Italy)D’Angeli, Ilenia M.Waele, J. deIeva, Maria GraziaLeuko, StefanCappelletti, MartinaParise, MarioJurado, ValmeMiller, A. Z.Sáiz-Jiménez, CesáreoGeomicrobiologySanta Cesarea TermeHypogenic caveFESEM16S rDNA4 páginas.-- 4 figuras.-- 18 referencias.-- Comunicación presentada en el 17th International Congress of Speleology, July 22–28, Sydney, NSW AustraliaSulfuric acid cave systems host abundant microbial communities that can colonize several environments displaying a variety of morphologies, i.e. white flamentous mats foating on the water surface, white creamy moonmilk deposits on the walls, and biovermiculations. Up to date, only few reports have described the microbiological aspects behind biovermiculation geomicrobiology of Italian sulfuric acid caves despite their overall abundance. Here, we present the frst characterization of biovermiculation microbial populations from the Santa Cesarea Terme (Apulia, Italy) using next-generation sequencing and feld emission scanning electron microscopy (FESEM) approaches. We focused our analysis on biovermiculations from Fetida Cave located along the Adriatic Sea coastline. Tis cave is at sea level, and moving from the entrance to its inner part, it is possible to observe a decrease of marine infuence accompanied by a corresponding increase in the acidic efect of the upwelling waters. Biovermiculations are copiously observed covering the ceiling and walls mainly in the inner and middle environments of the cave, while they are absent near the entrance. Biovermiculations have a widespread range of colors going from grey to dark brown with an overall slimy appearance; dendritic morphologies alternate to very dense wall-covering sheets. Total DNA was extracted from each sample and 16S rDNA sequences were analyzed through Ilumina MiSeq platform. Te main lineages found in all the samples included Gammaproteobacteria, Betaproteobacteria, Alphaproteobacteria, Bacteroidetes, Planctomycetes, Actinobacteria and Acidobacteria. In particular, the samples from the inner part of the cave had the highest abundance of Acidobacteria and showed the presence of Epsilonproteobacteria that can be related to bacterial sulfuroxidizing activity. FESEM images revealed microbial mats composed of flamentous cells including Actinobacteria and reticulated flaments of unknown origin, as well as prosthecate bacteria. Tis study shows that the biovermiculation microbial communities from Fetida cave is in accordance with previous data reported n acidic caves from Italy and support the role of Epsilonproteobacteria typically occurring in sulfdic environments.Many thanks Santa Cesarea Terme spa, for the availability to visit caves and sample geochemical and microbiological materials. Tanks to Lee Florea that helped us in monitoring geochemical parameters and in samples collection. Financial support from the Spanish project CGL2016-75590-P is acknowledged.Peer reviewedAustralian Speleological FederationMinisterio de Economía y Competitividad (España)201820182017info:eu-repo/semantics/otherhttp://purl.org/coar/resource_type/c_3248Publisher's versioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bookParthttp://hdl.handle.net/10261/162658reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CGL2016-75590-PNoinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1626582026-05-22T06:33:51Z
dc.title.none.fl_str_mv Next-Generation Sequencing For Microbial Characterization Of Biovermiculations From A Sulfuric Acid Cave In Apulia (Italy)
title Next-Generation Sequencing For Microbial Characterization Of Biovermiculations From A Sulfuric Acid Cave In Apulia (Italy)
spellingShingle Next-Generation Sequencing For Microbial Characterization Of Biovermiculations From A Sulfuric Acid Cave In Apulia (Italy)
D’Angeli, Ilenia M.
Geomicrobiology
Santa Cesarea Terme
Hypogenic cave
FESEM
16S rDNA
title_short Next-Generation Sequencing For Microbial Characterization Of Biovermiculations From A Sulfuric Acid Cave In Apulia (Italy)
title_full Next-Generation Sequencing For Microbial Characterization Of Biovermiculations From A Sulfuric Acid Cave In Apulia (Italy)
title_fullStr Next-Generation Sequencing For Microbial Characterization Of Biovermiculations From A Sulfuric Acid Cave In Apulia (Italy)
title_full_unstemmed Next-Generation Sequencing For Microbial Characterization Of Biovermiculations From A Sulfuric Acid Cave In Apulia (Italy)
title_sort Next-Generation Sequencing For Microbial Characterization Of Biovermiculations From A Sulfuric Acid Cave In Apulia (Italy)
dc.creator.none.fl_str_mv D’Angeli, Ilenia M.
Waele, J. de
Ieva, Maria Grazia
Leuko, Stefan
Cappelletti, Martina
Parise, Mario
Jurado, Valme
Miller, A. Z.
Sáiz-Jiménez, Cesáreo
author D’Angeli, Ilenia M.
author_facet D’Angeli, Ilenia M.
Waele, J. de
Ieva, Maria Grazia
Leuko, Stefan
Cappelletti, Martina
Parise, Mario
Jurado, Valme
Miller, A. Z.
Sáiz-Jiménez, Cesáreo
author_role author
author2 Waele, J. de
Ieva, Maria Grazia
Leuko, Stefan
Cappelletti, Martina
Parise, Mario
Jurado, Valme
Miller, A. Z.
Sáiz-Jiménez, Cesáreo
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
dc.subject.none.fl_str_mv Geomicrobiology
Santa Cesarea Terme
Hypogenic cave
FESEM
16S rDNA
topic Geomicrobiology
Santa Cesarea Terme
Hypogenic cave
FESEM
16S rDNA
description 4 páginas.-- 4 figuras.-- 18 referencias.-- Comunicación presentada en el 17th International Congress of Speleology, July 22–28, Sydney, NSW Australia
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/other
http://purl.org/coar/resource_type/c_3248
Publisher's version
info:eu-repo/semantics/publishedVersion
dc.type.openaire.fl_str_mv info:eu-repo/semantics/bookPart
format other
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/162658
url http://hdl.handle.net/10261/162658
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CGL2016-75590-P
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Australian Speleological Federation
publisher.none.fl_str_mv Australian Speleological Federation
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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