Participation of hepcidins in the inflammatory response triggers by λ-carrageenin in gilthead seabream (Sparus aurata)

The role of hepcidins, antimicrobial peptides involved in iron metabolism, immunity, and inflammation is studied. First, gilthead seabream (Sparus aurata L.) head-kidney leucocytes (HKLs) were incubated with λ-carrageenin to study the expression of hepcidin and iron metabolism-related genes. The exp...

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Detalles Bibliográficos
Autores: Campos-Sánchez, José Carlos, Serna-Duque, Jhon A., Alburquerque, Carmen, Esteban Abad, María de los Ángeles, Guardiola Abellán, Francisco Antonio
Tipo de recurso: conjunto de datos
Fecha de publicación:2023
País:España
Institución:Universidad de Murcia
Repositorio:DIGITUM. Depósito Digital Institucional de la Universidad de Murcia
OAI Identifier:oai:digitum.um.es:10201/130524
Acceso en línea:http://hdl.handle.net/10201/130524
Access Level:acceso abierto
Palabra clave:Hepcidins
Host defense peptides
Skin inflammation
Iron metabolism
Carrageenin
Gilthead seabream (Sparus aurata).
CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología
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repository_id_str
spelling Participation of hepcidins in the inflammatory response triggers by λ-carrageenin in gilthead seabream (Sparus aurata)Campos-Sánchez, José CarlosSerna-Duque, Jhon A.Alburquerque, CarmenEsteban Abad, María de los ÁngelesGuardiola Abellán, Francisco AntonioHepcidinsHost defense peptidesSkin inflammationIron metabolismCarrageeninGilthead seabream (Sparus aurata).CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. CitologíaThe role of hepcidins, antimicrobial peptides involved in iron metabolism, immunity, and inflammation is studied. First, gilthead seabream (Sparus aurata L.) head-kidney leucocytes (HKLs) were incubated with λ-carrageenin to study the expression of hepcidin and iron metabolism-related genes. The expression of most of the genes studied was up-regulated, whereas the ferroportin gene (slc40a) was down-regulated in HKLs incubated with λ-carrageenin. In the second part of the study, seabream specimens were injected with λ-carrageenin or buffer (control). The expression of the same genes was evaluated in the head kidney, liver, and skin at different time points after injection. The expression of Hamp1m, ferritin b and ferroportin genes (hamp1, fthb, and slc40a) was up-regulated in the head kidney of fish from the λ-carrageenin group, while the expression of Hamp2C and Hamp2E genes (hamp2.3 and hamp2.7) was down-regulated. In the liver, the expression of hamp1, ferritin a (ftha), slc40a, Hamp2J and Hamp2D (hamp2.5/6) genes was down-regulated in the λ-carrageenin group. In the skin, the expression of hamp1 and (Hamp2A Hamp2C) hamp2.1/3/4 genes was up-regulated in the λ-carrageenin group. A bioinformatic analysis was performed to predict the presence of transcription factor binding sites in the promoter region of hepcidins. Structural and physicochemical variations were found in the different mature hepcidin peptides. This study sheds light on the poorly understood roles of hepcidins in fish. The results provide insight into the regulatory mechanisms of inflammation in fish and could contribute to the development of new strategies for treat inflammation in farm animals.Biología Celular e Histología202320232023info:eu-repo/semantics/datasetapplication/vnd.ms-excel4application/vnd.ms-excelhttp://hdl.handle.net/10201/130524reponame:DIGITUM. Depósito Digital Institucional de la Universidad de Murciainstname:Universidad de MurciaInglésJCCS has a postdoctoral contract within the “Program for the retraining of the Spanish university system during the three-year period 2021-2023 – Margarita Salas (R-1593/2022)”. JASD has a PhD grant (FPU19/02192). This work was supported by the Spanish project PID2020-113637RB-C21 funded by MCIN/AEI, by the Comunidad Autónoma Región de Murcia-Fundación Séneca (ThinkInAzul). España. Unión europea”. This research forms part of the ThinkInAzul programme supported by MCIN with funding from European Union Next Generation EU (PRTR-C17. I1) and by the Comunidad Autónoma de la Región de Murcia-Fundación Séneca (Spain).info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:digitum.um.es:10201/1305242026-05-27T12:40:41Z
dc.title.none.fl_str_mv Participation of hepcidins in the inflammatory response triggers by λ-carrageenin in gilthead seabream (Sparus aurata)
title Participation of hepcidins in the inflammatory response triggers by λ-carrageenin in gilthead seabream (Sparus aurata)
spellingShingle Participation of hepcidins in the inflammatory response triggers by λ-carrageenin in gilthead seabream (Sparus aurata)
Campos-Sánchez, José Carlos
Hepcidins
Host defense peptides
Skin inflammation
Iron metabolism
Carrageenin
Gilthead seabream (Sparus aurata).
CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología
title_short Participation of hepcidins in the inflammatory response triggers by λ-carrageenin in gilthead seabream (Sparus aurata)
title_full Participation of hepcidins in the inflammatory response triggers by λ-carrageenin in gilthead seabream (Sparus aurata)
title_fullStr Participation of hepcidins in the inflammatory response triggers by λ-carrageenin in gilthead seabream (Sparus aurata)
title_full_unstemmed Participation of hepcidins in the inflammatory response triggers by λ-carrageenin in gilthead seabream (Sparus aurata)
title_sort Participation of hepcidins in the inflammatory response triggers by λ-carrageenin in gilthead seabream (Sparus aurata)
dc.creator.none.fl_str_mv Campos-Sánchez, José Carlos
Serna-Duque, Jhon A.
Alburquerque, Carmen
Esteban Abad, María de los Ángeles
Guardiola Abellán, Francisco Antonio
author Campos-Sánchez, José Carlos
author_facet Campos-Sánchez, José Carlos
Serna-Duque, Jhon A.
Alburquerque, Carmen
Esteban Abad, María de los Ángeles
Guardiola Abellán, Francisco Antonio
author_role author
author2 Serna-Duque, Jhon A.
Alburquerque, Carmen
Esteban Abad, María de los Ángeles
Guardiola Abellán, Francisco Antonio
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Biología Celular e Histología
dc.subject.none.fl_str_mv Hepcidins
Host defense peptides
Skin inflammation
Iron metabolism
Carrageenin
Gilthead seabream (Sparus aurata).
CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología
topic Hepcidins
Host defense peptides
Skin inflammation
Iron metabolism
Carrageenin
Gilthead seabream (Sparus aurata).
CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología
description The role of hepcidins, antimicrobial peptides involved in iron metabolism, immunity, and inflammation is studied. First, gilthead seabream (Sparus aurata L.) head-kidney leucocytes (HKLs) were incubated with λ-carrageenin to study the expression of hepcidin and iron metabolism-related genes. The expression of most of the genes studied was up-regulated, whereas the ferroportin gene (slc40a) was down-regulated in HKLs incubated with λ-carrageenin. In the second part of the study, seabream specimens were injected with λ-carrageenin or buffer (control). The expression of the same genes was evaluated in the head kidney, liver, and skin at different time points after injection. The expression of Hamp1m, ferritin b and ferroportin genes (hamp1, fthb, and slc40a) was up-regulated in the head kidney of fish from the λ-carrageenin group, while the expression of Hamp2C and Hamp2E genes (hamp2.3 and hamp2.7) was down-regulated. In the liver, the expression of hamp1, ferritin a (ftha), slc40a, Hamp2J and Hamp2D (hamp2.5/6) genes was down-regulated in the λ-carrageenin group. In the skin, the expression of hamp1 and (Hamp2A Hamp2C) hamp2.1/3/4 genes was up-regulated in the λ-carrageenin group. A bioinformatic analysis was performed to predict the presence of transcription factor binding sites in the promoter region of hepcidins. Structural and physicochemical variations were found in the different mature hepcidin peptides. This study sheds light on the poorly understood roles of hepcidins in fish. The results provide insight into the regulatory mechanisms of inflammation in fish and could contribute to the development of new strategies for treat inflammation in farm animals.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
format dataset
dc.identifier.none.fl_str_mv http://hdl.handle.net/10201/130524
url http://hdl.handle.net/10201/130524
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv JCCS has a postdoctoral contract within the “Program for the retraining of the Spanish university system during the three-year period 2021-2023 – Margarita Salas (R-1593/2022)”. JASD has a PhD grant (FPU19/02192). This work was supported by the Spanish project PID2020-113637RB-C21 funded by MCIN/AEI, by the Comunidad Autónoma Región de Murcia-Fundación Séneca (ThinkInAzul). España. Unión europea”. This research forms part of the ThinkInAzul programme supported by MCIN with funding from European Union Next Generation EU (PRTR-C17. I1) and by the Comunidad Autónoma de la Región de Murcia-Fundación Séneca (Spain).
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/vnd.ms-excel
4
application/vnd.ms-excel
dc.source.none.fl_str_mv reponame:DIGITUM. Depósito Digital Institucional de la Universidad de Murcia
instname:Universidad de Murcia
instname_str Universidad de Murcia
reponame_str DIGITUM. Depósito Digital Institucional de la Universidad de Murcia
collection DIGITUM. Depósito Digital Institucional de la Universidad de Murcia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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