LeuO is a global regulator of gene expression in Salmonella enterica serovar Typhimurium

We report the first investigation of the binding of the Salmonella enterica LeuO LysR-type transcription regulator to its genomic targets in vivo. Chromatin-immunoprecipitation-on-chip identified 178 LeuO binding sites on the chromosome of S.enterica serovar Typhimurium strain SL1344. These sites we...

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Autores: Dillon, Shane C., Espinosa Alfaro, Elena, Hokamp, Karsten, Ussery, David W., Casadesús Pursals, Josep, Dorman, Charles J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/133410
Acceso en línea:https://hdl.handle.net/11441/133410
https://doi.org/10.1111/j.1365-2958.2012.08162.x
Access Level:acceso abierto
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spelling LeuO is a global regulator of gene expression in Salmonella enterica serovar TyphimuriumDillon, Shane C.Espinosa Alfaro, ElenaHokamp, KarstenUssery, David W.Casadesús Pursals, JosepDorman, Charles J.We report the first investigation of the binding of the Salmonella enterica LeuO LysR-type transcription regulator to its genomic targets in vivo. Chromatin-immunoprecipitation-on-chip identified 178 LeuO binding sites on the chromosome of S.enterica serovar Typhimurium strain SL1344. These sites were distributed across both the core and the horizontally acquired genome, and included housekeeping genes and genes known to contribute to virulence. Sixty-eight LeuO targets were co-bound by the global repressor protein, H-NS. Thus, while LeuO may function as an H-NS antagonist, these functions are unlikely to involve displacement of H-NS. RNA polymerase bound 173 of the 178 LeuO targets, consistent with LeuO being a transcription regulator. Thus, LeuO targets two classes of genes, those that are bound by H-NS and those that are not bound by H-NS. LeuO binding site analysis revealed a logo conforming to the TN11A motif common to LysR-type transcription factors. It differed in some details from a motif that we composed for Escherichia coli LeuO binding sites; 1263 and 1094 LeuO binding site locations were predicted in the S.Typhimurium SL1344 and E.coli MG1655 genomes respectively. Despite differences in motif composition, many LeuO target genes were common to both species. Thus, LeuO is likely to be a more important global regulator than previously suspected.Science Foundation Ireland 07/IN.1/B918Ministerio de Ciencia e Innovación BIO2010-15023, CSD2008-00013Wiley-BlackwellGenéticaScience Foundation IrelandMinisterio de Ciencia e Innovación (MICIN). España2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/133410https://doi.org/10.1111/j.1365-2958.2012.08162.xreponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésMolecular Microbiology, 85 (6), 1072-1089.07/IN.1/B918BIO2010-15023CSD2008-00013https://doi.org/10.1111/j.1365-2958.2012.08162.xinfo:eu-repo/semantics/openAccessoai:idus.us.es:11441/1334102026-06-17T12:51:07Z
dc.title.none.fl_str_mv LeuO is a global regulator of gene expression in Salmonella enterica serovar Typhimurium
title LeuO is a global regulator of gene expression in Salmonella enterica serovar Typhimurium
spellingShingle LeuO is a global regulator of gene expression in Salmonella enterica serovar Typhimurium
Dillon, Shane C.
title_short LeuO is a global regulator of gene expression in Salmonella enterica serovar Typhimurium
title_full LeuO is a global regulator of gene expression in Salmonella enterica serovar Typhimurium
title_fullStr LeuO is a global regulator of gene expression in Salmonella enterica serovar Typhimurium
title_full_unstemmed LeuO is a global regulator of gene expression in Salmonella enterica serovar Typhimurium
title_sort LeuO is a global regulator of gene expression in Salmonella enterica serovar Typhimurium
dc.creator.none.fl_str_mv Dillon, Shane C.
Espinosa Alfaro, Elena
Hokamp, Karsten
Ussery, David W.
Casadesús Pursals, Josep
Dorman, Charles J.
author Dillon, Shane C.
author_facet Dillon, Shane C.
Espinosa Alfaro, Elena
Hokamp, Karsten
Ussery, David W.
Casadesús Pursals, Josep
Dorman, Charles J.
author_role author
author2 Espinosa Alfaro, Elena
Hokamp, Karsten
Ussery, David W.
Casadesús Pursals, Josep
Dorman, Charles J.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Genética
Science Foundation Ireland
Ministerio de Ciencia e Innovación (MICIN). España
description We report the first investigation of the binding of the Salmonella enterica LeuO LysR-type transcription regulator to its genomic targets in vivo. Chromatin-immunoprecipitation-on-chip identified 178 LeuO binding sites on the chromosome of S.enterica serovar Typhimurium strain SL1344. These sites were distributed across both the core and the horizontally acquired genome, and included housekeeping genes and genes known to contribute to virulence. Sixty-eight LeuO targets were co-bound by the global repressor protein, H-NS. Thus, while LeuO may function as an H-NS antagonist, these functions are unlikely to involve displacement of H-NS. RNA polymerase bound 173 of the 178 LeuO targets, consistent with LeuO being a transcription regulator. Thus, LeuO targets two classes of genes, those that are bound by H-NS and those that are not bound by H-NS. LeuO binding site analysis revealed a logo conforming to the TN11A motif common to LysR-type transcription factors. It differed in some details from a motif that we composed for Escherichia coli LeuO binding sites; 1263 and 1094 LeuO binding site locations were predicted in the S.Typhimurium SL1344 and E.coli MG1655 genomes respectively. Despite differences in motif composition, many LeuO target genes were common to both species. Thus, LeuO is likely to be a more important global regulator than previously suspected.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/133410
https://doi.org/10.1111/j.1365-2958.2012.08162.x
url https://hdl.handle.net/11441/133410
https://doi.org/10.1111/j.1365-2958.2012.08162.x
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Molecular Microbiology, 85 (6), 1072-1089.
07/IN.1/B918
BIO2010-15023
CSD2008-00013
https://doi.org/10.1111/j.1365-2958.2012.08162.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
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