Radiation-induced g-H2AX foci in different leukocyte subpopulations
To evaluate the γ-H2AX foci in peripheral mononucleated cells (PBMCs) from an exposed person is considered as an early assay for biological dosimetry. However, overdispersion in the γ-H2AX foci cell distribution is generally reported. This make it difficult to use this biomarker to assess non-homoge...
| Autores: | , , , , |
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| Tipo de recurso: | conjunto de datos |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:268847 |
| Acceso en línea: | https://ddd.uab.cat/record/268847 https://dx.doi.org/urn:doi:10.5565/ddd.uab.cat/268847 |
| Access Level: | acceso abierto |
| Palabra clave: | γ-H2AX foci distribution Peripheral mononuleated cells Ionizing radiation |
| Sumario: | To evaluate the γ-H2AX foci in peripheral mononucleated cells (PBMCs) from an exposed person is considered as an early assay for biological dosimetry. However, overdispersion in the γ-H2AX foci cell distribution is generally reported. This make it difficult to use this biomarker to assess non-homogenoeus exposures. The data of this research aims to evaluate: A) the possible differences in the radiosensitivity of the different cell subtypes present in the PBMCs, and B) to evaluate the distribution of γ-H2AX foci per cell in each cell subtype. Peripheral blood samples from three healthy donors were obtained to separate total PBMCs, and CD3+, C4+, CD8+, CD19+, and CD56+ cells. Cells were irradiated at 1 and 2 Gy and incubated at 37 °C for 1, 2, 4, and 24 h. Sham-irradiated cells were also analyzed. γ-H2AX foci were detected by microspcopy after immunofluorescence staining. For each condition, 250 nuclei were considered. |
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