mRNA expression analysis of the SUMO pathway genes in the adult mouse retina

Sumoylation is a reversible post-translational modification that regulates different cellular processes by conjugation/deconjugation of SUMO moieties to target proteins. Most work on the functional relevance of SUMO has focused on cell cycle, DNA repair and cancer in cultured cells, but data on the...

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Detalhes bibliográficos
Autores: Abad-Morales, Víctor, Domènech, Elena B., Garanto Iglesias, Alejandro, Marfany i Nadal, Gemma
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Recursos:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/108642
Acesso em linha:https://hdl.handle.net/2445/108642
Access Level:acceso abierto
Palavra-chave:Ubiqüitina
Retina
Expressió gènica
Ratolins (Animals de laboratori)
Ubiquitin
Gene expression
Mice (Laboratory animals)
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spelling mRNA expression analysis of the SUMO pathway genes in the adult mouse retinaAbad-Morales, VíctorDomènech, Elena B.Garanto Iglesias, AlejandroMarfany i Nadal, GemmaUbiqüitinaRetinaExpressió gènicaRatolins (Animals de laboratori)UbiquitinRetinaGene expressionMice (Laboratory animals)Sumoylation is a reversible post-translational modification that regulates different cellular processes by conjugation/deconjugation of SUMO moieties to target proteins. Most work on the functional relevance of SUMO has focused on cell cycle, DNA repair and cancer in cultured cells, but data on the inter-dependence of separate components of the SUMO pathway in highly specialized tissues, such as the retina, is still scanty. Nonetheless, several retinal transcription factors (TFs) relevant for cone and rod fate, as well as some circadian rhythm regulators, are regulated by sumoylation. Here we present a comprehensive survey of SUMO pathway gene expression in the murine retina by quantitative RT-PCR and in situ hybridization (ISH). The mRNA expression levels were quantified in retinas obtained under four different light/dark conditions, revealing distinct levels of gene expression. In addition, a SUMO pathway retinal gene atlas based on the mRNA expression pattern was drawn. Although most genes are ubiquitously expressed, some patterns could be defined in a first step to determine its biological significance and interdependence. The wide expression of the SUMO pathway genes, the transcriptional response under several light/dark conditions, and the diversity of expression patterns in different cell layers clearly support sumoylation as a relevant post-translational modification in the retina. This expression atlas intends to be a reference framework for retinal researchers and to depict a more comprehensive view of the SUMO-regulated processes in the retina.The Company of Biologists2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/108642Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1242/bio.201410645Biology Open, 2015, vol. 4, num. 2, p. 224-232https://doi.org/10.1242/bio.201410645cc-by (c) Abad-Morales, Víctor et al., 2015http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1086422026-05-27T06:46:51Z
dc.title.none.fl_str_mv mRNA expression analysis of the SUMO pathway genes in the adult mouse retina
title mRNA expression analysis of the SUMO pathway genes in the adult mouse retina
spellingShingle mRNA expression analysis of the SUMO pathway genes in the adult mouse retina
Abad-Morales, Víctor
Ubiqüitina
Retina
Expressió gènica
Ratolins (Animals de laboratori)
Ubiquitin
Retina
Gene expression
Mice (Laboratory animals)
title_short mRNA expression analysis of the SUMO pathway genes in the adult mouse retina
title_full mRNA expression analysis of the SUMO pathway genes in the adult mouse retina
title_fullStr mRNA expression analysis of the SUMO pathway genes in the adult mouse retina
title_full_unstemmed mRNA expression analysis of the SUMO pathway genes in the adult mouse retina
title_sort mRNA expression analysis of the SUMO pathway genes in the adult mouse retina
dc.creator.none.fl_str_mv Abad-Morales, Víctor
Domènech, Elena B.
Garanto Iglesias, Alejandro
Marfany i Nadal, Gemma
author Abad-Morales, Víctor
author_facet Abad-Morales, Víctor
Domènech, Elena B.
Garanto Iglesias, Alejandro
Marfany i Nadal, Gemma
author_role author
author2 Domènech, Elena B.
Garanto Iglesias, Alejandro
Marfany i Nadal, Gemma
author2_role author
author
author
dc.subject.none.fl_str_mv Ubiqüitina
Retina
Expressió gènica
Ratolins (Animals de laboratori)
Ubiquitin
Retina
Gene expression
Mice (Laboratory animals)
topic Ubiqüitina
Retina
Expressió gènica
Ratolins (Animals de laboratori)
Ubiquitin
Retina
Gene expression
Mice (Laboratory animals)
description Sumoylation is a reversible post-translational modification that regulates different cellular processes by conjugation/deconjugation of SUMO moieties to target proteins. Most work on the functional relevance of SUMO has focused on cell cycle, DNA repair and cancer in cultured cells, but data on the inter-dependence of separate components of the SUMO pathway in highly specialized tissues, such as the retina, is still scanty. Nonetheless, several retinal transcription factors (TFs) relevant for cone and rod fate, as well as some circadian rhythm regulators, are regulated by sumoylation. Here we present a comprehensive survey of SUMO pathway gene expression in the murine retina by quantitative RT-PCR and in situ hybridization (ISH). The mRNA expression levels were quantified in retinas obtained under four different light/dark conditions, revealing distinct levels of gene expression. In addition, a SUMO pathway retinal gene atlas based on the mRNA expression pattern was drawn. Although most genes are ubiquitously expressed, some patterns could be defined in a first step to determine its biological significance and interdependence. The wide expression of the SUMO pathway genes, the transcriptional response under several light/dark conditions, and the diversity of expression patterns in different cell layers clearly support sumoylation as a relevant post-translational modification in the retina. This expression atlas intends to be a reference framework for retinal researchers and to depict a more comprehensive view of the SUMO-regulated processes in the retina.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/108642
url https://hdl.handle.net/2445/108642
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1242/bio.201410645
Biology Open, 2015, vol. 4, num. 2, p. 224-232
https://doi.org/10.1242/bio.201410645
dc.rights.none.fl_str_mv cc-by (c) Abad-Morales, Víctor et al., 2015
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Abad-Morales, Víctor et al., 2015
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The Company of Biologists
publisher.none.fl_str_mv The Company of Biologists
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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