Experimental validation of seminal miR‐31‐5p as biomarker for azoospermia and evaluation of the effect of preanalytical variables

Background and objectives: Predicting the origin of azoospermia with non-invasive biomarkers is clinically relevant for determining the chance of successful sperm retrieval from the testes before attempting ART. Here, the semen small extracellular vesicle (sEV) microRNA (miRNA) miR-31-5p-based bioma...

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Detalhes bibliográficos
Autores: Plata Peña, Laura, López Rodrigo, Olga, Bassas, Lluís, Larriba, Sara
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2022
País:España
Recursos:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/188943
Acesso em linha:https://hdl.handle.net/2445/188943
Access Level:acceso abierto
Palavra-chave:Marcadors bioquímics
Andrologia
Vesícules seminals
Biochemical markers
Andrology
Seminal vesicles
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spelling Experimental validation of seminal miR‐31‐5p as biomarker for azoospermia and evaluation of the effect of preanalytical variablesPlata Peña, LauraLópez Rodrigo, OlgaBassas, LluísLarriba, SaraMarcadors bioquímicsAndrologiaVesícules seminalsBiochemical markersAndrologySeminal vesiclesBackground and objectives: Predicting the origin of azoospermia with non-invasive biomarkers is clinically relevant for determining the chance of successful sperm retrieval from the testes before attempting ART. Here, the semen small extracellular vesicle (sEV) microRNA (miRNA) miR-31-5p-based biomarker test to distinguish obstructive (OA) from secretory azoospermia (SA) (previously described by our group) is validated for clinical use and additionally sample source [seminal sEVs vs total seminal plasma (SP)] as a preanalytical variable is considered to optimize the procedure. Results and discussion: Our results provide evidence that altered miR-31-5p expression can be determined both from EVs and from the whole SP to discriminate OA from SA azoospermic samples. Not only have we validated this miRNA-based molecular model as a clinically useful test for predicting the origin of azoospermia in a sample from azoospermic individuals, but additionally, and more interestingly for the clinicians, we have evidenced its usefulness for predicting the presence of spermatogenic failure in azoospermic patients with FSH values <10 IU/L as a sensitive and specific biomarker (AUC>0.88; p-value<0.006). This article is protected by copyright. All rights reserved. Keywords: Seminal plasma; azoospermia; extracellular vesicles; miRNA; non-invasive biomarker.Wiley2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/188943Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésversió postprint del document publicat a: https://doi.org/10.1111/andr.13286Andrology, 2022https://doi.org/10.1111/andr.13286(c) American Society of Andrology and European Academy of Andrology, 2022info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1889432026-05-27T06:46:51Z
dc.title.none.fl_str_mv Experimental validation of seminal miR‐31‐5p as biomarker for azoospermia and evaluation of the effect of preanalytical variables
title Experimental validation of seminal miR‐31‐5p as biomarker for azoospermia and evaluation of the effect of preanalytical variables
spellingShingle Experimental validation of seminal miR‐31‐5p as biomarker for azoospermia and evaluation of the effect of preanalytical variables
Plata Peña, Laura
Marcadors bioquímics
Andrologia
Vesícules seminals
Biochemical markers
Andrology
Seminal vesicles
title_short Experimental validation of seminal miR‐31‐5p as biomarker for azoospermia and evaluation of the effect of preanalytical variables
title_full Experimental validation of seminal miR‐31‐5p as biomarker for azoospermia and evaluation of the effect of preanalytical variables
title_fullStr Experimental validation of seminal miR‐31‐5p as biomarker for azoospermia and evaluation of the effect of preanalytical variables
title_full_unstemmed Experimental validation of seminal miR‐31‐5p as biomarker for azoospermia and evaluation of the effect of preanalytical variables
title_sort Experimental validation of seminal miR‐31‐5p as biomarker for azoospermia and evaluation of the effect of preanalytical variables
dc.creator.none.fl_str_mv Plata Peña, Laura
López Rodrigo, Olga
Bassas, Lluís
Larriba, Sara
author Plata Peña, Laura
author_facet Plata Peña, Laura
López Rodrigo, Olga
Bassas, Lluís
Larriba, Sara
author_role author
author2 López Rodrigo, Olga
Bassas, Lluís
Larriba, Sara
author2_role author
author
author
dc.subject.none.fl_str_mv Marcadors bioquímics
Andrologia
Vesícules seminals
Biochemical markers
Andrology
Seminal vesicles
topic Marcadors bioquímics
Andrologia
Vesícules seminals
Biochemical markers
Andrology
Seminal vesicles
description Background and objectives: Predicting the origin of azoospermia with non-invasive biomarkers is clinically relevant for determining the chance of successful sperm retrieval from the testes before attempting ART. Here, the semen small extracellular vesicle (sEV) microRNA (miRNA) miR-31-5p-based biomarker test to distinguish obstructive (OA) from secretory azoospermia (SA) (previously described by our group) is validated for clinical use and additionally sample source [seminal sEVs vs total seminal plasma (SP)] as a preanalytical variable is considered to optimize the procedure. Results and discussion: Our results provide evidence that altered miR-31-5p expression can be determined both from EVs and from the whole SP to discriminate OA from SA azoospermic samples. Not only have we validated this miRNA-based molecular model as a clinically useful test for predicting the origin of azoospermia in a sample from azoospermic individuals, but additionally, and more interestingly for the clinicians, we have evidenced its usefulness for predicting the presence of spermatogenic failure in azoospermic patients with FSH values <10 IU/L as a sensitive and specific biomarker (AUC>0.88; p-value<0.006). This article is protected by copyright. All rights reserved. Keywords: Seminal plasma; azoospermia; extracellular vesicles; miRNA; non-invasive biomarker.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/188943
url https://hdl.handle.net/2445/188943
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv versió postprint del document publicat a: https://doi.org/10.1111/andr.13286
Andrology, 2022
https://doi.org/10.1111/andr.13286
dc.rights.none.fl_str_mv (c) American Society of Andrology and European Academy of Andrology, 2022
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) American Society of Andrology and European Academy of Andrology, 2022
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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