Plant Defenses Against Pests Driven by a Bidirectional Promoter

16 Pág.

Bibliographic Details
Authors: Arnaiz, Ana, Martínez, Manuel, Gonzalez-Melendi, P., Grbić, Vojislava, Díaz, Isabel, Santamaria, M. Estrella
Format: article
Status:Published version
Publication Date:2019
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/368810
Online Access:http://hdl.handle.net/10261/368810
https://api.elsevier.com/content/abstract/scopus_id/85069537565
Access Level:Open access
Keyword:Pieris brassicae
Tetranychus urticae
Bidirectional promoter
Cis-elements
Defense genes
Inducible promoter
Reporter gene expression
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spelling Plant Defenses Against Pests Driven by a Bidirectional PromoterArnaiz, AnaMartínez, ManuelGonzalez-Melendi, P.Grbić, VojislavaDíaz, IsabelSantamaria, M. EstrellaPieris brassicaeTetranychus urticaeBidirectional promoterCis-elementsDefense genesInducible promoterReporter gene expression16 Pág.The plant defense responses to pests results in the synchronized change of a complex network of interconnected genes and signaling pathways. An essential part of this process is mediated by the binding of transcription factors to the specific responsive cis-elements within in the promoters of phytophagous-responsive genes. In this work, it is reported the identification and characterization of a bidirectional promoter that simultaneously co-regulate two divergent genes, At5g10300 and At5g10290, upon arthropod feeding. Computational analysis identified the presence of cis-elements within the intergenic region between two loci, mainly from the DOF but also from the AP2/ERF, Golden 2-like and bHLH families. The function of the bidirectional promoter was analyzed using two enhanced variants of the GFP and CherryFP reporter genes, in both orientations, in transient tobacco and stably transformed Arabidopsis plants. Promoter activity was tested in response to feeding of Tetranychus urticae and Pieris brassicae, as well as wounding, flagellin and chitin treatments. Using RT-qPCR assays and confocal microscopy, it was shown that all treatments resulted in the induction of both reporter genes. Furthermore, our findings revealed the asymmetric character of the promoter with stronger activity in the forward than in the reverse orientation. This study provides an example of a bidirectional promoter with a strong potential to be used in plant biotechnology in pest control that requires stacking of the defense genes.This work was supported by the project BIO2017-83472-R from the Ministerio de Ciencia y Universidades of Spain. A Ph.D. Studentship from the Universidad Politecnica de Madrid (UPM) financed AA, and a Ramon y Cajal Grant (RYC-2017-21814) financed MES.Peer reviewedFrontiers MediaMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Universidad Politécnica de MadridArnaiz, Ana [0000-0002-3842-498X]Martínez, Manuel [0000-0002-7826-5872]Gonzalez-Melendi, P. [0000-0003-4355-4495]Grbić, Vojislava [0000-0001-6570-8796]Díaz, Isabel [0000-0001-9865-902X]Santamaria, M. Estrella [0000-0003-4999-6227]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/368810https://api.elsevier.com/content/abstract/scopus_id/85069537565reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-83472-RCentro de Biotecnología y Genómica de Plantas (CBGP)https://doi.org/10.3389/fpls.2019.00930Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3688102026-05-22T06:33:51Z
dc.title.none.fl_str_mv Plant Defenses Against Pests Driven by a Bidirectional Promoter
title Plant Defenses Against Pests Driven by a Bidirectional Promoter
spellingShingle Plant Defenses Against Pests Driven by a Bidirectional Promoter
Arnaiz, Ana
Pieris brassicae
Tetranychus urticae
Bidirectional promoter
Cis-elements
Defense genes
Inducible promoter
Reporter gene expression
title_short Plant Defenses Against Pests Driven by a Bidirectional Promoter
title_full Plant Defenses Against Pests Driven by a Bidirectional Promoter
title_fullStr Plant Defenses Against Pests Driven by a Bidirectional Promoter
title_full_unstemmed Plant Defenses Against Pests Driven by a Bidirectional Promoter
title_sort Plant Defenses Against Pests Driven by a Bidirectional Promoter
dc.creator.none.fl_str_mv Arnaiz, Ana
Martínez, Manuel
Gonzalez-Melendi, P.
Grbić, Vojislava
Díaz, Isabel
Santamaria, M. Estrella
author Arnaiz, Ana
author_facet Arnaiz, Ana
Martínez, Manuel
Gonzalez-Melendi, P.
Grbić, Vojislava
Díaz, Isabel
Santamaria, M. Estrella
author_role author
author2 Martínez, Manuel
Gonzalez-Melendi, P.
Grbić, Vojislava
Díaz, Isabel
Santamaria, M. Estrella
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Universidad Politécnica de Madrid
Arnaiz, Ana [0000-0002-3842-498X]
Martínez, Manuel [0000-0002-7826-5872]
Gonzalez-Melendi, P. [0000-0003-4355-4495]
Grbić, Vojislava [0000-0001-6570-8796]
Díaz, Isabel [0000-0001-9865-902X]
Santamaria, M. Estrella [0000-0003-4999-6227]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Pieris brassicae
Tetranychus urticae
Bidirectional promoter
Cis-elements
Defense genes
Inducible promoter
Reporter gene expression
topic Pieris brassicae
Tetranychus urticae
Bidirectional promoter
Cis-elements
Defense genes
Inducible promoter
Reporter gene expression
description 16 Pág.
publishDate 2019
dc.date.none.fl_str_mv 2019
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/368810
https://api.elsevier.com/content/abstract/scopus_id/85069537565
url http://hdl.handle.net/10261/368810
https://api.elsevier.com/content/abstract/scopus_id/85069537565
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-83472-R
Centro de Biotecnología y Genómica de Plantas (CBGP)
https://doi.org/10.3389/fpls.2019.00930
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869406840676155392
score 15.811543

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