The Gap Junction Inhibitor Octanol Decreases Proliferation and Increases Glial Differentiation of Postnatal Neural Progenitor Cells

Neural precursor cells (NPCs) that persist in the postnatal/adult subventricular zone (SVZ) express connexins that form hemichannels and gap junctions. Gap junctional communication plays a role in NPC proliferation and differentiation during development, but its relevance on postnatal age remains to...

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Autores: Talaverón Aguilocho, Rocío, Morado Díaz, Camilo José, Herrera Delgado, Alejandro, Gálvez, Victoria, Pastor Loro, Ángel Manuel, Rodríguez Matarredona, Esperanza
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Recursos:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/161348
Acesso em linha:https://hdl.handle.net/11441/161348
https://doi.org/10.3390/ijms25126288
Access Level:acceso abierto
Palavra-chave:Connexin
Differentiation
Glioblastoma
Neural stem cells
Proliferation
Subventricular zone
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spelling The Gap Junction Inhibitor Octanol Decreases Proliferation and Increases Glial Differentiation of Postnatal Neural Progenitor CellsTalaverón Aguilocho, RocíoMorado Díaz, Camilo JoséHerrera Delgado, AlejandroGálvez, VictoriaPastor Loro, Ángel ManuelRodríguez Matarredona, EsperanzaConnexinDifferentiationGlioblastomaNeural stem cellsProliferationSubventricular zoneNeural precursor cells (NPCs) that persist in the postnatal/adult subventricular zone (SVZ) express connexins that form hemichannels and gap junctions. Gap junctional communication plays a role in NPC proliferation and differentiation during development, but its relevance on postnatal age remains to be elucidated. In this work we aimed to evaluate the effect of the blockade of gap junctional communication on proliferation and cell fate of NPCs obtained from the SVZ of postnatal rats. NPCs were isolated and expanded in culture as neurospheres. Electron microscopy revealed the existence of gap junctions among neurosphere cells. Treatment of cultures with octanol, a broad-spectrum gap junction blocker, or with Gap27, a specific blocker for gap junctions formed by connexin43, produced a significant decrease in bromodeoxyuridine incorporation. Octanol treatment also exerted a dose-dependent antiproliferative effect on glioblastoma cells. To analyze possible actions on NPC fate, cells were seeded in the absence of mitogens. Treatment with octanol led to an increase in the percentage of astrocytes and oligodendrocyte precursors, whereas the percentage of neurons remained unchanged. Gap27 treatment, in contrast, did not modify the differentiation pattern of SVZ NPCs. Our results indicate that general blockade of gap junctions with octanol induces significant effects on the behavior of postnatal SVZ NPCs, by reducing proliferation and promoting glial differentiation.Ministerio de Ciencia e Innovación PGC2018-094654-B-I00, PID2021-124300NBI00Junta de Andalucía P20_00529Premio Mensual Publicación Científica Destacada de la US. Facultad de BiologíaMultidisciplinary Digital Publishing Institute (MDPI)FisiologíaMicrobiologíaMinisterio de Ciencia e Innovación (MICIN). EspañaJunta de Andalucía2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/161348https://doi.org/10.3390/ijms25126288reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésInternational Journal of Molecular Sciences, 25 (12), 6288.PGC2018-094654-B-I00P20_00529https://doi.org/10.3390/ijms25126288info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1613482026-06-17T12:51:07Z
dc.title.none.fl_str_mv The Gap Junction Inhibitor Octanol Decreases Proliferation and Increases Glial Differentiation of Postnatal Neural Progenitor Cells
title The Gap Junction Inhibitor Octanol Decreases Proliferation and Increases Glial Differentiation of Postnatal Neural Progenitor Cells
spellingShingle The Gap Junction Inhibitor Octanol Decreases Proliferation and Increases Glial Differentiation of Postnatal Neural Progenitor Cells
Talaverón Aguilocho, Rocío
Connexin
Differentiation
Glioblastoma
Neural stem cells
Proliferation
Subventricular zone
title_short The Gap Junction Inhibitor Octanol Decreases Proliferation and Increases Glial Differentiation of Postnatal Neural Progenitor Cells
title_full The Gap Junction Inhibitor Octanol Decreases Proliferation and Increases Glial Differentiation of Postnatal Neural Progenitor Cells
title_fullStr The Gap Junction Inhibitor Octanol Decreases Proliferation and Increases Glial Differentiation of Postnatal Neural Progenitor Cells
title_full_unstemmed The Gap Junction Inhibitor Octanol Decreases Proliferation and Increases Glial Differentiation of Postnatal Neural Progenitor Cells
title_sort The Gap Junction Inhibitor Octanol Decreases Proliferation and Increases Glial Differentiation of Postnatal Neural Progenitor Cells
dc.creator.none.fl_str_mv Talaverón Aguilocho, Rocío
Morado Díaz, Camilo José
Herrera Delgado, Alejandro
Gálvez, Victoria
Pastor Loro, Ángel Manuel
Rodríguez Matarredona, Esperanza
author Talaverón Aguilocho, Rocío
author_facet Talaverón Aguilocho, Rocío
Morado Díaz, Camilo José
Herrera Delgado, Alejandro
Gálvez, Victoria
Pastor Loro, Ángel Manuel
Rodríguez Matarredona, Esperanza
author_role author
author2 Morado Díaz, Camilo José
Herrera Delgado, Alejandro
Gálvez, Victoria
Pastor Loro, Ángel Manuel
Rodríguez Matarredona, Esperanza
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Fisiología
Microbiología
Ministerio de Ciencia e Innovación (MICIN). España
Junta de Andalucía
dc.subject.none.fl_str_mv Connexin
Differentiation
Glioblastoma
Neural stem cells
Proliferation
Subventricular zone
topic Connexin
Differentiation
Glioblastoma
Neural stem cells
Proliferation
Subventricular zone
description Neural precursor cells (NPCs) that persist in the postnatal/adult subventricular zone (SVZ) express connexins that form hemichannels and gap junctions. Gap junctional communication plays a role in NPC proliferation and differentiation during development, but its relevance on postnatal age remains to be elucidated. In this work we aimed to evaluate the effect of the blockade of gap junctional communication on proliferation and cell fate of NPCs obtained from the SVZ of postnatal rats. NPCs were isolated and expanded in culture as neurospheres. Electron microscopy revealed the existence of gap junctions among neurosphere cells. Treatment of cultures with octanol, a broad-spectrum gap junction blocker, or with Gap27, a specific blocker for gap junctions formed by connexin43, produced a significant decrease in bromodeoxyuridine incorporation. Octanol treatment also exerted a dose-dependent antiproliferative effect on glioblastoma cells. To analyze possible actions on NPC fate, cells were seeded in the absence of mitogens. Treatment with octanol led to an increase in the percentage of astrocytes and oligodendrocyte precursors, whereas the percentage of neurons remained unchanged. Gap27 treatment, in contrast, did not modify the differentiation pattern of SVZ NPCs. Our results indicate that general blockade of gap junctions with octanol induces significant effects on the behavior of postnatal SVZ NPCs, by reducing proliferation and promoting glial differentiation.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/161348
https://doi.org/10.3390/ijms25126288
url https://hdl.handle.net/11441/161348
https://doi.org/10.3390/ijms25126288
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv International Journal of Molecular Sciences, 25 (12), 6288.
PGC2018-094654-B-I00
P20_00529
https://doi.org/10.3390/ijms25126288
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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