The wiring diagram of antennal lobe and mapping a brain circuit that controls chemotaxis behavior in the Drosophila larva
Drosophila larvae present unique opportunity for anatomical and functional mapping of their nervous system because of features such as numerical simplicity of neurons its nervous system is composed of, and ability to exhibit quantifiable behaviors such as chemotaxis. Here, we mapped entire antennal...
| Autor: | |
|---|---|
| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2017 |
| País: | España |
| Institución: | CBUC, CESCA |
| Repositorio: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/663806 |
| Acceso en línea: | http://hdl.handle.net/10803/663806 |
| Access Level: | acceso abierto |
| Palabra clave: | Chemotaxis Antennal lobe Drosophila Electron Microscopy Connectome Quimiotaxis Lóbulo antennal Microscopia electronica Conectoma 616.8 |
| Sumario: | Drosophila larvae present unique opportunity for anatomical and functional mapping of their nervous system because of features such as numerical simplicity of neurons its nervous system is composed of, and ability to exhibit quantifiable behaviors such as chemotaxis. Here, we mapped entire antennal lobe of larval Drosophila with one of its circuits responsible for controlling sensorimotor transformation in lateral horn (LH) (higher brain) through a single brain descending neuron using electron microscopic 3D reconstruction. In antennal lobe, we reported a canonical circuit with uniglomerular projection neurons (uPNs), working to relay gain-controlled ORN activity to higher brain centers like Mushroom body and lateral horn. We also found a parallel circuit with multiglomerular projection neurons (mPNs) and hierarchically organized local neurons (LNs) selectively integrating signal from multiple ORNs at the first synapse with LN-LN connectivity putatively implementing gain control mechanism that can potentially switch from computing distinguished odor signals through panglomerular inhibition to allowing system to respond to faint aversive odor in an environment rich with strong appetitive odors. We also reconstructed and studied one of the olfactory connected circuits in the LH that was found to be influencing chemotaxis behavior in larva through a single brain descending neuron, PVM027. We found that this neuron was responsible in controlling stop response of chemotaxis behavior. EM reconstruction revealed its connection with variety of motor systems and SEZ descending neurons in the VNC. Connections were revealed with the peristaltic wave propagation circuit of larva, and PVM027 was found to be implementing stop by terminating and ceasing the origin of forward peristaltic waves. |
|---|