Quantitative Assessment of Hormogonia Induction in Nostoc punctiforme by a Fluorescent Reporter Strain

While symbiotic plant–cyanobacteria interactions hold significant potential for revolutionizing agricultural practices by reducing the application of artificial nitrogen fertilizers, the genetic underpinnings of the symbiotic interaction between the plant host and the cyanobiont remain poorly unders...

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Detalhes bibliográficos
Autores: Neubauer, Anna, Iniesta Pallarés, Macarena, Álvarez Núñez, Consolación, Bailly, Aurélien, Szövényi, Péter, Mariscal, Vicente
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2025
País:España
Recursos:Universidad de Sevilla (US)
Repositório:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/175162
Acesso em linha:https://hdl.handle.net/11441/175162
https://doi.org/10.1093/jxb/eraf197
Access Level:Acceso aberto
Palavra-chave:Anthoceros agrestis
Biological Nitrogen fixation
Cyanobiont
Hormogonia
Nostoc punctiforme
Plant–cyanobacteria symbiosis
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spelling Quantitative Assessment of Hormogonia Induction in Nostoc punctiforme by a Fluorescent Reporter StrainNeubauer, AnnaIniesta Pallarés, MacarenaÁlvarez Núñez, ConsolaciónBailly, AurélienSzövényi, PéterMariscal, VicenteAnthoceros agrestisBiological Nitrogen fixationCyanobiontHormogoniaNostoc punctiformePlant–cyanobacteria symbiosisWhile symbiotic plant–cyanobacteria interactions hold significant potential for revolutionizing agricultural practices by reducing the application of artificial nitrogen fertilizers, the genetic underpinnings of the symbiotic interaction between the plant host and the cyanobiont remain poorly understood. In particular, the molecular mechanisms through which host plants induce the formation of motile cyanobacterial filaments (hormogonia), essential for colonization and initiation of symbiosis, are not well characterized. In this study, we present a novel yet objective method for quantifying hormogonia induction, addressing limitations of traditional qualitative approaches. We have developed a reporter strain of Nostoc punctiforme PCC 73102 capable of quantifying hormogonia induction in response to diverse biotic and abiotic stimuli. This reporter strain, generated via triparental mating conjugation transformation, contains the promoter sequence of prepilin pilA fused to a green fluorescent protein (GFP) and enables quantitative and high-throughput monitoring of hormogonia induction using a microplate reader. Our innovative approach, employing a cyanobacterial hormogonia reporter strain, allows high-throughput screening of the hormogonia-inducing effect of a wide array of environmental and plant signals. This method is expected to greatly advance our understanding of the genetic determinants underpinning plant–cyanobacteria symbioses.Oxford University PressBioquímica Vegetal y Biología MolecularMinisterio de Ciencia, Innovación y Universidades (MICIU). EspañaUniversidad de Sevilla2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/175162https://doi.org/10.1093/jxb/eraf197reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésJournal of Experimental Botany.PID2023-153052OB-I00VI PPIT-UShttps://doi.org/10.1093/jxb/eraf197info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1751622026-06-17T12:51:07Z
dc.title.none.fl_str_mv Quantitative Assessment of Hormogonia Induction in Nostoc punctiforme by a Fluorescent Reporter Strain
title Quantitative Assessment of Hormogonia Induction in Nostoc punctiforme by a Fluorescent Reporter Strain
spellingShingle Quantitative Assessment of Hormogonia Induction in Nostoc punctiforme by a Fluorescent Reporter Strain
Neubauer, Anna
Anthoceros agrestis
Biological Nitrogen fixation
Cyanobiont
Hormogonia
Nostoc punctiforme
Plant–cyanobacteria symbiosis
title_short Quantitative Assessment of Hormogonia Induction in Nostoc punctiforme by a Fluorescent Reporter Strain
title_full Quantitative Assessment of Hormogonia Induction in Nostoc punctiforme by a Fluorescent Reporter Strain
title_fullStr Quantitative Assessment of Hormogonia Induction in Nostoc punctiforme by a Fluorescent Reporter Strain
title_full_unstemmed Quantitative Assessment of Hormogonia Induction in Nostoc punctiforme by a Fluorescent Reporter Strain
title_sort Quantitative Assessment of Hormogonia Induction in Nostoc punctiforme by a Fluorescent Reporter Strain
dc.creator.none.fl_str_mv Neubauer, Anna
Iniesta Pallarés, Macarena
Álvarez Núñez, Consolación
Bailly, Aurélien
Szövényi, Péter
Mariscal, Vicente
author Neubauer, Anna
author_facet Neubauer, Anna
Iniesta Pallarés, Macarena
Álvarez Núñez, Consolación
Bailly, Aurélien
Szövényi, Péter
Mariscal, Vicente
author_role author
author2 Iniesta Pallarés, Macarena
Álvarez Núñez, Consolación
Bailly, Aurélien
Szövényi, Péter
Mariscal, Vicente
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Bioquímica Vegetal y Biología Molecular
Ministerio de Ciencia, Innovación y Universidades (MICIU). España
Universidad de Sevilla
dc.subject.none.fl_str_mv Anthoceros agrestis
Biological Nitrogen fixation
Cyanobiont
Hormogonia
Nostoc punctiforme
Plant–cyanobacteria symbiosis
topic Anthoceros agrestis
Biological Nitrogen fixation
Cyanobiont
Hormogonia
Nostoc punctiforme
Plant–cyanobacteria symbiosis
description While symbiotic plant–cyanobacteria interactions hold significant potential for revolutionizing agricultural practices by reducing the application of artificial nitrogen fertilizers, the genetic underpinnings of the symbiotic interaction between the plant host and the cyanobiont remain poorly understood. In particular, the molecular mechanisms through which host plants induce the formation of motile cyanobacterial filaments (hormogonia), essential for colonization and initiation of symbiosis, are not well characterized. In this study, we present a novel yet objective method for quantifying hormogonia induction, addressing limitations of traditional qualitative approaches. We have developed a reporter strain of Nostoc punctiforme PCC 73102 capable of quantifying hormogonia induction in response to diverse biotic and abiotic stimuli. This reporter strain, generated via triparental mating conjugation transformation, contains the promoter sequence of prepilin pilA fused to a green fluorescent protein (GFP) and enables quantitative and high-throughput monitoring of hormogonia induction using a microplate reader. Our innovative approach, employing a cyanobacterial hormogonia reporter strain, allows high-throughput screening of the hormogonia-inducing effect of a wide array of environmental and plant signals. This method is expected to greatly advance our understanding of the genetic determinants underpinning plant–cyanobacteria symbioses.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/175162
https://doi.org/10.1093/jxb/eraf197
url https://hdl.handle.net/11441/175162
https://doi.org/10.1093/jxb/eraf197
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Journal of Experimental Botany.
PID2023-153052OB-I00
VI PPIT-US
https://doi.org/10.1093/jxb/eraf197
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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