Genetic variation between Spanish and American versions of sweet corn inbred lines

Conservation of maize inbred lines in different stations causes variability among strains. The objective of this research was to determine agronomic and molecular differences in American sweet corn inbreds maintained in Spain. American and Spanish strains of five sweet corn inbred lines were charact...

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Detalles Bibliográficos
Autores: Revilla Temiño, Pedro, Abuín, María del Carmen, Malvar Pintos, Rosa Ana, Soengas, Pilar, Ordás López, Bernardo, Ordás Pérez, Amando
Tipo de recurso: artículo
Estado:Versión enviada para evaluación y publicación
Fecha de publicación:2005
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/101765
Acceso en línea:http://hdl.handle.net/10261/101765
Access Level:acceso abierto
Palabra clave:Zea mays
Germplasm conservation
Variability
RAPD
Descripción
Sumario:Conservation of maize inbred lines in different stations causes variability among strains. The objective of this research was to determine agronomic and molecular differences in American sweet corn inbreds maintained in Spain. American and Spanish strains of five sweet corn inbred lines were characterized by using 34 RAPD primers that produced 168 consistent bands. Strains of four of these inbreds were crossed in a diallel design, and hybrids were evaluated in four environments in northwestern Spain. The RAPD characterization showed no differences between strains for two inbreds, while divergence between strains was largest for the inbred 15125. Most primers did not reveal any variability between pairs of strains, while some primers produced variations at high rates. Differences in agronomic performance among Spanish and American strains were most important for P51, followed by P39, while strains 1453 or 15125 were not significantly different. Molecular differences between strains were not related to agronomic performance. Residual heterozygosity or outcrossing cannot explain these results. Lack of adaptation could have caused stress-induced mutagenesis. Natural selection could have eliminated unfavourable selective mutations, but neutral mutations can be found at the molecular level and favourable mutations could have been selected at the agronomic level.