Bacillus subtilis RadA/Sms-Mediated Nascent Lagging-Strand Unwinding at Stalled or Reversed Forks Is a Two-Step Process: RadA/Sms Assists RecA Nucleation, and RecA Loads RadA/Sms

The supporting information can be downloaded at: https://www. mdpi.com/article/10.3390/ijms24054536/s1

Detalles Bibliográficos
Autores: Torres, Rubén, Carrasco, Begoña, Alonso, Juan C
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/362768
Acceso en línea:http://hdl.handle.net/10261/362768
https://api.elsevier.com/content/abstract/scopus_id/85149859370
Access Level:acceso abierto
Palabra clave:RadA/Sms-RecA interaction
RecO
SsbA
branch migration translocases
id ES_40d73342f48ee18296cd00ea539cf0ee
oai_identifier_str oai:digital.csic.es:10261/362768
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spelling Bacillus subtilis RadA/Sms-Mediated Nascent Lagging-Strand Unwinding at Stalled or Reversed Forks Is a Two-Step Process: RadA/Sms Assists RecA Nucleation, and RecA Loads RadA/SmsTorres, RubénCarrasco, BegoñaAlonso, Juan CRadA/Sms-RecA interactionRecOSsbAbranch migration translocasesThe supporting information can be downloaded at: https://www. mdpi.com/article/10.3390/ijms24054536/s1Replication fork rescue requires Bacillus subtilis RecA, its negative (SsbA) and positive (RecO) mediators, and fork-processing (RadA/Sms). To understand how they work to promote fork remodeling, reconstituted branched replication intermediates were used. We show that RadA/Sms (or its variant, RadA/Sms C13A) binds to the 5'-tail of a reversed fork with longer nascent lagging-strand and unwinds it in the 5'→3' direction, but RecA and its mediators limit unwinding. RadA/Sms cannot unwind a reversed fork with a longer nascent leading-strand, or a gapped stalled fork, but RecA interacts with and activates unwinding. Here, the molecular mechanism by which RadA/Sms, in concert with RecA, in a two-step reaction, unwinds the nascent lagging-strand of reversed or stalled forks is unveiled. First, RadA/Sms, as a mediator, contributes to SsbA displacement from the forks and nucleates RecA onto single-stranded DNA. Then, RecA, as a loader, interacts with and recruits RadA/Sms onto the nascent lagging strand of these DNA substrates to unwind them. Within this process, RecA limits RadA/Sms self-assembly to control fork processing, and RadA/Sms prevents RecA from provoking unnecessary recombination.This research was funded by the Ministerio de Ciencia e Innovación (MCIN)/Agencia Estatal de Investigación (AEI)/10.13039/501100011033/FEDER, EU (PID2021-122273NB-I00) and by the CSIC (2021AEP031) to J.C.APeer reviewedMolecular Diversity Preservation InternationalMinisterio de Ciencia e Innovación (España)Torres, Rubén [0000-0002-3665-5426]Carrasco, Begoña [0000-0002-5671-1296]Alonso, Juan C [0000-0002-5178-7179]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/362768https://api.elsevier.com/content/abstract/scopus_id/85149859370reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-122273NB-I00International journal of molecular sciencesapplication/pdfhttps://www.mdpi.com/1422-0067/24/5/4536Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3627682026-05-22T06:33:51Z
dc.title.none.fl_str_mv Bacillus subtilis RadA/Sms-Mediated Nascent Lagging-Strand Unwinding at Stalled or Reversed Forks Is a Two-Step Process: RadA/Sms Assists RecA Nucleation, and RecA Loads RadA/Sms
title Bacillus subtilis RadA/Sms-Mediated Nascent Lagging-Strand Unwinding at Stalled or Reversed Forks Is a Two-Step Process: RadA/Sms Assists RecA Nucleation, and RecA Loads RadA/Sms
spellingShingle Bacillus subtilis RadA/Sms-Mediated Nascent Lagging-Strand Unwinding at Stalled or Reversed Forks Is a Two-Step Process: RadA/Sms Assists RecA Nucleation, and RecA Loads RadA/Sms
Torres, Rubén
RadA/Sms-RecA interaction
RecO
SsbA
branch migration translocases
title_short Bacillus subtilis RadA/Sms-Mediated Nascent Lagging-Strand Unwinding at Stalled or Reversed Forks Is a Two-Step Process: RadA/Sms Assists RecA Nucleation, and RecA Loads RadA/Sms
title_full Bacillus subtilis RadA/Sms-Mediated Nascent Lagging-Strand Unwinding at Stalled or Reversed Forks Is a Two-Step Process: RadA/Sms Assists RecA Nucleation, and RecA Loads RadA/Sms
title_fullStr Bacillus subtilis RadA/Sms-Mediated Nascent Lagging-Strand Unwinding at Stalled or Reversed Forks Is a Two-Step Process: RadA/Sms Assists RecA Nucleation, and RecA Loads RadA/Sms
title_full_unstemmed Bacillus subtilis RadA/Sms-Mediated Nascent Lagging-Strand Unwinding at Stalled or Reversed Forks Is a Two-Step Process: RadA/Sms Assists RecA Nucleation, and RecA Loads RadA/Sms
title_sort Bacillus subtilis RadA/Sms-Mediated Nascent Lagging-Strand Unwinding at Stalled or Reversed Forks Is a Two-Step Process: RadA/Sms Assists RecA Nucleation, and RecA Loads RadA/Sms
dc.creator.none.fl_str_mv Torres, Rubén
Carrasco, Begoña
Alonso, Juan C
author Torres, Rubén
author_facet Torres, Rubén
Carrasco, Begoña
Alonso, Juan C
author_role author
author2 Carrasco, Begoña
Alonso, Juan C
author2_role author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Torres, Rubén [0000-0002-3665-5426]
Carrasco, Begoña [0000-0002-5671-1296]
Alonso, Juan C [0000-0002-5178-7179]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv RadA/Sms-RecA interaction
RecO
SsbA
branch migration translocases
topic RadA/Sms-RecA interaction
RecO
SsbA
branch migration translocases
description The supporting information can be downloaded at: https://www. mdpi.com/article/10.3390/ijms24054536/s1
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/362768
https://api.elsevier.com/content/abstract/scopus_id/85149859370
url http://hdl.handle.net/10261/362768
https://api.elsevier.com/content/abstract/scopus_id/85149859370
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-122273NB-I00
International journal of molecular sciences
application/pdf
https://www.mdpi.com/1422-0067/24/5/4536
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812455

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