Bioavailability and Metabolic Fate of (Poly)phenols from Hull-Less Purple Whole-Grain Barley in Humans

Background and Objectives: Anthocyanin-rich barley varieties have recently gained attention due to their high (poly)phenolic content and potential health benefits, yet human data on their bioavailability remain scarce. This study aimed to characterize the absorption, metabolism, and excretion of (po...

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Autores: Cortijo-Alfonso, Maria Engracia, Yuste, Silvia, Martínez Subirà, Mariona, Moralejo Vidal, Mª Angeles, Piñol Felis, Carme, Macià i Puig, Ma Alba, Rubió Piqué, Laura
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Recursos:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/469011
Acesso em linha:https://doi.org/10.3390/nu17193086
https://hdl.handle.net/10459.1/469011
Access Level:acceso abierto
Palavra-chave:Barley (poly)phenols
Bioavailability
Metabolism
Postprandial study
UPLCMS/MS
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spelling Bioavailability and Metabolic Fate of (Poly)phenols from Hull-Less Purple Whole-Grain Barley in HumansCortijo-Alfonso, Maria EngraciaYuste, SilviaMartínez Subirà, MarionaMoralejo Vidal, Mª AngelesPiñol Felis, CarmeMacià i Puig, Ma AlbaRubió Piqué, LauraBarley (poly)phenolsBioavailabilityMetabolismPostprandial studyUPLCMS/MSBackground and Objectives: Anthocyanin-rich barley varieties have recently gained attention due to their high (poly)phenolic content and potential health benefits, yet human data on their bioavailability remain scarce. This study aimed to characterize the absorption, metabolism, and excretion of (poly)phenolic compounds from a novel hull-less purple whole-grain barley (WGB) genotype. Methods: Eleven healthy volunteers consumed 140 g of purple WGB biscuits, and plasma and urine samples were collected over 6 h and 48 h, respectively. Results: UPLC-MS/MS analysis revealed a broad range of metabolites, with 11 (poly)phenolic compounds identified in plasma and 80 in urine. The biscuits were particularly rich in flavones (217 mg/140 g, mainly chrysoeriol derivatives), followed by hydroxycinnamic acids (~54 mg, mainly 4′-hydroxy-3′-methoxycinnamic acid), anthocyanins (44.8 mg), and flavan-3-ols (16.8 mg). In plasma, glycosylated anthocyanins and flavone conjugates (e.g., peonidin-3-O-glucuronide, chrysoeriol-O-glucuronide) were detectable within 1–2 h, consistent with early absorption. In contrast, microbial-derived catabolites—including valerolactones, phenylacetic and benzoic acids—were mainly excreted in urine between 8 and 24 h, reaching concentrations above 1000 nM. Conclusions: These findings provide novel insights into the bioavailability and metabolic fate of barley (poly)phenols, supporting their potential contribution to host and gut health. As a proof-of-concept study, it complements the limited data available from pigmented cereals and underscores the need for validation in larger cohorts.This work was funded by the Spanish Ministry of Industry, Economy and Competitiveness, the Agencia Estatal de Investigación (AEI) and the European Regional Development Fund (ERDF) through the PID2020-113009RB-100 INNOBAR project, as well as by the Generalitat de Catalunya through the Maria Engracia Cortijo predoctoral FI SDUR grant. Silvia Yuste was supported by a Margarita Salas postdoctoral fellowship, funded by the European Union—NextGeneration EU through the Ministerio de Universidades and the Universitat de Lleida. The APC was funded by University of Lleida.MDPI2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.3390/nu17193086https://hdl.handle.net/10459.1/469011reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)Inglésinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113009RB-I00Reproducció del document publicat a https://doi.org/10.3390/nu17193086Nutrients, 2025, vol. 17, núm. 19cc-by, (c) Cortijo-Alfonso et al., 2025Attribution 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:repositori.udl.cat:10459.1/4690112026-06-24T12:42:17Z
dc.title.none.fl_str_mv Bioavailability and Metabolic Fate of (Poly)phenols from Hull-Less Purple Whole-Grain Barley in Humans
title Bioavailability and Metabolic Fate of (Poly)phenols from Hull-Less Purple Whole-Grain Barley in Humans
spellingShingle Bioavailability and Metabolic Fate of (Poly)phenols from Hull-Less Purple Whole-Grain Barley in Humans
Cortijo-Alfonso, Maria Engracia
Barley (poly)phenols
Bioavailability
Metabolism
Postprandial study
UPLCMS/MS
title_short Bioavailability and Metabolic Fate of (Poly)phenols from Hull-Less Purple Whole-Grain Barley in Humans
title_full Bioavailability and Metabolic Fate of (Poly)phenols from Hull-Less Purple Whole-Grain Barley in Humans
title_fullStr Bioavailability and Metabolic Fate of (Poly)phenols from Hull-Less Purple Whole-Grain Barley in Humans
title_full_unstemmed Bioavailability and Metabolic Fate of (Poly)phenols from Hull-Less Purple Whole-Grain Barley in Humans
title_sort Bioavailability and Metabolic Fate of (Poly)phenols from Hull-Less Purple Whole-Grain Barley in Humans
dc.creator.none.fl_str_mv Cortijo-Alfonso, Maria Engracia
Yuste, Silvia
Martínez Subirà, Mariona
Moralejo Vidal, Mª Angeles
Piñol Felis, Carme
Macià i Puig, Ma Alba
Rubió Piqué, Laura
author Cortijo-Alfonso, Maria Engracia
author_facet Cortijo-Alfonso, Maria Engracia
Yuste, Silvia
Martínez Subirà, Mariona
Moralejo Vidal, Mª Angeles
Piñol Felis, Carme
Macià i Puig, Ma Alba
Rubió Piqué, Laura
author_role author
author2 Yuste, Silvia
Martínez Subirà, Mariona
Moralejo Vidal, Mª Angeles
Piñol Felis, Carme
Macià i Puig, Ma Alba
Rubió Piqué, Laura
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Barley (poly)phenols
Bioavailability
Metabolism
Postprandial study
UPLCMS/MS
topic Barley (poly)phenols
Bioavailability
Metabolism
Postprandial study
UPLCMS/MS
description Background and Objectives: Anthocyanin-rich barley varieties have recently gained attention due to their high (poly)phenolic content and potential health benefits, yet human data on their bioavailability remain scarce. This study aimed to characterize the absorption, metabolism, and excretion of (poly)phenolic compounds from a novel hull-less purple whole-grain barley (WGB) genotype. Methods: Eleven healthy volunteers consumed 140 g of purple WGB biscuits, and plasma and urine samples were collected over 6 h and 48 h, respectively. Results: UPLC-MS/MS analysis revealed a broad range of metabolites, with 11 (poly)phenolic compounds identified in plasma and 80 in urine. The biscuits were particularly rich in flavones (217 mg/140 g, mainly chrysoeriol derivatives), followed by hydroxycinnamic acids (~54 mg, mainly 4′-hydroxy-3′-methoxycinnamic acid), anthocyanins (44.8 mg), and flavan-3-ols (16.8 mg). In plasma, glycosylated anthocyanins and flavone conjugates (e.g., peonidin-3-O-glucuronide, chrysoeriol-O-glucuronide) were detectable within 1–2 h, consistent with early absorption. In contrast, microbial-derived catabolites—including valerolactones, phenylacetic and benzoic acids—were mainly excreted in urine between 8 and 24 h, reaching concentrations above 1000 nM. Conclusions: These findings provide novel insights into the bioavailability and metabolic fate of barley (poly)phenols, supporting their potential contribution to host and gut health. As a proof-of-concept study, it complements the limited data available from pigmented cereals and underscores the need for validation in larger cohorts.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.3390/nu17193086
https://hdl.handle.net/10459.1/469011
url https://doi.org/10.3390/nu17193086
https://hdl.handle.net/10459.1/469011
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113009RB-I00
Reproducció del document publicat a https://doi.org/10.3390/nu17193086
Nutrients, 2025, vol. 17, núm. 19
dc.rights.none.fl_str_mv cc-by, (c) Cortijo-Alfonso et al., 2025
Attribution 4.0 International
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by, (c) Cortijo-Alfonso et al., 2025
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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