DATASET_The role of Penicillium expansum histone deacetylases HosA and HosB in growth, development, and patulin production

This dataset is made available under a CC-BY license (https://creativecommons.org/licenses/by/4.0/)

Detalles Bibliográficos
Autores: Llobregat, Belén, Abad Fuentes, Antonio, Mercader Badia, Josep Vicent, González-Candelas, Luis, Ballester Frutos, Ana Rosa
Tipo de recurso: conjunto de datos
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/405434
Acceso en línea:http://hdl.handle.net/10261/405434
https://doi.org/10.20350/digitalCSIC/17707
Access Level:acceso abierto
Palabra clave:Epigenetics
Histone deacetylases
Gene expression
Mycotoxins
Penicillium expansum
mycotoxins
gene expression
epigenetics
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oai_identifier_str oai:digital.csic.es:10261/405434
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv DATASET_The role of Penicillium expansum histone deacetylases HosA and HosB in growth, development, and patulin production
title DATASET_The role of Penicillium expansum histone deacetylases HosA and HosB in growth, development, and patulin production
spellingShingle DATASET_The role of Penicillium expansum histone deacetylases HosA and HosB in growth, development, and patulin production
Llobregat, Belén
Epigenetics
Histone deacetylases
Gene expression
Mycotoxins
Penicillium expansum
Penicillium expansum
mycotoxins
gene expression
epigenetics
title_short DATASET_The role of Penicillium expansum histone deacetylases HosA and HosB in growth, development, and patulin production
title_full DATASET_The role of Penicillium expansum histone deacetylases HosA and HosB in growth, development, and patulin production
title_fullStr DATASET_The role of Penicillium expansum histone deacetylases HosA and HosB in growth, development, and patulin production
title_full_unstemmed DATASET_The role of Penicillium expansum histone deacetylases HosA and HosB in growth, development, and patulin production
title_sort DATASET_The role of Penicillium expansum histone deacetylases HosA and HosB in growth, development, and patulin production
dc.creator.none.fl_str_mv Llobregat, Belén
Abad Fuentes, Antonio
Mercader Badia, Josep Vicent
González-Candelas, Luis
Ballester Frutos, Ana Rosa
author Llobregat, Belén
author_facet Llobregat, Belén
Abad Fuentes, Antonio
Mercader Badia, Josep Vicent
González-Candelas, Luis
Ballester Frutos, Ana Rosa
author_role author
author2 Abad Fuentes, Antonio
Mercader Badia, Josep Vicent
González-Candelas, Luis
Ballester Frutos, Ana Rosa
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
Generalitat Valenciana
European Commission
Consejo Superior de Investigaciones Científicas (España)
Llobregat, Belén [0000-0002-4480-572X]
González-Candelas, Luis [0000-0002-2579-6880]
Ballester Frutos, Ana Rosa [0000-0002-7688-7838]
Ballester Frutos, Ana Rosa [anarosa.ballester@iata.csic.es]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Epigenetics
Histone deacetylases
Gene expression
Mycotoxins
Penicillium expansum
Penicillium expansum
mycotoxins
gene expression
epigenetics
topic Epigenetics
Histone deacetylases
Gene expression
Mycotoxins
Penicillium expansum
Penicillium expansum
mycotoxins
gene expression
epigenetics
description This dataset is made available under a CC-BY license (https://creativecommons.org/licenses/by/4.0/)
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
http://purl.org/coar/resource_type/c_ddb1
format dataset
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/405434
https://doi.org/10.20350/digitalCSIC/17707
url http://hdl.handle.net/10261/405434
https://doi.org/10.20350/digitalCSIC/17707
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093392-A-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-126005OB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/CEX2021-001189-S
Llobregat, B.; Abad-Fuentes, A.; Mercader, J. V.; González-Candelas, L.; Ballester, A.-R. The role of Penicillium expansum histone deacetylases HosA and HosB in growth, development, and patulin production. http://doi.org/10.1016/j.micres.2025.128181. http://hdl.handle.net/10261/387886
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/zip
dc.publisher.none.fl_str_mv DIGITAL.CSIC
publisher.none.fl_str_mv DIGITAL.CSIC
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
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spelling DATASET_The role of Penicillium expansum histone deacetylases HosA and HosB in growth, development, and patulin productionLlobregat, BelénAbad Fuentes, AntonioMercader Badia, Josep VicentGonzález-Candelas, LuisBallester Frutos, Ana RosaEpigeneticsHistone deacetylasesGene expressionMycotoxinsPenicillium expansumPenicillium expansummycotoxinsgene expressionepigeneticsThis dataset is made available under a CC-BY license (https://creativecommons.org/licenses/by/4.0/)Histone modifications are key epigenetic mechanisms for gene regulation in response to environmental stimuli. Histone acetylation is crucial for regulating chromatin accessibility and is controlled by histone-modifying enzymes: histone acetyltransferases (HATs) and histone deacetylases (HDACs). This study examined the roles of two HDACs, HosA and HosB, in the fungus Penicillium expansum. While the deletion of hosB did not affect the phenotype, HosA was found to play a crucial role in growth, development, and conidiation. The ΔhosA strain exhibited a characteristic fluffy phenotype and a significant reduction in conidiation. Expression analysis indicated that these differences were related to lower expression of the core regulatory gene wetA, and, to a lesser extent, brlA and abaA. Additionally, the growth of ΔhosA was negatively affected by the addition of calcofluor white and sodium chloride, while the deletion of hosA increased tolerance to sodium dodecyl sulfate and hydrogen peroxide on solid media. Furthermore, the ΔhosA strain showed an abnormal pattern of patulin production during in vitro growth, and reduced virulence likely due to growth retardation and impaired conidiation. These findings suggest that HosA is an epigenetic regulator of conidiation and plays an indirect role in secondary metabolite production and virulence in P. expansum.This work was partially funded by projects RTI2018–093392-A-I00, PID2021–126005OBI00 (MCIN/AEI/10.13039/501100011033/FEDER, UE), AGROALNEXT/2022/028 (GVA, PRTR, MRR, NextGenerationEU), and PIE 202270I070 (CSIC). The Accreditation as Center of Excellence Severo Ochoa CEX2021–001189-S funded by MCIN/AEI/10.13039/501100011033 is also fully acknowledged. B.L. was a recipient of the predoctoral contract PRE2019–089326 (MICINN/AEI/FSE, UE). The authors acknowledge the support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI).With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2021-001189-S).Figure_1: Bioinformatic analysis of putative class I and class II HDAC proteins in different fungi -- Figure_2: Phenotypic traits of the wild-type strain of A. carbonarius ITEM 5010, two ectopic mutants and three knockout mutants of genes otaA and veA, denoted as Dpks and DveA, respectively. -- Figure_3: Phenotypic characteristics of the different strains growing under different stress conditions. -- Figure_4: In vitro patulin production. -- Figure_5: RT-qPCR analysis of the gene expression patterns. -- Figure_6: Infection of ‘Golden Delicious’ apples by P. expansum strain CMP-1, DhosA, and DhosB knockout mutants, and complemented hosAC and hosBC strains.Peer reviewedDIGITAL.CSICAgencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)Generalitat ValencianaEuropean CommissionConsejo Superior de Investigaciones Científicas (España)Llobregat, Belén [0000-0002-4480-572X]González-Candelas, Luis [0000-0002-2579-6880]Ballester Frutos, Ana Rosa [0000-0002-7688-7838]Ballester Frutos, Ana Rosa [anarosa.ballester@iata.csic.es]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/datasethttp://purl.org/coar/resource_type/c_ddb1application/ziphttp://hdl.handle.net/10261/405434https://doi.org/10.20350/digitalCSIC/17707reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093392-A-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-126005OB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/CEX2021-001189-SLlobregat, B.; Abad-Fuentes, A.; Mercader, J. V.; González-Candelas, L.; Ballester, A.-R. The role of Penicillium expansum histone deacetylases HosA and HosB in growth, development, and patulin production. http://doi.org/10.1016/j.micres.2025.128181. http://hdl.handle.net/10261/387886Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4054342026-05-22T06:33:51Z
score 15,81155

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