Identification of target genes to control acetate yield during aerobic fermentation with Saccharomyces cerevisiae

Background: Aerobic fermentation of grape must, leading to respiro-fermentative metabolism of sugars, has been proposed as way of reducing alcohol content in wines. Two factors limit the usefulness of Saccharomyces cerevisiae for this application, the Crabtree effect, and excess volatile acidity und...

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Autores: Curiel, J.A. [0000-0002-0111-1437], Salvadó, Z. [0000-0003-4412-1421], Tronchoni, J. [0000-0001-9227-2713], Morales, P., Rodrigues, A.J., Quirós, M., Gonzalez, R.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Universidad de La Rioja (UR)
Repositorio:RIUR. Repositorio Institucional de la Universidad de La Rioja
OAI Identifier:oai:portal.dialnet.es:doc/5bbc687db750603269e809df
Acceso en línea:https://investigacion.unirioja.es/documentos/5bbc687db750603269e809df
Access Level:acceso abierto
Palabra clave:Aerobic fermentation
Alcohol level reduction
Carbon catabolite derepression
Crabtree effect
Volatile acidity
Wine
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spelling Identification of target genes to control acetate yield during aerobic fermentation with Saccharomyces cerevisiaeCuriel, J.A. [0000-0002-0111-1437]Salvadó, Z. [0000-0003-4412-1421]Tronchoni, J. [0000-0001-9227-2713]Morales, P.Rodrigues, A.J.Quirós, M.Gonzalez, R.Aerobic fermentationAlcohol level reductionCarbon catabolite derepressionCrabtree effectVolatile acidityWineBackground: Aerobic fermentation of grape must, leading to respiro-fermentative metabolism of sugars, has been proposed as way of reducing alcohol content in wines. Two factors limit the usefulness of Saccharomyces cerevisiae for this application, the Crabtree effect, and excess volatile acidity under aerobic conditions. This work aimed to explore the impact on ethanol acetate production of different S. cerevisiae strains deleted for genes previously related with the Crabtree phenotype. Results: Recombinant strains were constructed on a wine industrial genetic background, FX10. All yeast strains, including FX10, showed respiro-fermentative metabolism in natural grape must under aerobic conditions, as well as a concomitant reduction in ethanol yield. This indicates that the Crabtree effect is not a major constrain for reaching relevant respiration levels in grape must. Indeed, only minor differences in ethanol yield were observed between the original and some of the recombinant strains. In contrast, some yeast strains showed a relevant reduction of acetic acid production. This was identified as a positive feature for the feasibility of alcohol level reduction by respiration. Reduced acetic acid production was confirmed by a thorough analysis of these and some additional deletion strains (involving genes HXK2, PYK1, REG1, PDE2 and PDC1). Some recombinant yeasts showed altered production of glycerol and pyruvate derived metabolites. Conclusions:REG1 and PDC1 deletion strains showed a strong reduction of acetic acid yield in aerobic fermentations. Since REG1 defective strains may be obtained by non-GMO approaches, these gene modifications show good promise to help reducing ethanol content in wines. © 2016 The Author(s).2016info:eu-repo/semantics/articleSubtype: Articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://investigacion.unirioja.es/documentos/5bbc687db750603269e809dfreponame:RIUR. Repositorio Institucional de la Universidad de La Riojainstname:Universidad de La Rioja (UR)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1186/S12934-016-0555-Yinfo:eu-repo/semantics/altIdentifier/wos/WOS:000383760300001info:eu-repo/semantics/altIdentifier/pissn/1475-2859Identification of target genes to control acetate yield during aerobic fermentation with Saccharomyces cerevisiae, 2016, vol. 15, núm. 1info:eu-repo/semantics/openAccessoai:portal.dialnet.es:doc/5bbc687db750603269e809df2026-06-14T12:47:17Z
dc.title.none.fl_str_mv Identification of target genes to control acetate yield during aerobic fermentation with Saccharomyces cerevisiae
title Identification of target genes to control acetate yield during aerobic fermentation with Saccharomyces cerevisiae
spellingShingle Identification of target genes to control acetate yield during aerobic fermentation with Saccharomyces cerevisiae
Curiel, J.A. [0000-0002-0111-1437]
Aerobic fermentation
Alcohol level reduction
Carbon catabolite derepression
Crabtree effect
Volatile acidity
Wine
title_short Identification of target genes to control acetate yield during aerobic fermentation with Saccharomyces cerevisiae
title_full Identification of target genes to control acetate yield during aerobic fermentation with Saccharomyces cerevisiae
title_fullStr Identification of target genes to control acetate yield during aerobic fermentation with Saccharomyces cerevisiae
title_full_unstemmed Identification of target genes to control acetate yield during aerobic fermentation with Saccharomyces cerevisiae
title_sort Identification of target genes to control acetate yield during aerobic fermentation with Saccharomyces cerevisiae
dc.creator.none.fl_str_mv Curiel, J.A. [0000-0002-0111-1437]
Salvadó, Z. [0000-0003-4412-1421]
Tronchoni, J. [0000-0001-9227-2713]
Morales, P.
Rodrigues, A.J.
Quirós, M.
Gonzalez, R.
author Curiel, J.A. [0000-0002-0111-1437]
author_facet Curiel, J.A. [0000-0002-0111-1437]
Salvadó, Z. [0000-0003-4412-1421]
Tronchoni, J. [0000-0001-9227-2713]
Morales, P.
Rodrigues, A.J.
Quirós, M.
Gonzalez, R.
author_role author
author2 Salvadó, Z. [0000-0003-4412-1421]
Tronchoni, J. [0000-0001-9227-2713]
Morales, P.
Rodrigues, A.J.
Quirós, M.
Gonzalez, R.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Aerobic fermentation
Alcohol level reduction
Carbon catabolite derepression
Crabtree effect
Volatile acidity
Wine
topic Aerobic fermentation
Alcohol level reduction
Carbon catabolite derepression
Crabtree effect
Volatile acidity
Wine
description Background: Aerobic fermentation of grape must, leading to respiro-fermentative metabolism of sugars, has been proposed as way of reducing alcohol content in wines. Two factors limit the usefulness of Saccharomyces cerevisiae for this application, the Crabtree effect, and excess volatile acidity under aerobic conditions. This work aimed to explore the impact on ethanol acetate production of different S. cerevisiae strains deleted for genes previously related with the Crabtree phenotype. Results: Recombinant strains were constructed on a wine industrial genetic background, FX10. All yeast strains, including FX10, showed respiro-fermentative metabolism in natural grape must under aerobic conditions, as well as a concomitant reduction in ethanol yield. This indicates that the Crabtree effect is not a major constrain for reaching relevant respiration levels in grape must. Indeed, only minor differences in ethanol yield were observed between the original and some of the recombinant strains. In contrast, some yeast strains showed a relevant reduction of acetic acid production. This was identified as a positive feature for the feasibility of alcohol level reduction by respiration. Reduced acetic acid production was confirmed by a thorough analysis of these and some additional deletion strains (involving genes HXK2, PYK1, REG1, PDE2 and PDC1). Some recombinant yeasts showed altered production of glycerol and pyruvate derived metabolites. Conclusions:REG1 and PDC1 deletion strains showed a strong reduction of acetic acid yield in aerobic fermentations. Since REG1 defective strains may be obtained by non-GMO approaches, these gene modifications show good promise to help reducing ethanol content in wines. © 2016 The Author(s).
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
Subtype: Article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://investigacion.unirioja.es/documentos/5bbc687db750603269e809df
url https://investigacion.unirioja.es/documentos/5bbc687db750603269e809df
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/S12934-016-0555-Y
info:eu-repo/semantics/altIdentifier/wos/WOS:000383760300001
info:eu-repo/semantics/altIdentifier/pissn/1475-2859
Identification of target genes to control acetate yield during aerobic fermentation with Saccharomyces cerevisiae, 2016, vol. 15, núm. 1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:RIUR. Repositorio Institucional de la Universidad de La Rioja
instname:Universidad de La Rioja (UR)
instname_str Universidad de La Rioja (UR)
reponame_str RIUR. Repositorio Institucional de la Universidad de La Rioja
collection RIUR. Repositorio Institucional de la Universidad de La Rioja
repository.name.fl_str_mv
repository.mail.fl_str_mv
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