The heterocyst differentiation transcriptional regulator HetR of the filamentous cyanobactrium Anabaena forms tetramers and can be regulated by phosphorylation

Many filamentous cyanobacteria respond to the external cue of nitrogen scarcity by the differentiation of heterocysts, cells specialized in the fixation of atmospheric nitrogen in oxic environments. Heterocysts follow a spatial pattern along the filament of two heterocysts separated by ca. 10–15 veg...

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Detalles Bibliográficos
Autores: Valladares, Ana, Flores, Enrique, Herrero, Antonia
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/147433
Acceso en línea:http://hdl.handle.net/10261/147433
Access Level:acceso abierto
Palabra clave:Heterocyst differentiation
Post-translational modification
Anabaena
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repository_id_str
spelling The heterocyst differentiation transcriptional regulator HetR of the filamentous cyanobactrium Anabaena forms tetramers and can be regulated by phosphorylationValladares, AnaFlores, EnriqueHerrero, AntoniaHeterocyst differentiationPost-translational modificationAnabaenaMany filamentous cyanobacteria respond to the external cue of nitrogen scarcity by the differentiation of heterocysts, cells specialized in the fixation of atmospheric nitrogen in oxic environments. Heterocysts follow a spatial pattern along the filament of two heterocysts separated by ca. 10–15 vegetative cells performing oxygenic photosynthesis. HetR is a transcriptional regulator that directs heterocyst differentiation. In the model strain Anabaena sp. PCC 7120, the HetR protein was observed in various oligomeric forms in vivo, including a tetramer that peaked with maximal hetR expression during differentiation. Tetramers were not detected in a hetR point mutant incapable of differentiation, but were conspicuous in an over-differentiating strain lacking the PatS inhibitor. In differentiated filaments the HetR tetramer was restricted to heterocysts, being undetectable in vegetative cells. HetR co-purified with RNA polymerase from Anabaena mainly as a tetramer. In vitro, purified recombinant HetR was distributed between monomers, dimers, trimers and tetramers, and it was phosphorylated when incubated with (γ-32P)ATP. Phosphorylation and PatS hampered the accumulation of HetR tetramers and impaired HetR binding to DNA. In summary, tetrameric HetR appears to represent a functionally relevant form of HetR, whose abundance in the Anabaena filament could be negatively regulated by phosphorylation and by PatS.Peer ReviewedBlackwell PublishingConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2017201720162017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/147433reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1474332026-05-22T06:33:51Z
dc.title.none.fl_str_mv The heterocyst differentiation transcriptional regulator HetR of the filamentous cyanobactrium Anabaena forms tetramers and can be regulated by phosphorylation
title The heterocyst differentiation transcriptional regulator HetR of the filamentous cyanobactrium Anabaena forms tetramers and can be regulated by phosphorylation
spellingShingle The heterocyst differentiation transcriptional regulator HetR of the filamentous cyanobactrium Anabaena forms tetramers and can be regulated by phosphorylation
Valladares, Ana
Heterocyst differentiation
Post-translational modification
Anabaena
title_short The heterocyst differentiation transcriptional regulator HetR of the filamentous cyanobactrium Anabaena forms tetramers and can be regulated by phosphorylation
title_full The heterocyst differentiation transcriptional regulator HetR of the filamentous cyanobactrium Anabaena forms tetramers and can be regulated by phosphorylation
title_fullStr The heterocyst differentiation transcriptional regulator HetR of the filamentous cyanobactrium Anabaena forms tetramers and can be regulated by phosphorylation
title_full_unstemmed The heterocyst differentiation transcriptional regulator HetR of the filamentous cyanobactrium Anabaena forms tetramers and can be regulated by phosphorylation
title_sort The heterocyst differentiation transcriptional regulator HetR of the filamentous cyanobactrium Anabaena forms tetramers and can be regulated by phosphorylation
dc.creator.none.fl_str_mv Valladares, Ana
Flores, Enrique
Herrero, Antonia
author Valladares, Ana
author_facet Valladares, Ana
Flores, Enrique
Herrero, Antonia
author_role author
author2 Flores, Enrique
Herrero, Antonia
author2_role author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Heterocyst differentiation
Post-translational modification
Anabaena
topic Heterocyst differentiation
Post-translational modification
Anabaena
description Many filamentous cyanobacteria respond to the external cue of nitrogen scarcity by the differentiation of heterocysts, cells specialized in the fixation of atmospheric nitrogen in oxic environments. Heterocysts follow a spatial pattern along the filament of two heterocysts separated by ca. 10–15 vegetative cells performing oxygenic photosynthesis. HetR is a transcriptional regulator that directs heterocyst differentiation. In the model strain Anabaena sp. PCC 7120, the HetR protein was observed in various oligomeric forms in vivo, including a tetramer that peaked with maximal hetR expression during differentiation. Tetramers were not detected in a hetR point mutant incapable of differentiation, but were conspicuous in an over-differentiating strain lacking the PatS inhibitor. In differentiated filaments the HetR tetramer was restricted to heterocysts, being undetectable in vegetative cells. HetR co-purified with RNA polymerase from Anabaena mainly as a tetramer. In vitro, purified recombinant HetR was distributed between monomers, dimers, trimers and tetramers, and it was phosphorylated when incubated with (γ-32P)ATP. Phosphorylation and PatS hampered the accumulation of HetR tetramers and impaired HetR binding to DNA. In summary, tetrameric HetR appears to represent a functionally relevant form of HetR, whose abundance in the Anabaena filament could be negatively regulated by phosphorylation and by PatS.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/147433
url http://hdl.handle.net/10261/147433
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Blackwell Publishing
publisher.none.fl_str_mv Blackwell Publishing
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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