Local administration of porcine immunomodulatory, chemotactic and angiogenic extracellular vesicles using engineered cardiac scaffolds for myocardial infarction

The administration of extracellular vesicles (EV) from mesenchymal stromal cells (MSC) is a promising cell-free nanotherapy for tissue repair after myocardial infarction (MI). However, the optimal EV delivery strategy remains undetermined. Here, we designed a novel MSC-EV delivery, using 3D scaffold...

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Autores: Monguió-Tortajada, Marta|||0000-0003-2125-0810, Prat-Vidal, Cristina|||0000-0002-5621-1101, Font-Morón, Miriam|||0000-0002-4714-252X, Clos Sansalvador, Marta|||0000-0001-8522-7818, Calle Arias, Alexandra|||0000-0003-3903-5019, Gastelurrutia, Paloma|||0000-0001-6974-9210, Cserkóová, Adriana, Morancho, Anna|||0000-0001-9059-3206, Ramírez de Paz, Miguel Ángel|||0000-0002-5868-2134, Rosell Novel, Anna|||0000-0003-1082-3599, Bayés-Genís, Antoni|||0000-0002-3044-197X, Gálvez-Montón, Carolina|||0000-0003-2254-9371, Borràs i Serres, Francesc Enric|||0000-0003-4038-1912, Rudilla, F..
Formato: artículo
Fecha de publicación:2021
País:España
Recursos:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:238253
Acesso em linha:https://ddd.uab.cat/record/238253
https://dx.doi.org/urn:doi:10.1016/j.bioactmat.2021.02.026
Access Level:acceso abierto
Palavra-chave:Exosomes
Mesenchymal stem/stromal cells
Migration
Infiltration
Cardiac tissue engineering
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oai_identifier_str oai:ddd.uab.cat:238253
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network_name_str España
repository_id_str
dc.title.none.fl_str_mv Local administration of porcine immunomodulatory, chemotactic and angiogenic extracellular vesicles using engineered cardiac scaffolds for myocardial infarction
title Local administration of porcine immunomodulatory, chemotactic and angiogenic extracellular vesicles using engineered cardiac scaffolds for myocardial infarction
spellingShingle Local administration of porcine immunomodulatory, chemotactic and angiogenic extracellular vesicles using engineered cardiac scaffolds for myocardial infarction
Monguió-Tortajada, Marta|||0000-0003-2125-0810
Exosomes
Mesenchymal stem/stromal cells
Migration
Infiltration
Cardiac tissue engineering
title_short Local administration of porcine immunomodulatory, chemotactic and angiogenic extracellular vesicles using engineered cardiac scaffolds for myocardial infarction
title_full Local administration of porcine immunomodulatory, chemotactic and angiogenic extracellular vesicles using engineered cardiac scaffolds for myocardial infarction
title_fullStr Local administration of porcine immunomodulatory, chemotactic and angiogenic extracellular vesicles using engineered cardiac scaffolds for myocardial infarction
title_full_unstemmed Local administration of porcine immunomodulatory, chemotactic and angiogenic extracellular vesicles using engineered cardiac scaffolds for myocardial infarction
title_sort Local administration of porcine immunomodulatory, chemotactic and angiogenic extracellular vesicles using engineered cardiac scaffolds for myocardial infarction
dc.creator.none.fl_str_mv Monguió-Tortajada, Marta|||0000-0003-2125-0810
Prat-Vidal, Cristina|||0000-0002-5621-1101
Font-Morón, Miriam|||0000-0002-4714-252X
Clos Sansalvador, Marta|||0000-0001-8522-7818
Calle Arias, Alexandra|||0000-0003-3903-5019
Gastelurrutia, Paloma|||0000-0001-6974-9210
Cserkóová, Adriana
Morancho, Anna|||0000-0001-9059-3206
Ramírez de Paz, Miguel Ángel|||0000-0002-5868-2134
Rosell Novel, Anna|||0000-0003-1082-3599
Bayés-Genís, Antoni|||0000-0002-3044-197X
Gálvez-Montón, Carolina|||0000-0003-2254-9371
Borràs i Serres, Francesc Enric|||0000-0003-4038-1912
Rudilla, F..
author Monguió-Tortajada, Marta|||0000-0003-2125-0810
author_facet Monguió-Tortajada, Marta|||0000-0003-2125-0810
Prat-Vidal, Cristina|||0000-0002-5621-1101
Font-Morón, Miriam|||0000-0002-4714-252X
Clos Sansalvador, Marta|||0000-0001-8522-7818
Calle Arias, Alexandra|||0000-0003-3903-5019
Gastelurrutia, Paloma|||0000-0001-6974-9210
Cserkóová, Adriana
Morancho, Anna|||0000-0001-9059-3206
Ramírez de Paz, Miguel Ángel|||0000-0002-5868-2134
Rosell Novel, Anna|||0000-0003-1082-3599
Bayés-Genís, Antoni|||0000-0002-3044-197X
Gálvez-Montón, Carolina|||0000-0003-2254-9371
Borràs i Serres, Francesc Enric|||0000-0003-4038-1912
Rudilla, F..
author_role author
author2 Prat-Vidal, Cristina|||0000-0002-5621-1101
Font-Morón, Miriam|||0000-0002-4714-252X
Clos Sansalvador, Marta|||0000-0001-8522-7818
Calle Arias, Alexandra|||0000-0003-3903-5019
Gastelurrutia, Paloma|||0000-0001-6974-9210
Cserkóová, Adriana
Morancho, Anna|||0000-0001-9059-3206
Ramírez de Paz, Miguel Ángel|||0000-0002-5868-2134
Rosell Novel, Anna|||0000-0003-1082-3599
Bayés-Genís, Antoni|||0000-0002-3044-197X
Gálvez-Montón, Carolina|||0000-0003-2254-9371
Borràs i Serres, Francesc Enric|||0000-0003-4038-1912
Rudilla, F..
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Exosomes
Mesenchymal stem/stromal cells
Migration
Infiltration
Cardiac tissue engineering
topic Exosomes
Mesenchymal stem/stromal cells
Migration
Infiltration
Cardiac tissue engineering
description The administration of extracellular vesicles (EV) from mesenchymal stromal cells (MSC) is a promising cell-free nanotherapy for tissue repair after myocardial infarction (MI). However, the optimal EV delivery strategy remains undetermined. Here, we designed a novel MSC-EV delivery, using 3D scaffolds engineered from decellularised cardiac tissue as a cell-free product for cardiac repair. EV from porcine cardiac adipose tissue-derived MSC (cATMSC) were purified by size exclusion chromatography (SEC), functionally analysed and loaded to scaffolds. cATMSC-EV markedly reduced polyclonal proliferation and pro-inflammatory cytokines production (IFNγ, TNFα, IL12p40) of allogeneic PBMC. Moreover, cATMSC-EV recruited outgrowth endothelial cells (OEC) and allogeneic MSC, and promoted angiogenesis. Fluorescently labelled cATMSC-EV were mixed with peptide hydrogel, and were successfully retained in decellularised scaffolds. Then, cATMSC-EV-embedded pericardial scaffolds were administered in vivo over the ischemic myocardium in a pig model of MI. Six days from implantation, the engineered scaffold efficiently integrated into the post-infarcted myocardium. cATMSC-EV were detected within the construct and MI core, and promoted an increase in vascular density and reduction in macrophage and T cell infiltration within the damaged myocardium. The confined administration of multifunctional MSC-EV within an engineered pericardial scaffold ensures local EV dosage and release, and generates a vascularised bioactive niche for cell recruitment, engraftment and modulation of short-term post-ischemic inflammation.
publishDate 2021
dc.date.none.fl_str_mv 2
2021-01-01
2021
2021-01-01
dc.type.none.fl_str_mv Article
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https://dx.doi.org/urn:doi:10.1016/j.bioactmat.2021.02.026
url https://ddd.uab.cat/record/238253
https://dx.doi.org/urn:doi:10.1016/j.bioactmat.2021.02.026
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-301
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Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-483
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Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI18-01227
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Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 RD16-0009
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Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2019-PROD-00122
Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 CPII15-00003
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dc.rights.none.fl_str_mv open access
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spelling Local administration of porcine immunomodulatory, chemotactic and angiogenic extracellular vesicles using engineered cardiac scaffolds for myocardial infarctionMonguió-Tortajada, Marta|||0000-0003-2125-0810Prat-Vidal, Cristina|||0000-0002-5621-1101Font-Morón, Miriam|||0000-0002-4714-252XClos Sansalvador, Marta|||0000-0001-8522-7818Calle Arias, Alexandra|||0000-0003-3903-5019Gastelurrutia, Paloma|||0000-0001-6974-9210Cserkóová, AdrianaMorancho, Anna|||0000-0001-9059-3206Ramírez de Paz, Miguel Ángel|||0000-0002-5868-2134Rosell Novel, Anna|||0000-0003-1082-3599Bayés-Genís, Antoni|||0000-0002-3044-197XGálvez-Montón, Carolina|||0000-0003-2254-9371Borràs i Serres, Francesc Enric|||0000-0003-4038-1912Rudilla, F..ExosomesMesenchymal stem/stromal cellsMigrationInfiltrationCardiac tissue engineeringThe administration of extracellular vesicles (EV) from mesenchymal stromal cells (MSC) is a promising cell-free nanotherapy for tissue repair after myocardial infarction (MI). However, the optimal EV delivery strategy remains undetermined. Here, we designed a novel MSC-EV delivery, using 3D scaffolds engineered from decellularised cardiac tissue as a cell-free product for cardiac repair. EV from porcine cardiac adipose tissue-derived MSC (cATMSC) were purified by size exclusion chromatography (SEC), functionally analysed and loaded to scaffolds. cATMSC-EV markedly reduced polyclonal proliferation and pro-inflammatory cytokines production (IFNγ, TNFα, IL12p40) of allogeneic PBMC. Moreover, cATMSC-EV recruited outgrowth endothelial cells (OEC) and allogeneic MSC, and promoted angiogenesis. Fluorescently labelled cATMSC-EV were mixed with peptide hydrogel, and were successfully retained in decellularised scaffolds. Then, cATMSC-EV-embedded pericardial scaffolds were administered in vivo over the ischemic myocardium in a pig model of MI. Six days from implantation, the engineered scaffold efficiently integrated into the post-infarcted myocardium. cATMSC-EV were detected within the construct and MI core, and promoted an increase in vascular density and reduction in macrophage and T cell infiltration within the damaged myocardium. The confined administration of multifunctional MSC-EV within an engineered pericardial scaffold ensures local EV dosage and release, and generates a vascularised bioactive niche for cell recruitment, engraftment and modulation of short-term post-ischemic inflammation. 22021-01-0120212021-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/238253https://dx.doi.org/urn:doi:10.1016/j.bioactmat.2021.02.026reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-301Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-1427Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-483Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 SAF2017-84324-C2-1-RAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-110137RB-I00Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-107145RB-I00Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI17-01487Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PIC18-00014Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 ICI19-00039Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI18-00256Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI18-01227Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 ICI20-00135Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 RD16-0009Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 RD16-0011-0006Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 CB16-11-00403Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2019-PROD-00122Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 CPII15-00003Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI16-00981open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2382532026-06-06T12:50:31Z
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