Thermomechanical response of a representative porin for biomimetics
The thermomechanical response of Omp2a, a representative porin used for the fabrication of smart biomimetic nanomembranes, has been characterized using microcantilever technology and compared with standard proteins. For this purpose, thermally induced transitions involving the conversion of stable t...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Universitat Politècnica de Catalunya (UPC) |
| Repositorio: | UPCommons. Portal del coneixement obert de la UPC |
| Idioma: | inglés |
| OAI Identifier: | oai:upcommons.upc.edu:2117/130343 |
| Acceso en línea: | https://hdl.handle.net/2117/130343 https://dx.doi.org/10.1021/acsomega.8b00463 |
| Access Level: | acceso abierto |
| Palabra clave: | Proteins Molecular dynamics Albumins Mechanical properties Thermal properties Proteïnes Dinàmica molecular Àrees temàtiques de la UPC::Enginyeria química |
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Thermomechanical response of a representative porin for biomimeticsLopes Rodrigues, Maximilien|||0000-0002-9405-336XPuiggalí Jou, Anna|||0000-0002-2234-9436Martí Ballesté, DidacValle Mendoza, Luis Javier del|||0000-0001-9916-1741Michaux, Catherine Anne GisèlePerpète, Eric A.Alemán Llansó, Carlos|||0000-0003-4462-6075ProteinsMolecular dynamicsAlbuminsMechanical propertiesMolecular dynamicsProteinsThermal propertiesProteïnesDinàmica molecularÀrees temàtiques de la UPC::Enginyeria químicaThe thermomechanical response of Omp2a, a representative porin used for the fabrication of smart biomimetic nanomembranes, has been characterized using microcantilever technology and compared with standard proteins. For this purpose, thermally induced transitions involving the conversion of stable trimers to bigger aggregates, local reorganizations based on the strengthening or weakening of intermolecular interactions, and protein denaturation have been detected by the microcantilever resonance frequency and deflection as a function of the temperature. Measurements have been carried out on arrays of 8-microcantilevers functionalized with proteins (Omp2a, lysozyme and bovine serum albumin). To interpret the measured nanofeatures, the response of proteins to temperature has been also examined using other characterization techniques, including real time wide angle X-ray diffraction. Results not only demonstrate the complex behavior of porins, which exhibit multiple local thermal transitions before undergoing denaturation at temperatures higher than 105 °C, but also suggest a posttreatment to control the orientation of immobilized Omp2a molecules in functionalized biomimetic nanomembranes and, thus, increase their efficacy in ion transport.Peer ReviewedAmerican Chemical Society (ACS)20182018-07-3120192019-03-13journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/130343https://dx.doi.org/10.1021/acsomega.8b00463reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivs 3.0 Spainhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/1303432026-05-27T15:37:01Z |
| dc.title.none.fl_str_mv |
Thermomechanical response of a representative porin for biomimetics |
| title |
Thermomechanical response of a representative porin for biomimetics |
| spellingShingle |
Thermomechanical response of a representative porin for biomimetics Lopes Rodrigues, Maximilien|||0000-0002-9405-336X Proteins Molecular dynamics Albumins Mechanical properties Molecular dynamics Proteins Thermal properties Proteïnes Dinàmica molecular Àrees temàtiques de la UPC::Enginyeria química |
| title_short |
Thermomechanical response of a representative porin for biomimetics |
| title_full |
Thermomechanical response of a representative porin for biomimetics |
| title_fullStr |
Thermomechanical response of a representative porin for biomimetics |
| title_full_unstemmed |
Thermomechanical response of a representative porin for biomimetics |
| title_sort |
Thermomechanical response of a representative porin for biomimetics |
| dc.creator.none.fl_str_mv |
Lopes Rodrigues, Maximilien|||0000-0002-9405-336X Puiggalí Jou, Anna|||0000-0002-2234-9436 Martí Ballesté, Didac Valle Mendoza, Luis Javier del|||0000-0001-9916-1741 Michaux, Catherine Anne Gisèle Perpète, Eric A. Alemán Llansó, Carlos|||0000-0003-4462-6075 |
| author |
Lopes Rodrigues, Maximilien|||0000-0002-9405-336X |
| author_facet |
Lopes Rodrigues, Maximilien|||0000-0002-9405-336X Puiggalí Jou, Anna|||0000-0002-2234-9436 Martí Ballesté, Didac Valle Mendoza, Luis Javier del|||0000-0001-9916-1741 Michaux, Catherine Anne Gisèle Perpète, Eric A. Alemán Llansó, Carlos|||0000-0003-4462-6075 |
| author_role |
author |
| author2 |
Puiggalí Jou, Anna|||0000-0002-2234-9436 Martí Ballesté, Didac Valle Mendoza, Luis Javier del|||0000-0001-9916-1741 Michaux, Catherine Anne Gisèle Perpète, Eric A. Alemán Llansó, Carlos|||0000-0003-4462-6075 |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
Proteins Molecular dynamics Albumins Mechanical properties Molecular dynamics Proteins Thermal properties Proteïnes Dinàmica molecular Àrees temàtiques de la UPC::Enginyeria química |
| topic |
Proteins Molecular dynamics Albumins Mechanical properties Molecular dynamics Proteins Thermal properties Proteïnes Dinàmica molecular Àrees temàtiques de la UPC::Enginyeria química |
| description |
The thermomechanical response of Omp2a, a representative porin used for the fabrication of smart biomimetic nanomembranes, has been characterized using microcantilever technology and compared with standard proteins. For this purpose, thermally induced transitions involving the conversion of stable trimers to bigger aggregates, local reorganizations based on the strengthening or weakening of intermolecular interactions, and protein denaturation have been detected by the microcantilever resonance frequency and deflection as a function of the temperature. Measurements have been carried out on arrays of 8-microcantilevers functionalized with proteins (Omp2a, lysozyme and bovine serum albumin). To interpret the measured nanofeatures, the response of proteins to temperature has been also examined using other characterization techniques, including real time wide angle X-ray diffraction. Results not only demonstrate the complex behavior of porins, which exhibit multiple local thermal transitions before undergoing denaturation at temperatures higher than 105 °C, but also suggest a posttreatment to control the orientation of immobilized Omp2a molecules in functionalized biomimetic nanomembranes and, thus, increase their efficacy in ion transport. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018 2018-07-31 2019 2019-03-13 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 AM http://purl.org/coar/version/c_ab4af688f83e57aa |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2117/130343 https://dx.doi.org/10.1021/acsomega.8b00463 |
| url |
https://hdl.handle.net/2117/130343 https://dx.doi.org/10.1021/acsomega.8b00463 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivs 3.0 Spain http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivs 3.0 Spain http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
American Chemical Society (ACS) |
| publisher.none.fl_str_mv |
American Chemical Society (ACS) |
| dc.source.none.fl_str_mv |
reponame:UPCommons. Portal del coneixement obert de la UPC instname:Universitat Politècnica de Catalunya (UPC) |
| instname_str |
Universitat Politècnica de Catalunya (UPC) |
| reponame_str |
UPCommons. Portal del coneixement obert de la UPC |
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UPCommons. Portal del coneixement obert de la UPC |
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1869406704482910208 |
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15,300719 |