Melatonin, Protocatechuic Acid and Hydroxytyrosol Effects on Vitagenes System Against Alpha-Synuclein Toxicity

Preventing the abnormal assembly of α-synuclein (α-Syn) and the correct modulation of vitagenes system exercise strong neuroprotective effects. It has been reported that melatonin (MEL), protocatechuic acid (PCA) and hydroxytyrosol (HT) reduce α-Syn toxicity. Their effect on the vitagenes system of...

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Autores: Gallardo Fernández, Marta, Hornedo Ortega, Ruth, Cerezo López, Ana Belén, Troncoso González, Ana María, García Parrilla, María del Carmen
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/153769
Acceso en línea:https://hdl.handle.net/11441/153769
https://doi.org/10.1016/j.fct.2019.110817
Access Level:acceso abierto
Palabra clave:Hydroxytyrosol
Melatonin
Parkinson's disease
Protocatechuic acid
Vitagenes system
α-Synuclein
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spelling Melatonin, Protocatechuic Acid and Hydroxytyrosol Effects on Vitagenes System Against Alpha-Synuclein ToxicityGallardo Fernández, MartaHornedo Ortega, RuthCerezo López, Ana BelénTroncoso González, Ana MaríaGarcía Parrilla, María del CarmenHydroxytyrosolMelatoninParkinson's diseaseProtocatechuic acidVitagenes systemα-SynucleinPreventing the abnormal assembly of α-synuclein (α-Syn) and the correct modulation of vitagenes system exercise strong neuroprotective effects. It has been reported that melatonin (MEL), protocatechuic acid (PCA) and hydroxytyrosol (HT) reduce α-Syn toxicity. Their effect on the vitagenes system of PC12 cells have not been explored yet. These bioactive can cross the blood brain barrier (BBB). Therefore, this work aims to evaluate the inhibitory and destabilising capacities of MEL, PCA, HT, and their combinations on α-Syn kinetics and effects on vitagenes system (sirtuin-1 (SIRT-1), sirtuin-2 (SIRT-2), heme oxygenase (HO-1) and heat shock protein 70 (Hsp-70)). In vitro techniques (Thioflavin T (ThT), Transmission Electronic Microscopy (TEM), electrophoresis, MTT assay and qPCR) were used. Compounds, both individually and simultaneously were able to decrease the toxicity induced by α-Syn. Concurrently, occurrence of PCA (100 μM) +HT (100 μM) showed the highest inhibitory effect against α-Syn fibril formation and destabilisation of α-Syn fibrils (88 and 62%, respectively). Moreover, these compounds increased the expression of SIRT-2, HO-1 and Hsp70, contributing to a neuroprotective effect. In addition, the most important result is the increase on the expression of SIRT-2 caused by the combination of MEL + HT + PCA in the absence of α-Syn fibrils.Ministerio de Ciencia, Innovación y Universidades AGL2016-77505-C3-2-RElsevierNutrición y Bromatología, Toxicología y Medicina LegalMinisterio de Ciencia, Innovación y Universidades (MICINN). España2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/153769https://doi.org/10.1016/j.fct.2019.110817reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésFood and Chemical Toxicology, 134 (110817).AGL2016-77505-C3-2-Rhttps://doi.org/10.1016/j.fct.2019.110817info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1537692026-06-17T12:51:07Z
dc.title.none.fl_str_mv Melatonin, Protocatechuic Acid and Hydroxytyrosol Effects on Vitagenes System Against Alpha-Synuclein Toxicity
title Melatonin, Protocatechuic Acid and Hydroxytyrosol Effects on Vitagenes System Against Alpha-Synuclein Toxicity
spellingShingle Melatonin, Protocatechuic Acid and Hydroxytyrosol Effects on Vitagenes System Against Alpha-Synuclein Toxicity
Gallardo Fernández, Marta
Hydroxytyrosol
Melatonin
Parkinson's disease
Protocatechuic acid
Vitagenes system
α-Synuclein
title_short Melatonin, Protocatechuic Acid and Hydroxytyrosol Effects on Vitagenes System Against Alpha-Synuclein Toxicity
title_full Melatonin, Protocatechuic Acid and Hydroxytyrosol Effects on Vitagenes System Against Alpha-Synuclein Toxicity
title_fullStr Melatonin, Protocatechuic Acid and Hydroxytyrosol Effects on Vitagenes System Against Alpha-Synuclein Toxicity
title_full_unstemmed Melatonin, Protocatechuic Acid and Hydroxytyrosol Effects on Vitagenes System Against Alpha-Synuclein Toxicity
title_sort Melatonin, Protocatechuic Acid and Hydroxytyrosol Effects on Vitagenes System Against Alpha-Synuclein Toxicity
dc.creator.none.fl_str_mv Gallardo Fernández, Marta
Hornedo Ortega, Ruth
Cerezo López, Ana Belén
Troncoso González, Ana María
García Parrilla, María del Carmen
author Gallardo Fernández, Marta
author_facet Gallardo Fernández, Marta
Hornedo Ortega, Ruth
Cerezo López, Ana Belén
Troncoso González, Ana María
García Parrilla, María del Carmen
author_role author
author2 Hornedo Ortega, Ruth
Cerezo López, Ana Belén
Troncoso González, Ana María
García Parrilla, María del Carmen
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Nutrición y Bromatología, Toxicología y Medicina Legal
Ministerio de Ciencia, Innovación y Universidades (MICINN). España
dc.subject.none.fl_str_mv Hydroxytyrosol
Melatonin
Parkinson's disease
Protocatechuic acid
Vitagenes system
α-Synuclein
topic Hydroxytyrosol
Melatonin
Parkinson's disease
Protocatechuic acid
Vitagenes system
α-Synuclein
description Preventing the abnormal assembly of α-synuclein (α-Syn) and the correct modulation of vitagenes system exercise strong neuroprotective effects. It has been reported that melatonin (MEL), protocatechuic acid (PCA) and hydroxytyrosol (HT) reduce α-Syn toxicity. Their effect on the vitagenes system of PC12 cells have not been explored yet. These bioactive can cross the blood brain barrier (BBB). Therefore, this work aims to evaluate the inhibitory and destabilising capacities of MEL, PCA, HT, and their combinations on α-Syn kinetics and effects on vitagenes system (sirtuin-1 (SIRT-1), sirtuin-2 (SIRT-2), heme oxygenase (HO-1) and heat shock protein 70 (Hsp-70)). In vitro techniques (Thioflavin T (ThT), Transmission Electronic Microscopy (TEM), electrophoresis, MTT assay and qPCR) were used. Compounds, both individually and simultaneously were able to decrease the toxicity induced by α-Syn. Concurrently, occurrence of PCA (100 μM) +HT (100 μM) showed the highest inhibitory effect against α-Syn fibril formation and destabilisation of α-Syn fibrils (88 and 62%, respectively). Moreover, these compounds increased the expression of SIRT-2, HO-1 and Hsp70, contributing to a neuroprotective effect. In addition, the most important result is the increase on the expression of SIRT-2 caused by the combination of MEL + HT + PCA in the absence of α-Syn fibrils.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/153769
https://doi.org/10.1016/j.fct.2019.110817
url https://hdl.handle.net/11441/153769
https://doi.org/10.1016/j.fct.2019.110817
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Food and Chemical Toxicology, 134 (110817).
AGL2016-77505-C3-2-R
https://doi.org/10.1016/j.fct.2019.110817
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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