Surface-Bound Gradient Deposition of Protein Nanoparticles for Cell Motility Studies

A versatile evaporation-assisted methodology based on the coffee-drop effect is described to deposit nanoparticles on surfaces, obtaining for the first time patterned gradients of protein nanoparticles (pNPs) by using a simple custom-made device. Fully controllable patterns with specific periodiciti...

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Autores: Tatkiewicz, Witold I., Seras Franzoso, Joaquin, García Fruitós, Elena, Vazquez, Esther, Kyvik Ruiz, Adriana, Guasch, Judit, Villaverde, Antonio, Veciana, Jaume, Ratera, Immaculada
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/170562
Acceso en línea:http://hdl.handle.net/10261/170562
Access Level:acceso abierto
Palabra clave:Cell motility
Coffee-drop effect
Inclusion bodies
Protein nanoparticles
Surface biofunctionalization
Surface gradient deposition
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network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Surface-Bound Gradient Deposition of Protein Nanoparticles for Cell Motility Studies
title Surface-Bound Gradient Deposition of Protein Nanoparticles for Cell Motility Studies
spellingShingle Surface-Bound Gradient Deposition of Protein Nanoparticles for Cell Motility Studies
Tatkiewicz, Witold I.
Cell motility
Coffee-drop effect
Inclusion bodies
Protein nanoparticles
Surface biofunctionalization
Surface gradient deposition
title_short Surface-Bound Gradient Deposition of Protein Nanoparticles for Cell Motility Studies
title_full Surface-Bound Gradient Deposition of Protein Nanoparticles for Cell Motility Studies
title_fullStr Surface-Bound Gradient Deposition of Protein Nanoparticles for Cell Motility Studies
title_full_unstemmed Surface-Bound Gradient Deposition of Protein Nanoparticles for Cell Motility Studies
title_sort Surface-Bound Gradient Deposition of Protein Nanoparticles for Cell Motility Studies
dc.creator.none.fl_str_mv Tatkiewicz, Witold I.
Seras Franzoso, Joaquin
García Fruitós, Elena
Vazquez, Esther
Kyvik Ruiz, Adriana
Guasch, Judit
Villaverde, Antonio
Veciana, Jaume
Ratera, Immaculada
author Tatkiewicz, Witold I.
author_facet Tatkiewicz, Witold I.
Seras Franzoso, Joaquin
García Fruitós, Elena
Vazquez, Esther
Kyvik Ruiz, Adriana
Guasch, Judit
Villaverde, Antonio
Veciana, Jaume
Ratera, Immaculada
author_role author
author2 Seras Franzoso, Joaquin
García Fruitós, Elena
Vazquez, Esther
Kyvik Ruiz, Adriana
Guasch, Judit
Villaverde, Antonio
Veciana, Jaume
Ratera, Immaculada
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Dirección General de Investigación Científica y Técnica, DGICT (España)
Centro de Investigación Biomédica en Red Bioingeniería, Biomateriales y Nanomedicina (España)
Generalitat de Catalunya
European Cooperation in Science and Technology
European Commission
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Cell motility
Coffee-drop effect
Inclusion bodies
Protein nanoparticles
Surface biofunctionalization
Surface gradient deposition
topic Cell motility
Coffee-drop effect
Inclusion bodies
Protein nanoparticles
Surface biofunctionalization
Surface gradient deposition
description A versatile evaporation-assisted methodology based on the coffee-drop effect is described to deposit nanoparticles on surfaces, obtaining for the first time patterned gradients of protein nanoparticles (pNPs) by using a simple custom-made device. Fully controllable patterns with specific periodicities consisting of stripes with different widths and distinct nanoparticle concentration as well as gradients can be produced over large areas (∼10 cm2) in a fast (up to 10 mm2/min), reproducible, and cost-effective manner using an operational protocol optimized by an evolutionary algorithm. The developed method opens the possibility to decorate surfaces “a-la-carte” with pNPs enabling different categories of high-throughput studies on cell motility.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
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status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/170562
url http://hdl.handle.net/10261/170562
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MAT2016-80826-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MAT2013-50036EXP
info:eu-repo/grantAgreement/EC/H2020/654360
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SEV-2015-0496
info:eu-repo/grantAgreement/EC/FP7/600388
http://dx.doi.org/10.1021/acsami.8b06821
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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spelling Surface-Bound Gradient Deposition of Protein Nanoparticles for Cell Motility StudiesTatkiewicz, Witold I.Seras Franzoso, JoaquinGarcía Fruitós, ElenaVazquez, EstherKyvik Ruiz, AdrianaGuasch, JuditVillaverde, AntonioVeciana, JaumeRatera, ImmaculadaCell motilityCoffee-drop effectInclusion bodiesProtein nanoparticlesSurface biofunctionalizationSurface gradient depositionA versatile evaporation-assisted methodology based on the coffee-drop effect is described to deposit nanoparticles on surfaces, obtaining for the first time patterned gradients of protein nanoparticles (pNPs) by using a simple custom-made device. Fully controllable patterns with specific periodicities consisting of stripes with different widths and distinct nanoparticle concentration as well as gradients can be produced over large areas (∼10 cm2) in a fast (up to 10 mm2/min), reproducible, and cost-effective manner using an operational protocol optimized by an evolutionary algorithm. The developed method opens the possibility to decorate surfaces “a-la-carte” with pNPs enabling different categories of high-throughput studies on cell motility.The research leading to these results received funding from DGI grants (MOTHER MAT2016-80826-R and Dynamo MAT2013-50036EXP), the Networking Research Center on Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), Generalitat de Catalunya (grant 2014-SGR-17, 2017-SGR-918 and CERCA Programme), the COST Action CA15126 Between Atom and Cell, the European Social Fund and EU to J.V., A.V., and I.R. (H2020-INFRAIA-2014-2015; NFFA-654360). ICMAB acknowledges support from the Spanish Ministry of Economy and Competitiveness, through the “Severo Ochoa” Programme for Centres of Excellence in R&D (SEV-2015-0496). This work has been developed inside the Materials Science PhD programs of Universitat Autònoma de Barcelona (UAB). The authors are indebted to the “Servei de Microscòpia” of the UAB. Protein NP production has been partially performed by the ICTS “NANBIOSIS”, more specifically by the Protein Production Platform of CIBER-BBN/IBB (http://www.nanbiosis.es/unit/u1-protein-production-platform-ppp/). W.I.T. is grateful to Consejo Superior de Investigaciones Científicas (CSIC) for a “JAE-pre” fellowship. J.S.-F. is recipient of a Post-Doctoral fellowship from Asociación Española Contra el Cáncer (AECC), E.G-F. of a Post-Doctoral fellowship from INIA (DOC-INIA), and A.V. of an ICREA ACADEMIA award. This research was also supported by the People Programme (Marie Curie Actions) of the 7th Framework Programme of the European Union (FP7/2007-2013) under the grant agreement no. 600388 of REA and the Agency for Business Competitiveness ACCIÓ through a Tecniospring fellowship (TECSPR15-1-0015) to J.G. J.G. is also grateful to the Max Planck Society through the Max Planck Partner Group “Dynamic Biomimetics for Cancer Immunotherapy” in collaboration with the Max Planck Institute for Medical Research (Heidelberg, Germany).Peer reviewedAmerican Chemical SocietyDirección General de Investigación Científica y Técnica, DGICT (España)Centro de Investigación Biomédica en Red Bioingeniería, Biomateriales y Nanomedicina (España)Generalitat de CatalunyaEuropean Cooperation in Science and TechnologyEuropean CommissionMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201820182018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/170562reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MAT2016-80826-Rinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MAT2013-50036EXPinfo:eu-repo/grantAgreement/EC/H2020/654360info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SEV-2015-0496info:eu-repo/grantAgreement/EC/FP7/600388http://dx.doi.org/10.1021/acsami.8b06821Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1705622026-05-22T06:33:51Z
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