Free-labeled nanoclay intracellular uptake tracking by confocal Raman imaging
Laponite is a nanoplatform that has been successfully used as a new biomaterial for drug delivery, tissue engineering and bioimaging at the nanoscale. In general, a deep knowledge of the mechanism interaction of the nanomaterial with biological components in a physiological environment is highly des...
| Autores: | , , , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de publicación: | 2021 |
| País: | España |
| Recursos: | Universidad de Cantabria (UC) |
| Repositorio: | UCrea Repositorio Abierto de la Universidad de Cantabria |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unican.es:10902/19264 |
| Acesso em linha: | http://hdl.handle.net/10902/19264 |
| Access Level: | acceso abierto |
| Palavra-chave: | Laponite Nanomaterial Nanocarrier Raman imaging technique Nanoclay |
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Free-labeled nanoclay intracellular uptake tracking by confocal Raman imagingIturrioz Rodríguez, NereaMartín Rodríguez, RosaRenero Lecuna, CarlosAguado Menéndez, Fernando|||0000-0003-2912-0228González Legarreta, Lorena|||0000-0001-5193-7256González Gómez, Jesús Antonio|||0000-0002-0381-6393López Fanarraga, Mónica|||0000-0003-4754-311XPerdigón Aller, Ana Carmen|||0000-0003-3865-3405LaponiteNanomaterialNanocarrierRaman imaging techniqueNanoclayLaponite is a nanoplatform that has been successfully used as a new biomaterial for drug delivery, tissue engineering and bioimaging at the nanoscale. In general, a deep knowledge of the mechanism interaction of the nanomaterial with biological components in a physiological environment is highly desirable for properly characterizing its therapeutic efficacy and toxicology. Up to know, the use of fluorescent dyes labelling both, the nanomaterial and cell components, has been a requirement to characterize the cell uptake and to visualize the entrance of the nanomaterial into the cytosol and the cell nucleus. The used of fluorophores usually perturb the physiological medium and can interfere in the nanomaterial cell interaction. A new Raman imaging methodology to track the uptake and internalization of Laponite nanoparticles into J774 macrophages line cells is presented in this work. The combination of Raman spectroscopy and confocal microscopy provides direct information about the localization of the nanoparticle into the cell, through its unique vibrational fingerprint without labelling or adding dyes, and taking advantage of the fact that Laponite and biological molecules bands can be clearly differentiated.We would like to thank IDIVAL for financial support, Projects N°NVAL16/17, INNVAL19/18 and NVAL18/07. CRL thanks the MINECO for the Juan de la Cierva Formación grant (ref. FJCI-2015-25306). This work has been supported by the Spanish MINECO, Instituto de Salud Carlos III, the European Union FEDER funds under Projects ref. PI16/00496 (AES 2016), PI19/00349 and DTS19/00033 (AES 2019). The authors are grateful to Dr F Madrazo and the Laser Microscopy Unit of the IDIVAL Institute for the use of the Confocal Raman Imaging Microscope.ElsevierUniversidad de Cantabria20212021-01-30journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articlehttp://hdl.handle.net/10902/19264Applied Surface Science, 2021, 537, 147870reponame:UCrea Repositorio Abierto de la Universidad de Cantabriainstname:Universidad de Cantabria (UC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:repositorio.unican.es:10902/192642026-06-02T12:39:31Z |
| dc.title.none.fl_str_mv |
Free-labeled nanoclay intracellular uptake tracking by confocal Raman imaging |
| title |
Free-labeled nanoclay intracellular uptake tracking by confocal Raman imaging |
| spellingShingle |
Free-labeled nanoclay intracellular uptake tracking by confocal Raman imaging Iturrioz Rodríguez, Nerea Laponite Nanomaterial Nanocarrier Raman imaging technique Nanoclay |
| title_short |
Free-labeled nanoclay intracellular uptake tracking by confocal Raman imaging |
| title_full |
Free-labeled nanoclay intracellular uptake tracking by confocal Raman imaging |
| title_fullStr |
Free-labeled nanoclay intracellular uptake tracking by confocal Raman imaging |
| title_full_unstemmed |
Free-labeled nanoclay intracellular uptake tracking by confocal Raman imaging |
| title_sort |
Free-labeled nanoclay intracellular uptake tracking by confocal Raman imaging |
| dc.creator.none.fl_str_mv |
Iturrioz Rodríguez, Nerea Martín Rodríguez, Rosa Renero Lecuna, Carlos Aguado Menéndez, Fernando|||0000-0003-2912-0228 González Legarreta, Lorena|||0000-0001-5193-7256 González Gómez, Jesús Antonio|||0000-0002-0381-6393 López Fanarraga, Mónica|||0000-0003-4754-311X Perdigón Aller, Ana Carmen|||0000-0003-3865-3405 |
| author |
Iturrioz Rodríguez, Nerea |
| author_facet |
Iturrioz Rodríguez, Nerea Martín Rodríguez, Rosa Renero Lecuna, Carlos Aguado Menéndez, Fernando|||0000-0003-2912-0228 González Legarreta, Lorena|||0000-0001-5193-7256 González Gómez, Jesús Antonio|||0000-0002-0381-6393 López Fanarraga, Mónica|||0000-0003-4754-311X Perdigón Aller, Ana Carmen|||0000-0003-3865-3405 |
| author_role |
author |
| author2 |
Martín Rodríguez, Rosa Renero Lecuna, Carlos Aguado Menéndez, Fernando|||0000-0003-2912-0228 González Legarreta, Lorena|||0000-0001-5193-7256 González Gómez, Jesús Antonio|||0000-0002-0381-6393 López Fanarraga, Mónica|||0000-0003-4754-311X Perdigón Aller, Ana Carmen|||0000-0003-3865-3405 |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidad de Cantabria |
| dc.subject.none.fl_str_mv |
Laponite Nanomaterial Nanocarrier Raman imaging technique Nanoclay |
| topic |
Laponite Nanomaterial Nanocarrier Raman imaging technique Nanoclay |
| description |
Laponite is a nanoplatform that has been successfully used as a new biomaterial for drug delivery, tissue engineering and bioimaging at the nanoscale. In general, a deep knowledge of the mechanism interaction of the nanomaterial with biological components in a physiological environment is highly desirable for properly characterizing its therapeutic efficacy and toxicology. Up to know, the use of fluorescent dyes labelling both, the nanomaterial and cell components, has been a requirement to characterize the cell uptake and to visualize the entrance of the nanomaterial into the cytosol and the cell nucleus. The used of fluorophores usually perturb the physiological medium and can interfere in the nanomaterial cell interaction. A new Raman imaging methodology to track the uptake and internalization of Laponite nanoparticles into J774 macrophages line cells is presented in this work. The combination of Raman spectroscopy and confocal microscopy provides direct information about the localization of the nanoparticle into the cell, through its unique vibrational fingerprint without labelling or adding dyes, and taking advantage of the fact that Laponite and biological molecules bands can be clearly differentiated. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 2021-01-30 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 NA http://purl.org/coar/version/c_be7fb7dd8ff6fe43 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10902/19264 |
| url |
http://hdl.handle.net/10902/19264 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
| dc.source.none.fl_str_mv |
Applied Surface Science, 2021, 537, 147870 reponame:UCrea Repositorio Abierto de la Universidad de Cantabria instname:Universidad de Cantabria (UC) |
| instname_str |
Universidad de Cantabria (UC) |
| reponame_str |
UCrea Repositorio Abierto de la Universidad de Cantabria |
| collection |
UCrea Repositorio Abierto de la Universidad de Cantabria |
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1869406666984783872 |
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15,300719 |