Immunization with LytB protein of Streptococcus pneumoniae activates complement-mediated phagocytosis and induces protection against pneumonia and sepsis

34 p.-8 fig.

Detalles Bibliográficos
Autores: Corsini, B., Aguinagalde, Leire, Ruiz, Susana, Domenech, Mirian, Antequera, Marisa, Fenoll, Asunción, García González, Pedro, García, Ernesto, Yuste, José
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/143102
Acceso en línea:http://hdl.handle.net/10261/143102
Access Level:acceso abierto
Palabra clave:Streptococcus pneumoniae,
Cell wall hydrolases
LytB
Vaccine protein
Phagocytosis
Complement immunity
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spelling Immunization with LytB protein of Streptococcus pneumoniae activates complement-mediated phagocytosis and induces protection against pneumonia and sepsisCorsini, B.Aguinagalde, LeireRuiz, SusanaDomenech, MirianAntequera, MarisaFenoll, AsunciónGarcía González, PedroGarcía, ErnestoYuste, JoséStreptococcus pneumoniae,Cell wall hydrolasesLytBVaccine proteinPhagocytosisComplement immunity34 p.-8 fig.The cell wall glucosaminidase LytB of Streptococcus pneumoniae is a surface exposed protein involved in daughter cell separation, biofilm formation and contributes to different aspects of the pathogenesis process. In this study we have characterized the antibody responses after immunization of mice with LytB in the presence of alhydrogel as an adjuvant. Enzyme-linked immunosorbent assays measuring different subclasses of immunoglobulin G, demonstrated that the antibody responses to LytB were predominantly IgG1 and IgG2b, followed by IgG3 and IgG2a subclasses. Complement-mediated immunity against two different pneumococcal serotypes was investigated using sera from immunized mice. Immunization with LytB increased the recognition of S. pneumoniae by complement components C1q and C3b demonstrating that anti-LytB antibodies trigger activation of the classical pathway. Phagocytosis assays showed that serum containing antibodies to LytB stimulates neutrophil-mediated phagocytosis against S. pneumoniae. Animal models of infection including invasive pneumonia and sepsis were performed with two different clinical isolates. Vaccination with LytB increased bacterial clearance and induced protection demonstrating that LytB might be a good candidate to be considered in a future protein-based vaccine against S. pneumoniae.This work was supported by grants SAF2012-39444-C01/02 from Ministerio de Economía y Competitividad (MINECO). Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES) is an initiative of ISCIII. BC and LA were supported, respectively, by a fellowship from the Brazilian Program Ciencia Sem Fronteiras (CsF) from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and by an FPI fellowship from MINECOPeer reviewedElsevierMinisterio de Economía y Competitividad (España)Centro de Investigación Biomédica en Red Enfermedades Respiratorias (España)Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201720172016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/143102reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.vaccine.2016.11.001Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1431022026-05-22T06:33:51Z
dc.title.none.fl_str_mv Immunization with LytB protein of Streptococcus pneumoniae activates complement-mediated phagocytosis and induces protection against pneumonia and sepsis
title Immunization with LytB protein of Streptococcus pneumoniae activates complement-mediated phagocytosis and induces protection against pneumonia and sepsis
spellingShingle Immunization with LytB protein of Streptococcus pneumoniae activates complement-mediated phagocytosis and induces protection against pneumonia and sepsis
Corsini, B.
Streptococcus pneumoniae,
Cell wall hydrolases
LytB
Vaccine protein
Phagocytosis
Complement immunity
title_short Immunization with LytB protein of Streptococcus pneumoniae activates complement-mediated phagocytosis and induces protection against pneumonia and sepsis
title_full Immunization with LytB protein of Streptococcus pneumoniae activates complement-mediated phagocytosis and induces protection against pneumonia and sepsis
title_fullStr Immunization with LytB protein of Streptococcus pneumoniae activates complement-mediated phagocytosis and induces protection against pneumonia and sepsis
title_full_unstemmed Immunization with LytB protein of Streptococcus pneumoniae activates complement-mediated phagocytosis and induces protection against pneumonia and sepsis
title_sort Immunization with LytB protein of Streptococcus pneumoniae activates complement-mediated phagocytosis and induces protection against pneumonia and sepsis
dc.creator.none.fl_str_mv Corsini, B.
Aguinagalde, Leire
Ruiz, Susana
Domenech, Mirian
Antequera, Marisa
Fenoll, Asunción
García González, Pedro
García, Ernesto
Yuste, José
author Corsini, B.
author_facet Corsini, B.
Aguinagalde, Leire
Ruiz, Susana
Domenech, Mirian
Antequera, Marisa
Fenoll, Asunción
García González, Pedro
García, Ernesto
Yuste, José
author_role author
author2 Aguinagalde, Leire
Ruiz, Susana
Domenech, Mirian
Antequera, Marisa
Fenoll, Asunción
García González, Pedro
García, Ernesto
Yuste, José
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Centro de Investigación Biomédica en Red Enfermedades Respiratorias (España)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Streptococcus pneumoniae,
Cell wall hydrolases
LytB
Vaccine protein
Phagocytosis
Complement immunity
topic Streptococcus pneumoniae,
Cell wall hydrolases
LytB
Vaccine protein
Phagocytosis
Complement immunity
description 34 p.-8 fig.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/143102
url http://hdl.handle.net/10261/143102
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.vaccine.2016.11.001
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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