Saccharification of microalgae biomass obtained from wastewater treatment by enzymatic hydrolysis. Effect of alkaline-peroxide pretreatment

An enzymatic method for the carbohydrate hydrolysis of different microalgae biomass cultivated in domestic (DWB) and pig manure (PMWB) wastewaters, at different storage conditions (fresh, freeze-dried and reconstituted), was evaluated. The DWB provided sugars yields between 40 and 63%, although low...

ver descrição completa

Detalhes bibliográficos
Autores: Martín Juarez, Judit, Lorenzo Hernando, Ana María, Blanco, Saúl, Muñoz Torre, Raúl, Bolado Rodríguez, Silvia
Formato: artículo
Fecha de publicación:2016
País:España
Recursos:Universidad de Valladolid
Repositorio:UVaDOC. Repositorio Documental de la Universidad de Valladolid
OAI Identifier:oai:uvadoc.uva.es:10324/27891
Acesso em linha:http://uvadoc.uva.es/handle/10324/27891
Access Level:acceso abierto
Palavra-chave:Enzymatic hydrolysis
Glucose
Xylose
Wastewater
Alkaline-peroxide pretreatment
Descrição
Resumo:An enzymatic method for the carbohydrate hydrolysis of different microalgae biomass cultivated in domestic (DWB) and pig manure (PMWB) wastewaters, at different storage conditions (fresh, freeze-dried and reconstituted), was evaluated. The DWB provided sugars yields between 40 and 63%, although low xylose yields (< 23.5%). Approximately 2% of this biomass was converted to byproducts as succinic, acetic and formic acids. For PMWB, a high fraction of the sugars (up to 87%) was extracted, but mainly converted into acetic, butyric and formic acids, which was attributed to the bacterial action. In addition, the performance of an alkaline-peroxide pretreatment, conducted for 1 hour, 50ºC and H2O2 concentrations from 1 to 7.5% (w/w), was essayed. The hydrolysis of pretreated microalgae supported a wide range of sugars extraction for DWB (55-90%), and 100% for PMWB. Nevertheless, a large fraction of these sugars (~30% for DWB and 100% for PMWB) was transformed to byproducts.