ATP crossing the cell plasma membrane generates an ionic current in Xenopus oocytes

The presence of ATP within cells is well established. However, ATP also operates as an intercellular signal via specific purinoceptors. Furthermore, nonsecretory cells can release ATP under certain experimental conditions. To measure ATP release and membrane currents from a single cell simultaneousl...

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Detalles Bibliográficos
Autores: Bodas, Elena, Aleu, Jordi, Pujol, Gemma, Martín Satué, Mireia, Marsal Tebé, Jordi, Solsona Sancho, Carles
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2000
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/177100
Acceso en línea:https://hdl.handle.net/2445/177100
Access Level:acceso abierto
Palabra clave:Trifosfat d'adenosina
Membranes cel·lulars
Canals iònics
Adenosine triphospahatase
Cell membranes
Ion channels
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spelling ATP crossing the cell plasma membrane generates an ionic current in Xenopus oocytesBodas, ElenaAleu, JordiPujol, GemmaMartín Satué, MireiaMarsal Tebé, JordiSolsona Sancho, CarlesTrifosfat d'adenosinaMembranes cel·lularsCanals iònicsAdenosine triphospahataseCell membranesIon channelsThe presence of ATP within cells is well established. However, ATP also operates as an intercellular signal via specific purinoceptors. Furthermore, nonsecretory cells can release ATP under certain experimental conditions. To measure ATP release and membrane currents from a single cell simultaneously, we used Xenopus oocytes. We simultaneously recorded membrane currents and luminescence. Here, we show that ATP release can be triggered in Xenopus oocytes by hyperpolarizing pulses. ATP release (3.2 +/- 0.3 pmol/oocyte) generated a slow inward current (2.3 +/- 0.1 microA). During hyperpolarizing pulses, the permeability for ATP(4-) was more than 4000 times higher than that for Cl(-). The sensitivity to GdCl(3) (0. 2 mm) of hyperpolarization-induced ionic current, ATP release and E-ATPase activity suggests their dependence on stretch-activated ion channels. The pharmacological profile of the current inhibition coincides with the inhibition of ecto-ATPase activity. This enzyme is highly conserved among species, and in humans, it has been cloned and characterized as CD39. The translation, in Xenopus oocytes, of human CD39 mRNA encoding enhances the ATP-supported current, indicating that CD39 is directly or indirectly responsible for the electrodiffusion of ATP.American Society for Biochemistry and Molecular Biology2000info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/177100Articles publicats en revistes (Patologia i Terapèutica Experimental)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1074/jbc.M000894200Journal of Biological Chemistry, 2000, vol. 275, num. 27, p. 20268-20273https://doi.org/10.1074/jbc.M000894200(c) American Society for Biochemistry and Molecular Biology, 2000info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1771002026-05-27T06:46:51Z
dc.title.none.fl_str_mv ATP crossing the cell plasma membrane generates an ionic current in Xenopus oocytes
title ATP crossing the cell plasma membrane generates an ionic current in Xenopus oocytes
spellingShingle ATP crossing the cell plasma membrane generates an ionic current in Xenopus oocytes
Bodas, Elena
Trifosfat d'adenosina
Membranes cel·lulars
Canals iònics
Adenosine triphospahatase
Cell membranes
Ion channels
title_short ATP crossing the cell plasma membrane generates an ionic current in Xenopus oocytes
title_full ATP crossing the cell plasma membrane generates an ionic current in Xenopus oocytes
title_fullStr ATP crossing the cell plasma membrane generates an ionic current in Xenopus oocytes
title_full_unstemmed ATP crossing the cell plasma membrane generates an ionic current in Xenopus oocytes
title_sort ATP crossing the cell plasma membrane generates an ionic current in Xenopus oocytes
dc.creator.none.fl_str_mv Bodas, Elena
Aleu, Jordi
Pujol, Gemma
Martín Satué, Mireia
Marsal Tebé, Jordi
Solsona Sancho, Carles
author Bodas, Elena
author_facet Bodas, Elena
Aleu, Jordi
Pujol, Gemma
Martín Satué, Mireia
Marsal Tebé, Jordi
Solsona Sancho, Carles
author_role author
author2 Aleu, Jordi
Pujol, Gemma
Martín Satué, Mireia
Marsal Tebé, Jordi
Solsona Sancho, Carles
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Trifosfat d'adenosina
Membranes cel·lulars
Canals iònics
Adenosine triphospahatase
Cell membranes
Ion channels
topic Trifosfat d'adenosina
Membranes cel·lulars
Canals iònics
Adenosine triphospahatase
Cell membranes
Ion channels
description The presence of ATP within cells is well established. However, ATP also operates as an intercellular signal via specific purinoceptors. Furthermore, nonsecretory cells can release ATP under certain experimental conditions. To measure ATP release and membrane currents from a single cell simultaneously, we used Xenopus oocytes. We simultaneously recorded membrane currents and luminescence. Here, we show that ATP release can be triggered in Xenopus oocytes by hyperpolarizing pulses. ATP release (3.2 +/- 0.3 pmol/oocyte) generated a slow inward current (2.3 +/- 0.1 microA). During hyperpolarizing pulses, the permeability for ATP(4-) was more than 4000 times higher than that for Cl(-). The sensitivity to GdCl(3) (0. 2 mm) of hyperpolarization-induced ionic current, ATP release and E-ATPase activity suggests their dependence on stretch-activated ion channels. The pharmacological profile of the current inhibition coincides with the inhibition of ecto-ATPase activity. This enzyme is highly conserved among species, and in humans, it has been cloned and characterized as CD39. The translation, in Xenopus oocytes, of human CD39 mRNA encoding enhances the ATP-supported current, indicating that CD39 is directly or indirectly responsible for the electrodiffusion of ATP.
publishDate 2000
dc.date.none.fl_str_mv 2000
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/177100
url https://hdl.handle.net/2445/177100
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1074/jbc.M000894200
Journal of Biological Chemistry, 2000, vol. 275, num. 27, p. 20268-20273
https://doi.org/10.1074/jbc.M000894200
dc.rights.none.fl_str_mv (c) American Society for Biochemistry and Molecular Biology, 2000
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) American Society for Biochemistry and Molecular Biology, 2000
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv Articles publicats en revistes (Patologia i Terapèutica Experimental)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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