Biofunctional silk sericin hydrogels: A versatile platform with potential for tissue healing and regeneration

Discarded silk sericin protein (SS) presents a high yet underexplored potential as a biomaterial for tissue engineering (TE). Despite its biocompatibility, antioxidant activity, and moisture retention properties, its poor stability in aqueous media has limited broader application. In this work, we d...

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Authors: Veiga, Anabela, Ribeiro, Viviana, Ramírez-Jiménez, Rosa Ana, Aguilar de Armas, María Rosa, Rojo, Luis, Oliveira, Ana L.
Format: article
Status:Published version
Publication Date:2025
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/395840
Online Access:http://hdl.handle.net/10261/395840
https://api.elsevier.com/content/abstract/scopus_id/105010466786
Access Level:Open access
Keyword:Horseradish peroxidase (HRP)
Hydrogels
Silk sericin (SS)
Skin tissue engineering (TE)
Tannic acid (TA)
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oai_identifier_str oai:digital.csic.es:10261/395840
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Biofunctional silk sericin hydrogels: A versatile platform with potential for tissue healing and regeneration
title Biofunctional silk sericin hydrogels: A versatile platform with potential for tissue healing and regeneration
spellingShingle Biofunctional silk sericin hydrogels: A versatile platform with potential for tissue healing and regeneration
Veiga, Anabela
Horseradish peroxidase (HRP)
Hydrogels
Silk sericin (SS)
Skin tissue engineering (TE)
Tannic acid (TA)
title_short Biofunctional silk sericin hydrogels: A versatile platform with potential for tissue healing and regeneration
title_full Biofunctional silk sericin hydrogels: A versatile platform with potential for tissue healing and regeneration
title_fullStr Biofunctional silk sericin hydrogels: A versatile platform with potential for tissue healing and regeneration
title_full_unstemmed Biofunctional silk sericin hydrogels: A versatile platform with potential for tissue healing and regeneration
title_sort Biofunctional silk sericin hydrogels: A versatile platform with potential for tissue healing and regeneration
dc.creator.none.fl_str_mv Veiga, Anabela
Ribeiro, Viviana
Ramírez-Jiménez, Rosa Ana
Aguilar de Armas, María Rosa
Rojo, Luis
Oliveira, Ana L.
author Veiga, Anabela
author_facet Veiga, Anabela
Ribeiro, Viviana
Ramírez-Jiménez, Rosa Ana
Aguilar de Armas, María Rosa
Rojo, Luis
Oliveira, Ana L.
author_role author
author2 Ribeiro, Viviana
Ramírez-Jiménez, Rosa Ana
Aguilar de Armas, María Rosa
Rojo, Luis
Oliveira, Ana L.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministério da Ciência, Tecnologia e Ensino Superior (Portugal)
Ministerio de Ciencia e Innovación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
European Commission
Comunidad de Madrid
Veiga, Anabela [0000-0002-5677-9629]
Ribeiro, Viviana [0000-0002-3679-0759]
Ramírez-Jiménez, Rosa Ana [0000-0001-7563-2553]
Aguilar, Maria Rosa [0000-0001-7395-5754]
Rojo, Luis [0000-0001-6334-6736]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Horseradish peroxidase (HRP)
Hydrogels
Silk sericin (SS)
Skin tissue engineering (TE)
Tannic acid (TA)
topic Horseradish peroxidase (HRP)
Hydrogels
Silk sericin (SS)
Skin tissue engineering (TE)
Tannic acid (TA)
description Discarded silk sericin protein (SS) presents a high yet underexplored potential as a biomaterial for tissue engineering (TE). Despite its biocompatibility, antioxidant activity, and moisture retention properties, its poor stability in aqueous media has limited broader application. In this work, we developed and characterized SS-based hydrogels using tannic acid (TA) and horseradish peroxidase (HRP) crosslinking systems to address these limitations and expand their use in skin TE. Hydrogels were prepared using SS concentrations of 2.5 % and 5 % (w/v) and evaluated for rheological behavior (G' ranging from 100 to 10,000 Pa), swelling (up to 24 %), retention capacity (stable over 24-30 h), and degradation in proteolytic environments (mass loss ranging from ∼0-11 %, depending on formulation). TA-crosslinked hydrogels showed strong fluid retention and are suitable for high-moisture 3D wound dressings and coating applications. HRP-crosslinked hydrogels demonstrated tunable mechanical properties, shear-thinning behavior, and full recovery post-deformation, making them ideal for use as bioinks in 3D bioprinting and injectable matrices. In vitro assays confirmed cytocompatibility, with viability exceeding 85 %, and successful cell encapsulation and proliferation. Overall, this study presents a versatile SS-based hydrogel platform with potential for various biomedical applications, particularly in skin tissue healing and regeneration.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/395840
https://api.elsevier.com/content/abstract/scopus_id/105010466786
url http://hdl.handle.net/10261/395840
https://api.elsevier.com/content/abstract/scopus_id/105010466786
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-114086RB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-149301OB-I00
https://doi.org/10.1016/j.colsurfb.2025.114916
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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spelling Biofunctional silk sericin hydrogels: A versatile platform with potential for tissue healing and regenerationVeiga, AnabelaRibeiro, VivianaRamírez-Jiménez, Rosa AnaAguilar de Armas, María RosaRojo, LuisOliveira, Ana L.Horseradish peroxidase (HRP)HydrogelsSilk sericin (SS)Skin tissue engineering (TE)Tannic acid (TA)Discarded silk sericin protein (SS) presents a high yet underexplored potential as a biomaterial for tissue engineering (TE). Despite its biocompatibility, antioxidant activity, and moisture retention properties, its poor stability in aqueous media has limited broader application. In this work, we developed and characterized SS-based hydrogels using tannic acid (TA) and horseradish peroxidase (HRP) crosslinking systems to address these limitations and expand their use in skin TE. Hydrogels were prepared using SS concentrations of 2.5 % and 5 % (w/v) and evaluated for rheological behavior (G' ranging from 100 to 10,000 Pa), swelling (up to 24 %), retention capacity (stable over 24-30 h), and degradation in proteolytic environments (mass loss ranging from ∼0-11 %, depending on formulation). TA-crosslinked hydrogels showed strong fluid retention and are suitable for high-moisture 3D wound dressings and coating applications. HRP-crosslinked hydrogels demonstrated tunable mechanical properties, shear-thinning behavior, and full recovery post-deformation, making them ideal for use as bioinks in 3D bioprinting and injectable matrices. In vitro assays confirmed cytocompatibility, with viability exceeding 85 %, and successful cell encapsulation and proliferation. Overall, this study presents a versatile SS-based hydrogel platform with potential for various biomedical applications, particularly in skin tissue healing and regeneration.This work was financially supported by: National Funds through FCT (Foundation for Science and Technology) under the project UIDB/ 50016/2020 of the Centre for Biotechnology and Fine Chemistry - CBQF; by LA/P/0045/2020 (ALiCE), UIDB/00511/2020 and UIDP/00511/ 2020 (LEPABE), funded by national funds through FCT/MCTES (PID- DAC); and IBEROS+ (0072_IBEROS_MAIS_1_E) - Instituto de Bio- fabricación en Red para El Envejecimiento Saludable, funded by “Interreg VI A Españaa – Portugal (POCTEP) 2021–2027. A. Veiga gratefully acknowledges doctoral scholarship [2020.08683.BD] from FCT and ERASMUS + mobility scholarship from the Faculty of biotechnology-Portuguese catholic University (ESB-UCP). MRA and LR are members of Technological Interdisciplinary Platform SUS- PLAST+ and were supported by MICINN PID2020–114086RB-100, PID2023–149301OB-I00 funded by MICIU/AEI (DOI: 10.13039/ 501100011033) and by ERDF/EU and Autonomous Community of Madrid, Spain, grant S2022/BMD-7406 and by CIBER-BBN (Spain) – Consorcio Centro de Investigación Biomédica en Red (Ref. CB06/01/ 0013). VR acknowledge the Individual Junior Research contract 2023.07374.CEECIND/CP2855/CT0006 attributed by FCT.Peer reviewedElsevierMinistério da Ciência, Tecnologia e Ensino Superior (Portugal)Ministerio de Ciencia e Innovación (España)Ministerio de Ciencia, Innovación y Universidades (España)European CommissionComunidad de MadridVeiga, Anabela [0000-0002-5677-9629]Ribeiro, Viviana [0000-0002-3679-0759]Ramírez-Jiménez, Rosa Ana [0000-0001-7563-2553]Aguilar, Maria Rosa [0000-0001-7395-5754]Rojo, Luis [0000-0001-6334-6736]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/395840https://api.elsevier.com/content/abstract/scopus_id/105010466786reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-114086RB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-149301OB-I00https://doi.org/10.1016/j.colsurfb.2025.114916Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3958402026-05-22T06:33:51Z
score 15,812455

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