CDK2 regulates nuclear envelope protein dynamics and telomere attachment in mouse meiotic prophase

In most organisms, telomeres attach to the nuclear envelope at the onset of meiosis to promote the crucial processes of pairing, recombination and synapsis during prophase I. This attachment of meiotic telomeres is mediated by the specific distribution of several nuclear envelope components that int...

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Detalhes bibliográficos
Autores: Viera Vicario, Alberto, Alsheimer, M., Gómez Lencero, Rocío, Berenguer, I., Ortega, S., Symonds, C.E., Santamaría, Daniel F., Benavente, R., Suja Sánchez, José Ángel
Formato: artículo
Fecha de publicación:2015
País:España
Recursos:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/676450
Acesso em linha:http://hdl.handle.net/10486/676450
https://dx.doi.org/10.1242/jcs.154922
Access Level:acceso abierto
Palavra-chave:CDK2
Meiosis
Nuclear envelope
SUN1
Telomere
Biología y Biomedicina / Biología
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spelling CDK2 regulates nuclear envelope protein dynamics and telomere attachment in mouse meiotic prophaseViera Vicario, AlbertoAlsheimer, M.Gómez Lencero, RocíoBerenguer, I.Ortega, S.Symonds, C.E.Santamaría, Daniel F.Benavente, R.Suja Sánchez, José ÁngelCDK2MeiosisNuclear envelopeSUN1TelomereBiología y Biomedicina / BiologíaIn most organisms, telomeres attach to the nuclear envelope at the onset of meiosis to promote the crucial processes of pairing, recombination and synapsis during prophase I. This attachment of meiotic telomeres is mediated by the specific distribution of several nuclear envelope components that interact with the attachment plates of the synaptonemal complex. We have determined by immunofluorescence and electron microscopy that the ablation of the kinase CDK2 alters the nuclear envelope in mouse spermatocytes, and that the proteins SUN1, KASH5 (also known as CCDC155) and lamin C2 show an abnormal cap-like distribution facing the centrosome. Strikingly, some telomeres are not attached to the nuclear envelope but remain at the nuclear interior where they are associated with SUN1 and with nuclear-envelope-detached vesicles. We also demonstrate that mouse testis CDK2 phosphorylates SUN1 in vitro. We propose that during mammalian prophase I the kinase CDK2 is a key factor governing the structure of the nuclear envelope and the telomere-led chromosome movements essential for homolog pairing© 2015. Published by The Company of Biologists Ltd.Departamento de BiologíaFacultad de Ciencias20152015-01-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/676450https://dx.doi.org/10.1242/jcs.154922reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6764502026-06-23T12:46:27Z
dc.title.none.fl_str_mv CDK2 regulates nuclear envelope protein dynamics and telomere attachment in mouse meiotic prophase
title CDK2 regulates nuclear envelope protein dynamics and telomere attachment in mouse meiotic prophase
spellingShingle CDK2 regulates nuclear envelope protein dynamics and telomere attachment in mouse meiotic prophase
Viera Vicario, Alberto
CDK2
Meiosis
Nuclear envelope
SUN1
Telomere
Biología y Biomedicina / Biología
title_short CDK2 regulates nuclear envelope protein dynamics and telomere attachment in mouse meiotic prophase
title_full CDK2 regulates nuclear envelope protein dynamics and telomere attachment in mouse meiotic prophase
title_fullStr CDK2 regulates nuclear envelope protein dynamics and telomere attachment in mouse meiotic prophase
title_full_unstemmed CDK2 regulates nuclear envelope protein dynamics and telomere attachment in mouse meiotic prophase
title_sort CDK2 regulates nuclear envelope protein dynamics and telomere attachment in mouse meiotic prophase
dc.creator.none.fl_str_mv Viera Vicario, Alberto
Alsheimer, M.
Gómez Lencero, Rocío
Berenguer, I.
Ortega, S.
Symonds, C.E.
Santamaría, Daniel F.
Benavente, R.
Suja Sánchez, José Ángel
author Viera Vicario, Alberto
author_facet Viera Vicario, Alberto
Alsheimer, M.
Gómez Lencero, Rocío
Berenguer, I.
Ortega, S.
Symonds, C.E.
Santamaría, Daniel F.
Benavente, R.
Suja Sánchez, José Ángel
author_role author
author2 Alsheimer, M.
Gómez Lencero, Rocío
Berenguer, I.
Ortega, S.
Symonds, C.E.
Santamaría, Daniel F.
Benavente, R.
Suja Sánchez, José Ángel
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Biología
Facultad de Ciencias
dc.subject.none.fl_str_mv CDK2
Meiosis
Nuclear envelope
SUN1
Telomere
Biología y Biomedicina / Biología
topic CDK2
Meiosis
Nuclear envelope
SUN1
Telomere
Biología y Biomedicina / Biología
description In most organisms, telomeres attach to the nuclear envelope at the onset of meiosis to promote the crucial processes of pairing, recombination and synapsis during prophase I. This attachment of meiotic telomeres is mediated by the specific distribution of several nuclear envelope components that interact with the attachment plates of the synaptonemal complex. We have determined by immunofluorescence and electron microscopy that the ablation of the kinase CDK2 alters the nuclear envelope in mouse spermatocytes, and that the proteins SUN1, KASH5 (also known as CCDC155) and lamin C2 show an abnormal cap-like distribution facing the centrosome. Strikingly, some telomeres are not attached to the nuclear envelope but remain at the nuclear interior where they are associated with SUN1 and with nuclear-envelope-detached vesicles. We also demonstrate that mouse testis CDK2 phosphorylates SUN1 in vitro. We propose that during mammalian prophase I the kinase CDK2 is a key factor governing the structure of the nuclear envelope and the telomere-led chromosome movements essential for homolog pairing
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/676450
https://dx.doi.org/10.1242/jcs.154922
url http://hdl.handle.net/10486/676450
https://dx.doi.org/10.1242/jcs.154922
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv © 2015. Published by The Company of Biologists Ltd.
publisher.none.fl_str_mv © 2015. Published by The Company of Biologists Ltd.
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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