Spread of ST348 Klebsiella pneumoniae producing NDM-1 in a peruvian hospital

The aim of this study was to characterize carbapenem-resistant Klebsiella pneumoniae (CR-Kp) isolates recovered from adults and children with severe bacteremia in a Peruvian Hospital in June 2018. Antimicrobial susceptibility was determined by disc/gradient diffusion and broth microdilution when nec...

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Autores: Pons, María J., Marí Almirall, Marta, Ymaña, Bárbara, Moya Salazar, Jeel, Muñoz López, Laura, Sauñe, Sharon, Salazar Hernández, Richard, Vila Estapé, Jordi, Roca Subirà, Ignasi
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/174155
Acceso en línea:https://hdl.handle.net/2445/174155
Access Level:acceso abierto
Palabra clave:Klebsiella pneumoniae
Bacteris
Perú
Bacteria
Peru
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spelling Spread of ST348 Klebsiella pneumoniae producing NDM-1 in a peruvian hospitalPons, María J.Marí Almirall, MartaYmaña, BárbaraMoya Salazar, JeelMuñoz López, LauraSauñe, SharonSalazar Hernández, RichardVila Estapé, JordiRoca Subirà, IgnasiKlebsiella pneumoniaeBacterisPerúKlebsiella pneumoniaeBacteriaPeruThe aim of this study was to characterize carbapenem-resistant Klebsiella pneumoniae (CR-Kp) isolates recovered from adults and children with severe bacteremia in a Peruvian Hospital in June 2018. Antimicrobial susceptibility was determined by disc/gradient diffusion and broth microdilution when necessary. Antibiotic resistance mechanisms were evaluated by PCR and DNA sequencing. Clonal relatedness was assessed using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Plasmid typing was performed with a PCR-based method. Thirty CR-Kp isolates were recovered in June 2018. All isolates were non-susceptible to all -lactams, ciprofloxacin, gentamicin and trimethoprim-sulfamethoxazole, while mostly remaining susceptible to colistin, tigecycline, levofloxacin and amikacin. All isolates carried the blaNDM-1 gene and were extended spectrum -lactamase (ESBL) producers. PFGE showed four different pulsotypes although all isolates but two belonged to the ST348 sequence type, previously reported in Portugal. blaNDM-1 was located in an IncFIB-M conjugative plasmid. To our knowledge, this is the first report of an New Delhi metallo- -lactamase (NDM)-producing K. pneumoniae recovered from both children and adults in Lima, Peru, as well as the first time that the outbreak strain ST348 is reported in Peru and is associated with NDM. Studies providing epidemiological and molecular data on CR-Kp in Peru are essential to monitor their dissemination and prevent further spread.MDPI2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/174155Articles publicats en revistes (Fonaments Clínics)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/microorganisms8091392Microorganisms, 2020, vol. 8(9), num. 1392https://doi.org/10.3390/microorganisms8091392cc-by (c) Pons, María J. et al., 2020http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1741552026-05-27T06:46:51Z
dc.title.none.fl_str_mv Spread of ST348 Klebsiella pneumoniae producing NDM-1 in a peruvian hospital
title Spread of ST348 Klebsiella pneumoniae producing NDM-1 in a peruvian hospital
spellingShingle Spread of ST348 Klebsiella pneumoniae producing NDM-1 in a peruvian hospital
Pons, María J.
Klebsiella pneumoniae
Bacteris
Perú
Klebsiella pneumoniae
Bacteria
Peru
title_short Spread of ST348 Klebsiella pneumoniae producing NDM-1 in a peruvian hospital
title_full Spread of ST348 Klebsiella pneumoniae producing NDM-1 in a peruvian hospital
title_fullStr Spread of ST348 Klebsiella pneumoniae producing NDM-1 in a peruvian hospital
title_full_unstemmed Spread of ST348 Klebsiella pneumoniae producing NDM-1 in a peruvian hospital
title_sort Spread of ST348 Klebsiella pneumoniae producing NDM-1 in a peruvian hospital
dc.creator.none.fl_str_mv Pons, María J.
Marí Almirall, Marta
Ymaña, Bárbara
Moya Salazar, Jeel
Muñoz López, Laura
Sauñe, Sharon
Salazar Hernández, Richard
Vila Estapé, Jordi
Roca Subirà, Ignasi
author Pons, María J.
author_facet Pons, María J.
Marí Almirall, Marta
Ymaña, Bárbara
Moya Salazar, Jeel
Muñoz López, Laura
Sauñe, Sharon
Salazar Hernández, Richard
Vila Estapé, Jordi
Roca Subirà, Ignasi
author_role author
author2 Marí Almirall, Marta
Ymaña, Bárbara
Moya Salazar, Jeel
Muñoz López, Laura
Sauñe, Sharon
Salazar Hernández, Richard
Vila Estapé, Jordi
Roca Subirà, Ignasi
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Klebsiella pneumoniae
Bacteris
Perú
Klebsiella pneumoniae
Bacteria
Peru
topic Klebsiella pneumoniae
Bacteris
Perú
Klebsiella pneumoniae
Bacteria
Peru
description The aim of this study was to characterize carbapenem-resistant Klebsiella pneumoniae (CR-Kp) isolates recovered from adults and children with severe bacteremia in a Peruvian Hospital in June 2018. Antimicrobial susceptibility was determined by disc/gradient diffusion and broth microdilution when necessary. Antibiotic resistance mechanisms were evaluated by PCR and DNA sequencing. Clonal relatedness was assessed using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Plasmid typing was performed with a PCR-based method. Thirty CR-Kp isolates were recovered in June 2018. All isolates were non-susceptible to all -lactams, ciprofloxacin, gentamicin and trimethoprim-sulfamethoxazole, while mostly remaining susceptible to colistin, tigecycline, levofloxacin and amikacin. All isolates carried the blaNDM-1 gene and were extended spectrum -lactamase (ESBL) producers. PFGE showed four different pulsotypes although all isolates but two belonged to the ST348 sequence type, previously reported in Portugal. blaNDM-1 was located in an IncFIB-M conjugative plasmid. To our knowledge, this is the first report of an New Delhi metallo- -lactamase (NDM)-producing K. pneumoniae recovered from both children and adults in Lima, Peru, as well as the first time that the outbreak strain ST348 is reported in Peru and is associated with NDM. Studies providing epidemiological and molecular data on CR-Kp in Peru are essential to monitor their dissemination and prevent further spread.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/174155
url https://hdl.handle.net/2445/174155
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/microorganisms8091392
Microorganisms, 2020, vol. 8(9), num. 1392
https://doi.org/10.3390/microorganisms8091392
dc.rights.none.fl_str_mv cc-by (c) Pons, María J. et al., 2020
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Pons, María J. et al., 2020
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Fonaments Clínics)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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