Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System

Lipoic acid (LA, 6,8-dithiooctanoic acid) is a sulfur containing coenzyme essential for the activity of several key enzymes involved in oxidative and single carbon metabolism in most bacteria and eukaryotes. LA is synthetized by the concerted activity of the octanoyltransferase (LIP2, EC 2.3.1.181)...

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Autores: Martins Noguerol, Raquel, Acket, Sébastien, Troncoso Ponce, Manuel Adrián, Garcés Mancheño, Rafael, Thomasset, Brigitte, Venegas Calerón, Mónica, Salas, Joaquín J., Martínez Force, Enrique, Moreno Pérez, Antonio Javier
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2021
País:España
Recursos:Universidad de Sevilla (US)
Repositório:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/130538
Acesso em linha:https://hdl.handle.net/11441/130538
https://doi.org/10.3389/fpls.2021.781917
Access Level:Acceso aberto
Palavra-chave:Sunflower
Octanoyltransferase
Lipoyl synthase
Lipoic acid
Mitochondrial lipoylation
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spelling Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme SystemMartins Noguerol, RaquelAcket, SébastienTroncoso Ponce, Manuel AdriánGarcés Mancheño, RafaelThomasset, BrigitteVenegas Calerón, MónicaSalas, Joaquín J.Martínez Force, EnriqueMoreno Pérez, Antonio JavierSunflowerOctanoyltransferaseLipoyl synthaseLipoic acidMitochondrial lipoylationLipoic acid (LA, 6,8-dithiooctanoic acid) is a sulfur containing coenzyme essential for the activity of several key enzymes involved in oxidative and single carbon metabolism in most bacteria and eukaryotes. LA is synthetized by the concerted activity of the octanoyltransferase (LIP2, EC 2.3.1.181) and lipoyl synthase (LIP1, EC 2.8.1.8) enzymes. In plants, pyruvate dehydrogenase (PDH), 2-oxoglutarate dehydrogenase or glycine decarboxylase are essential complexes that need to be lipoylated. These lipoylated enzymes and complexes are located in the mitochondria, while PDH is also present in plastids where it provides acetyl-CoA for de novo fatty acid biosynthesis. As such, lipoylation of PDH could regulate fatty acid synthesis in both these organelles. In the present work, the sunflower LIP1 and LIP2 genes (HaLIP1m and HaLIP2m) were isolated sequenced, cloned, and characterized, evaluating their putative mitochondrial location. The expression of these genes was studied in different tissues and protein docking was modeled. The genes were also expressed in Escherichia coli and Arabidopsis thaliana, where their impact on fatty acid and glycerolipid composition was assessed. Lipidomic studies in Arabidopsis revealed lipid remodeling in lines overexpressing these enzymes and the involvement of both sunflower proteins in the phenotypes observed is discussed in the light of the results obtained.Frontiers MediaBiología Vegetal y Ecología2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/130538https://doi.org/10.3389/fpls.2021.781917reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésFrontiers in Plant Science, 12, 781917.https://doi.org/10.3389/fpls.2021.781917info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1305382026-06-17T12:51:07Z
dc.title.none.fl_str_mv Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System
title Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System
spellingShingle Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System
Martins Noguerol, Raquel
Sunflower
Octanoyltransferase
Lipoyl synthase
Lipoic acid
Mitochondrial lipoylation
title_short Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System
title_full Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System
title_fullStr Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System
title_full_unstemmed Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System
title_sort Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System
dc.creator.none.fl_str_mv Martins Noguerol, Raquel
Acket, Sébastien
Troncoso Ponce, Manuel Adrián
Garcés Mancheño, Rafael
Thomasset, Brigitte
Venegas Calerón, Mónica
Salas, Joaquín J.
Martínez Force, Enrique
Moreno Pérez, Antonio Javier
author Martins Noguerol, Raquel
author_facet Martins Noguerol, Raquel
Acket, Sébastien
Troncoso Ponce, Manuel Adrián
Garcés Mancheño, Rafael
Thomasset, Brigitte
Venegas Calerón, Mónica
Salas, Joaquín J.
Martínez Force, Enrique
Moreno Pérez, Antonio Javier
author_role author
author2 Acket, Sébastien
Troncoso Ponce, Manuel Adrián
Garcés Mancheño, Rafael
Thomasset, Brigitte
Venegas Calerón, Mónica
Salas, Joaquín J.
Martínez Force, Enrique
Moreno Pérez, Antonio Javier
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Biología Vegetal y Ecología
dc.subject.none.fl_str_mv Sunflower
Octanoyltransferase
Lipoyl synthase
Lipoic acid
Mitochondrial lipoylation
topic Sunflower
Octanoyltransferase
Lipoyl synthase
Lipoic acid
Mitochondrial lipoylation
description Lipoic acid (LA, 6,8-dithiooctanoic acid) is a sulfur containing coenzyme essential for the activity of several key enzymes involved in oxidative and single carbon metabolism in most bacteria and eukaryotes. LA is synthetized by the concerted activity of the octanoyltransferase (LIP2, EC 2.3.1.181) and lipoyl synthase (LIP1, EC 2.8.1.8) enzymes. In plants, pyruvate dehydrogenase (PDH), 2-oxoglutarate dehydrogenase or glycine decarboxylase are essential complexes that need to be lipoylated. These lipoylated enzymes and complexes are located in the mitochondria, while PDH is also present in plastids where it provides acetyl-CoA for de novo fatty acid biosynthesis. As such, lipoylation of PDH could regulate fatty acid synthesis in both these organelles. In the present work, the sunflower LIP1 and LIP2 genes (HaLIP1m and HaLIP2m) were isolated sequenced, cloned, and characterized, evaluating their putative mitochondrial location. The expression of these genes was studied in different tissues and protein docking was modeled. The genes were also expressed in Escherichia coli and Arabidopsis thaliana, where their impact on fatty acid and glycerolipid composition was assessed. Lipidomic studies in Arabidopsis revealed lipid remodeling in lines overexpressing these enzymes and the involvement of both sunflower proteins in the phenotypes observed is discussed in the light of the results obtained.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/130538
https://doi.org/10.3389/fpls.2021.781917
url https://hdl.handle.net/11441/130538
https://doi.org/10.3389/fpls.2021.781917
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Frontiers in Plant Science, 12, 781917.
https://doi.org/10.3389/fpls.2021.781917
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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