Muscle transcriptomic profiles in pigs with divergent phenotypes for fatness traits

BACKGROUND: Selection for increasing intramuscular fat content would definitively improve the palatability and juiciness of pig meat as well as the sensorial and organoleptic properties of cured products. However, evidences obtained in human and model organisms suggest that high levels of intramuscu...

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Autores: Cánovas Tienda, Angela, Quintanilla Aguado, Raquel, Amills i Eras, Marcel, Pena i Subirà, Ramona Natacha
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:España
Recursos:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/30332
Acesso em linha:https://doi.org/10.1186/1471-2164-11-372
http://hdl.handle.net/10459.1/30332
Access Level:acceso abierto
Palavra-chave:Porcs -- Genètica
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spelling Muscle transcriptomic profiles in pigs with divergent phenotypes for fatness traitsCánovas Tienda, AngelaQuintanilla Aguado, RaquelAmills i Eras, MarcelPena i Subirà, Ramona NatachaPorcs -- GenèticaBACKGROUND: Selection for increasing intramuscular fat content would definitively improve the palatability and juiciness of pig meat as well as the sensorial and organoleptic properties of cured products. However, evidences obtained in human and model organisms suggest that high levels of intramuscular fat might alter muscle lipid and carbohydrate metabolism. We have analysed this issue by determining the transcriptomic profiles of Duroc pigs with divergent phenotypes for 13 fatness traits. The strong aptitude of Duroc pigs to have high levels of intramuscular fat makes them a valuable model to analyse the mechanisms that regulate muscle lipid metabolism, an issue with evident implications in the elucidation of the genetic basis of human metabolic diseases such as obesity and insulin resistance. RESULTS: Muscle gene expression profiles of 68 Duroc pigs belonging to two groups (HIGH and LOW) with extreme phenotypes for lipid deposition and composition traits have been analysed. Microarray and quantitative PCR analysis showed that genes related to fatty acid uptake, lipogenesis and triacylglycerol synthesis were upregulated in the muscle tissue of HIGH pigs, which are fatter and have higher amounts of intramuscular fat than their LOW counterparts. Paradoxically, lipolytic genes also showed increased mRNA levels in the HIGH group suggesting the existence of a cycle where triacylglycerols are continuously synthesized and degraded. Several genes related to the insulin-signalling pathway, that is usually impaired in obese humans, were also upregulated. Finally, genes related to antigen-processing and presentation were downregulated in the HIGH group. CONCLUSION: Our data suggest that selection for increasing intramuscular fat content in pigs would lead to a shift but not a disruption of the metabolic homeostasis of muscle cells. Future studies on the post-translational changes affecting protein activity or expression as well as information about protein location within the cell would be needed to to elucidate the effects of lipid deposition on muscle metabolism in pigs.BioMed Central2010info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.1186/1471-2164-11-372http://hdl.handle.net/10459.1/30332reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)InglésReproducció del document publicat a https://doi.org/10.1186/1471-2164-11-372BMC Genomics, 2010, vol. 11, núm. 372, p. 1-15cc-by, (c) Cánovas et al., 2010info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.0/es/deed.caoai:repositori.udl.cat:10459.1/303322026-06-24T12:42:17Z
dc.title.none.fl_str_mv Muscle transcriptomic profiles in pigs with divergent phenotypes for fatness traits
title Muscle transcriptomic profiles in pigs with divergent phenotypes for fatness traits
spellingShingle Muscle transcriptomic profiles in pigs with divergent phenotypes for fatness traits
Cánovas Tienda, Angela
Porcs -- Genètica
title_short Muscle transcriptomic profiles in pigs with divergent phenotypes for fatness traits
title_full Muscle transcriptomic profiles in pigs with divergent phenotypes for fatness traits
title_fullStr Muscle transcriptomic profiles in pigs with divergent phenotypes for fatness traits
title_full_unstemmed Muscle transcriptomic profiles in pigs with divergent phenotypes for fatness traits
title_sort Muscle transcriptomic profiles in pigs with divergent phenotypes for fatness traits
dc.creator.none.fl_str_mv Cánovas Tienda, Angela
Quintanilla Aguado, Raquel
Amills i Eras, Marcel
Pena i Subirà, Ramona Natacha
author Cánovas Tienda, Angela
author_facet Cánovas Tienda, Angela
Quintanilla Aguado, Raquel
Amills i Eras, Marcel
Pena i Subirà, Ramona Natacha
author_role author
author2 Quintanilla Aguado, Raquel
Amills i Eras, Marcel
Pena i Subirà, Ramona Natacha
author2_role author
author
author
dc.subject.none.fl_str_mv Porcs -- Genètica
topic Porcs -- Genètica
description BACKGROUND: Selection for increasing intramuscular fat content would definitively improve the palatability and juiciness of pig meat as well as the sensorial and organoleptic properties of cured products. However, evidences obtained in human and model organisms suggest that high levels of intramuscular fat might alter muscle lipid and carbohydrate metabolism. We have analysed this issue by determining the transcriptomic profiles of Duroc pigs with divergent phenotypes for 13 fatness traits. The strong aptitude of Duroc pigs to have high levels of intramuscular fat makes them a valuable model to analyse the mechanisms that regulate muscle lipid metabolism, an issue with evident implications in the elucidation of the genetic basis of human metabolic diseases such as obesity and insulin resistance. RESULTS: Muscle gene expression profiles of 68 Duroc pigs belonging to two groups (HIGH and LOW) with extreme phenotypes for lipid deposition and composition traits have been analysed. Microarray and quantitative PCR analysis showed that genes related to fatty acid uptake, lipogenesis and triacylglycerol synthesis were upregulated in the muscle tissue of HIGH pigs, which are fatter and have higher amounts of intramuscular fat than their LOW counterparts. Paradoxically, lipolytic genes also showed increased mRNA levels in the HIGH group suggesting the existence of a cycle where triacylglycerols are continuously synthesized and degraded. Several genes related to the insulin-signalling pathway, that is usually impaired in obese humans, were also upregulated. Finally, genes related to antigen-processing and presentation were downregulated in the HIGH group. CONCLUSION: Our data suggest that selection for increasing intramuscular fat content in pigs would lead to a shift but not a disruption of the metabolic homeostasis of muscle cells. Future studies on the post-translational changes affecting protein activity or expression as well as information about protein location within the cell would be needed to to elucidate the effects of lipid deposition on muscle metabolism in pigs.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1186/1471-2164-11-372
http://hdl.handle.net/10459.1/30332
url https://doi.org/10.1186/1471-2164-11-372
http://hdl.handle.net/10459.1/30332
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a https://doi.org/10.1186/1471-2164-11-372
BMC Genomics, 2010, vol. 11, núm. 372, p. 1-15
dc.rights.none.fl_str_mv cc-by, (c) Cánovas et al., 2010
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.0/es/deed.ca
rights_invalid_str_mv cc-by, (c) Cánovas et al., 2010
http://creativecommons.org/licenses/by/2.0/es/deed.ca
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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