Unveiling the unusual i-motif-derived architecture of a DNA aptamer exhibiting high affinity for influenza A virus

17 pages, 7 figures, 3 tables

Detalles Bibliográficos
Autores: Tsvetkov, Vladimir, Mir, Bartomeu, Alieva, Rugiya, Arutyunyan, Alexander, Oleynikov, Ilya, Novikov, Roman, Boravleva, Elizaveta, Kamzeeva, Polina, Zatsepin, Timofei, Aralov, Andrey, González, Carlos, Zavyalova, Elena
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/380047
Acceso en línea:http://hdl.handle.net/10261/380047
https://api.elsevier.com/content/abstract/scopus_id/85214506988
Access Level:acceso abierto
Palabra clave:http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
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spelling Unveiling the unusual i-motif-derived architecture of a DNA aptamer exhibiting high affinity for influenza A virusTsvetkov, VladimirMir, BartomeuAlieva, RugiyaArutyunyan, AlexanderOleynikov, IlyaNovikov, RomanBoravleva, ElizavetaKamzeeva, PolinaZatsepin, TimofeiAralov, AndreyGonzález, CarlosZavyalova, Elenahttp://metadata.un.org/sdg/3Ensure healthy lives and promote well-being for all at all ages17 pages, 7 figures, 3 tablesNon-canonical nucleic acid structures play significant roles in cellular processes through selective interactions with proteins. While both natural and artificial G-quadruplexes have been extensively studied, the functions of i-motifs remain less understood. This study investigates the artificial aptamer BV42, which binds strongly to influenza A virus hemagglutinin and unexpectedly retains its i-motif structure even at neutral pH. However, BV42 conformational heterogeneity hinders detailed structural analysis. Molecular dynamics simulations and chemical modifications of BV42 helped us to identify a potential binding site, allowing for aptamer redesign to eliminate the conformational diversity while retaining binding affinity. Nuclear magnetic resonance spectroscopy confirmed the i-motif/duplex junction with the three-cytosine loop nearby. This study highlights the unique structural features of the functional i-motif and its role in molecular recognition of the target.Salary support from the Government project [121031300037- 7]. Academic leadership program Priority 2030 proposed by Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University).Peer reviewedOxford University PressFirst Moscow State Medical UniversityNational Research University Higher School of Economics (Russia)Tsvetkov, Vladimir [0000-0003-0494-3332]#NODATA##NODATA##NODATA##NODATA##NODATA##NODATA##NODATA##NODATA#Aralov, Andrey [0000-0003-3268-2352]González, Carlos [0000-0001-8796-1282]Zavyalova, Elena [0000-0001-5260-1973]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/380047https://api.elsevier.com/content/abstract/scopus_id/85214506988reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1093/nar/gkae1282Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3800472026-05-22T06:33:51Z
dc.title.none.fl_str_mv Unveiling the unusual i-motif-derived architecture of a DNA aptamer exhibiting high affinity for influenza A virus
title Unveiling the unusual i-motif-derived architecture of a DNA aptamer exhibiting high affinity for influenza A virus
spellingShingle Unveiling the unusual i-motif-derived architecture of a DNA aptamer exhibiting high affinity for influenza A virus
Tsvetkov, Vladimir
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
title_short Unveiling the unusual i-motif-derived architecture of a DNA aptamer exhibiting high affinity for influenza A virus
title_full Unveiling the unusual i-motif-derived architecture of a DNA aptamer exhibiting high affinity for influenza A virus
title_fullStr Unveiling the unusual i-motif-derived architecture of a DNA aptamer exhibiting high affinity for influenza A virus
title_full_unstemmed Unveiling the unusual i-motif-derived architecture of a DNA aptamer exhibiting high affinity for influenza A virus
title_sort Unveiling the unusual i-motif-derived architecture of a DNA aptamer exhibiting high affinity for influenza A virus
dc.creator.none.fl_str_mv Tsvetkov, Vladimir
Mir, Bartomeu
Alieva, Rugiya
Arutyunyan, Alexander
Oleynikov, Ilya
Novikov, Roman
Boravleva, Elizaveta
Kamzeeva, Polina
Zatsepin, Timofei
Aralov, Andrey
González, Carlos
Zavyalova, Elena
author Tsvetkov, Vladimir
author_facet Tsvetkov, Vladimir
Mir, Bartomeu
Alieva, Rugiya
Arutyunyan, Alexander
Oleynikov, Ilya
Novikov, Roman
Boravleva, Elizaveta
Kamzeeva, Polina
Zatsepin, Timofei
Aralov, Andrey
González, Carlos
Zavyalova, Elena
author_role author
author2 Mir, Bartomeu
Alieva, Rugiya
Arutyunyan, Alexander
Oleynikov, Ilya
Novikov, Roman
Boravleva, Elizaveta
Kamzeeva, Polina
Zatsepin, Timofei
Aralov, Andrey
González, Carlos
Zavyalova, Elena
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv First Moscow State Medical University
National Research University Higher School of Economics (Russia)
Tsvetkov, Vladimir [0000-0003-0494-3332]
#NODATA#
#NODATA#
#NODATA#
#NODATA#
#NODATA#
#NODATA#
#NODATA#
#NODATA#
Aralov, Andrey [0000-0003-3268-2352]
González, Carlos [0000-0001-8796-1282]
Zavyalova, Elena [0000-0001-5260-1973]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
topic http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
description 17 pages, 7 figures, 3 tables
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/380047
https://api.elsevier.com/content/abstract/scopus_id/85214506988
url http://hdl.handle.net/10261/380047
https://api.elsevier.com/content/abstract/scopus_id/85214506988
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1093/nar/gkae1282
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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