Versatile lock and key assembly for optical measurements with microfluidic platforms and cartridges

A novel and versatile optical reader for microfluidic platforms is presented. The reader includes a modular insertion port based on the lock and key concept for reproducible alignment with a miniaturized optical detection system comprising an interchangeable light emitting diode (LED) and a photodio...

Descripción completa

Detalles Bibliográficos
Autores: Ymbern Llorens, Oriol|||0000-0003-1997-6794, Berenguel-Alonso, Miguel|||0000-0002-3759-3634, Calvo-López, Antonio|||0000-0003-2728-8676, Gómez-de Pedro, Sara|||0000-0002-3794-5820, Izquierdo, David|||0000-0002-4746-3139, Alonso-Chamarro, Julián|||0000-0002-6804-6027
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:288035
Acceso en línea:https://ddd.uab.cat/record/288035
https://dx.doi.org/urn:doi:10.1021/ac504255t
Access Level:acceso abierto
Descripción
Sumario:A novel and versatile optical reader for microfluidic platforms is presented. The reader includes a modular insertion port based on the lock and key concept for reproducible alignment with a miniaturized optical detection system comprising an interchangeable light emitting diode (LED) and a photodiode. The modular nature of the insertion port allows the use of microfluidic platforms in variable shapes and fluidic configurations. Three different analytical methodologies based on absorbance or fluorescence measurements were used to demonstrate the flexibility and reproducibility of the proposed experimental setup.