In vitro long-term development of cultured inner ear stem cells of newborn rat

The adult mammalian auditory receptor lacks any ability to repair and/or regenerate after injury. However, the late developing cochlea still contains some stem-cell-like elements that might be used to regenerate damaged neurons and/or cells of the organ of Corti. Before their use in any application,...

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Detalles Bibliográficos
Autores: Carricondo Orejana, Francisco Javier, Iglesias Moreno, María Cruz, Rodríguez Gómez, Fernando Luis, Poch Broto, Joaquín, Gil Loyzaga, Pablo
Tipo de recurso: artículo
Fecha de publicación:2010
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/93741
Acceso en línea:https://hdl.handle.net/20.500.14352/93741
Access Level:acceso abierto
Palabra clave:616.21
612.017
Inner ear stem cells
Deafness
Cell culture
Spontaneous differentiation
Rat (Wistar)
Biología celular (Biología)
Otorrinolaringología
2407.01 Cultivo Celular
2407.05 Cultivo de Tejidos
2411.13 Fisiología de la Audición
3213.05 Cirugía de Garganta, Nariz y Oídos
id ES_3ebfca5ebfc6e2e44fa03f24bf817a6a
oai_identifier_str oai:docta.ucm.es:20.500.14352/93741
network_acronym_str ES
network_name_str España
repository_id_str
spelling In vitro long-term development of cultured inner ear stem cells of newborn ratCarricondo Orejana, Francisco JavierIglesias Moreno, María CruzRodríguez Gómez, Fernando LuisPoch Broto, JoaquínGil Loyzaga, Pablo616.21612.017Inner ear stem cellsDeafnessCell cultureSpontaneous differentiationRat (Wistar)Biología celular (Biología)Otorrinolaringología2407.01 Cultivo Celular2407.05 Cultivo de Tejidos2411.13 Fisiología de la Audición3213.05 Cirugía de Garganta, Nariz y OídosThe adult mammalian auditory receptor lacks any ability to repair and/or regenerate after injury. However, the late developing cochlea still contains some stem-cell-like elements that might be used to regenerate damaged neurons and/or cells of the organ of Corti. Before their use in any application, stem cell numbers need to be amplified because they are usually rare in late developing and adult tissues. The numerous re-explant cultures required for the progressive amplification process can result in a spontaneous differentiation process. This aspect has been implicated in the tumorigenicity of stem cells when transplanted into a tissue. The aim of this study has been to determine whether cochlear stem cells can proliferate and differentiate spontaneously in long-term cultures without the addition of any factor that might influence these processes. Cochlear stem cells, which express nestin protein, were cultured in monolayers and fed with DMEM containing 5% FBS. They quickly organized themselves into typical spheres exhibiting a high proliferation rate, self-renewal property, and differentiation ability. Secondary cultures of these stem cell spheres spontaneously differentiated into neuroectodermal-like cells. The expression of nestin, glial-fibrillary-acidic protein, vimentin, and neurofilaments was evaluated to identify early differentiation. Nestin expression appeared in primary and secondary cultures. Other markers were also identified in differentiating cells. Further research might demonstrate the spontaneous differentiation of cochlear stem cells and their teratogenic probability when they are used for transplantation.SpringerUniversidad Complutense de Madrid20102010-09-1420102010-09-14journal articlehttp://purl.org/coar/resource_type/c_6501CVoRhttp://purl.org/coar/version/c_e19f295774971610info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/93741reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/937412026-06-02T12:44:21Z
dc.title.none.fl_str_mv In vitro long-term development of cultured inner ear stem cells of newborn rat
title In vitro long-term development of cultured inner ear stem cells of newborn rat
spellingShingle In vitro long-term development of cultured inner ear stem cells of newborn rat
Carricondo Orejana, Francisco Javier
616.21
612.017
Inner ear stem cells
Deafness
Cell culture
Spontaneous differentiation
Rat (Wistar)
Biología celular (Biología)
Otorrinolaringología
2407.01 Cultivo Celular
2407.05 Cultivo de Tejidos
2411.13 Fisiología de la Audición
3213.05 Cirugía de Garganta, Nariz y Oídos
title_short In vitro long-term development of cultured inner ear stem cells of newborn rat
title_full In vitro long-term development of cultured inner ear stem cells of newborn rat
title_fullStr In vitro long-term development of cultured inner ear stem cells of newborn rat
title_full_unstemmed In vitro long-term development of cultured inner ear stem cells of newborn rat
title_sort In vitro long-term development of cultured inner ear stem cells of newborn rat
dc.creator.none.fl_str_mv Carricondo Orejana, Francisco Javier
Iglesias Moreno, María Cruz
Rodríguez Gómez, Fernando Luis
Poch Broto, Joaquín
Gil Loyzaga, Pablo
author Carricondo Orejana, Francisco Javier
author_facet Carricondo Orejana, Francisco Javier
Iglesias Moreno, María Cruz
Rodríguez Gómez, Fernando Luis
Poch Broto, Joaquín
Gil Loyzaga, Pablo
author_role author
author2 Iglesias Moreno, María Cruz
Rodríguez Gómez, Fernando Luis
Poch Broto, Joaquín
Gil Loyzaga, Pablo
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 616.21
612.017
Inner ear stem cells
Deafness
Cell culture
Spontaneous differentiation
Rat (Wistar)
Biología celular (Biología)
Otorrinolaringología
2407.01 Cultivo Celular
2407.05 Cultivo de Tejidos
2411.13 Fisiología de la Audición
3213.05 Cirugía de Garganta, Nariz y Oídos
topic 616.21
612.017
Inner ear stem cells
Deafness
Cell culture
Spontaneous differentiation
Rat (Wistar)
Biología celular (Biología)
Otorrinolaringología
2407.01 Cultivo Celular
2407.05 Cultivo de Tejidos
2411.13 Fisiología de la Audición
3213.05 Cirugía de Garganta, Nariz y Oídos
description The adult mammalian auditory receptor lacks any ability to repair and/or regenerate after injury. However, the late developing cochlea still contains some stem-cell-like elements that might be used to regenerate damaged neurons and/or cells of the organ of Corti. Before their use in any application, stem cell numbers need to be amplified because they are usually rare in late developing and adult tissues. The numerous re-explant cultures required for the progressive amplification process can result in a spontaneous differentiation process. This aspect has been implicated in the tumorigenicity of stem cells when transplanted into a tissue. The aim of this study has been to determine whether cochlear stem cells can proliferate and differentiate spontaneously in long-term cultures without the addition of any factor that might influence these processes. Cochlear stem cells, which express nestin protein, were cultured in monolayers and fed with DMEM containing 5% FBS. They quickly organized themselves into typical spheres exhibiting a high proliferation rate, self-renewal property, and differentiation ability. Secondary cultures of these stem cell spheres spontaneously differentiated into neuroectodermal-like cells. The expression of nestin, glial-fibrillary-acidic protein, vimentin, and neurofilaments was evaluated to identify early differentiation. Nestin expression appeared in primary and secondary cultures. Other markers were also identified in differentiating cells. Further research might demonstrate the spontaneous differentiation of cochlear stem cells and their teratogenic probability when they are used for transplantation.
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-09-14
2010
2010-09-14
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
CVoR
http://purl.org/coar/version/c_e19f295774971610
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/93741
url https://hdl.handle.net/20.500.14352/93741
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869406573690880000
score 15,300724

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