Rapid amplitude-modulation of a diarylethene photoswitch: en route to contrast-enhanced fluorescence imaging

A water soluble diarylethene (DAE) derivative that displays exceptionally intense fluorescence from the colorless open form has been synthesized and characterized using UV/vis spectroscopy and fluorescence microscopy. We show that the bright emission from the open form can be rapidly switched using...

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Detalhes bibliográficos
Autores: Naren, Gaowa, Larsson, Wera, Benitez-Martin, Carlos, Li, Shiming, Pérez-Inestrosa, Ezequiel, Albinsson, Bo, Andréasson, Joakim
Formato: artículo
Fecha de publicación:2021
País:España
Recursos:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/18311
Acesso em linha:http://hdl.handle.net/20.500.12105/18311
Access Level:acceso abierto
Palavra-chave:Fluorescence
Microscopy, fluorescence
Fluorescent dyes
UV
Fluorescencia
Microscopía fluorescente
Colorantes fluorescentes
Rayos ultravioleta
Microscopy, Fluorescence
Spectrometry, Fluorescence
Fluorescent Dyes
Solubility
Light
Ultraviolet Rays
Microscopy
Cell Culture Techniques
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oai_identifier_str oai:repisalud.isciii.es:20.500.12105/18311
network_acronym_str ES
network_name_str España
repository_id_str
spelling Rapid amplitude-modulation of a diarylethene photoswitch: en route to contrast-enhanced fluorescence imagingNaren, GaowaLarsson, WeraBenitez-Martin, CarlosLi, ShimingPérez-Inestrosa, EzequielAlbinsson, BoAndréasson, JoakimFluorescenceMicroscopy, fluorescenceFluorescent dyesUVFluorescenciaMicroscopía fluorescenteColorantes fluorescentesRayos ultravioletaMicroscopy, FluorescenceSpectrometry, FluorescenceFluorescent DyesSolubilityLightUltraviolet RaysMicroscopyCell Culture TechniquesA water soluble diarylethene (DAE) derivative that displays exceptionally intense fluorescence from the colorless open form has been synthesized and characterized using UV/vis spectroscopy and fluorescence microscopy. We show that the bright emission from the open form can be rapidly switched using amplitude modulated red light, that is, by light at wavelengths longer than those absorbed by the fluorescent species. This is highly appealing in any context where undesired background fluorescence disturbs the measurement, e.g., the autofluorescence commonly observed in fluorescence microscopy. We show that this scheme is conveniently applicable using lock-in detection, and that robust amplitude modulation of the probe fluorescence is indeed possible also in cell studies using fluorescence microscopy.Royal Society of Chemistry (RSC)[Naren,G; Larsson,W; Li,S; Albinsson,B; Andréasson,J] Chemistry and Chemical Engineering, Chemistry and Biochemistry, Chalmers University of Technology, Göteborg, Sweden. [Benitez-Martin,C; Pérez-Inestrosa,E] Universidad de Malaga-IBIMA, Departamento de Química Organica, E-29071 Málaga, Spain. [Benitez-Martin,C; Pérez-Inestrosa,E] Centro Andaluz de Nanomedicina y Biotecnología (BIONAND), Malaga, Spain.20242024-02-1920212021-04-1520212021-04-15research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://hdl.handle.net/20.500.12105/18311reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/183112026-06-12T12:43:37Z
dc.title.none.fl_str_mv Rapid amplitude-modulation of a diarylethene photoswitch: en route to contrast-enhanced fluorescence imaging
title Rapid amplitude-modulation of a diarylethene photoswitch: en route to contrast-enhanced fluorescence imaging
spellingShingle Rapid amplitude-modulation of a diarylethene photoswitch: en route to contrast-enhanced fluorescence imaging
Naren, Gaowa
Fluorescence
Microscopy, fluorescence
Fluorescent dyes
UV
Fluorescencia
Microscopía fluorescente
Colorantes fluorescentes
Rayos ultravioleta
Microscopy, Fluorescence
Spectrometry, Fluorescence
Fluorescent Dyes
Solubility
Light
Ultraviolet Rays
Microscopy
Cell Culture Techniques
title_short Rapid amplitude-modulation of a diarylethene photoswitch: en route to contrast-enhanced fluorescence imaging
title_full Rapid amplitude-modulation of a diarylethene photoswitch: en route to contrast-enhanced fluorescence imaging
title_fullStr Rapid amplitude-modulation of a diarylethene photoswitch: en route to contrast-enhanced fluorescence imaging
title_full_unstemmed Rapid amplitude-modulation of a diarylethene photoswitch: en route to contrast-enhanced fluorescence imaging
title_sort Rapid amplitude-modulation of a diarylethene photoswitch: en route to contrast-enhanced fluorescence imaging
dc.creator.none.fl_str_mv Naren, Gaowa
Larsson, Wera
Benitez-Martin, Carlos
Li, Shiming
Pérez-Inestrosa, Ezequiel
Albinsson, Bo
Andréasson, Joakim
author Naren, Gaowa
author_facet Naren, Gaowa
Larsson, Wera
Benitez-Martin, Carlos
Li, Shiming
Pérez-Inestrosa, Ezequiel
Albinsson, Bo
Andréasson, Joakim
author_role author
author2 Larsson, Wera
Benitez-Martin, Carlos
Li, Shiming
Pérez-Inestrosa, Ezequiel
Albinsson, Bo
Andréasson, Joakim
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv [Naren,G; Larsson,W; Li,S; Albinsson,B; Andréasson,J] Chemistry and Chemical Engineering, Chemistry and Biochemistry, Chalmers University of Technology, Göteborg, Sweden. [Benitez-Martin,C; Pérez-Inestrosa,E] Universidad de Malaga-IBIMA, Departamento de Química Organica, E-29071 Málaga, Spain. [Benitez-Martin,C; Pérez-Inestrosa,E] Centro Andaluz de Nanomedicina y Biotecnología (BIONAND), Malaga, Spain.
dc.subject.none.fl_str_mv Fluorescence
Microscopy, fluorescence
Fluorescent dyes
UV
Fluorescencia
Microscopía fluorescente
Colorantes fluorescentes
Rayos ultravioleta
Microscopy, Fluorescence
Spectrometry, Fluorescence
Fluorescent Dyes
Solubility
Light
Ultraviolet Rays
Microscopy
Cell Culture Techniques
topic Fluorescence
Microscopy, fluorescence
Fluorescent dyes
UV
Fluorescencia
Microscopía fluorescente
Colorantes fluorescentes
Rayos ultravioleta
Microscopy, Fluorescence
Spectrometry, Fluorescence
Fluorescent Dyes
Solubility
Light
Ultraviolet Rays
Microscopy
Cell Culture Techniques
description A water soluble diarylethene (DAE) derivative that displays exceptionally intense fluorescence from the colorless open form has been synthesized and characterized using UV/vis spectroscopy and fluorescence microscopy. We show that the bright emission from the open form can be rapidly switched using amplitude modulated red light, that is, by light at wavelengths longer than those absorbed by the fluorescent species. This is highly appealing in any context where undesired background fluorescence disturbs the measurement, e.g., the autofluorescence commonly observed in fluorescence microscopy. We show that this scheme is conveniently applicable using lock-in detection, and that robust amplitude modulation of the probe fluorescence is indeed possible also in cell studies using fluorescence microscopy.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-04-15
2021
2021-04-15
2024
2024-02-19
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/18311
url http://hdl.handle.net/20.500.12105/18311
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Royal Society of Chemistry (RSC)
publisher.none.fl_str_mv Royal Society of Chemistry (RSC)
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869406542521958400
score 15,812429

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