Tomato geranylgeranyl diphosphate synthase isoform 1 is involved in the stress-triggered production of diterpenes in leaves and strigolactones in roots

Carotenoids are photoprotectant pigments and precursors of hormones such as strigolactones (SL). Carotenoids are produced in plastids from geranylgeranyl diphosphate (GGPP), which is diverted to the carotenoid pathway by phytoene synthase (PSY). In tomato (Solanum lycopersicum), three genes encode p...

Descripción completa

Detalles Bibliográficos
Autores: Ezquerro, Miguel, Li, Changsheng, Pérez-Pérez, Julia, Burbano-Erazo, Esteban, Barja, M. Victoria, Wang, Yanting, Dong, Lemeng, Lisón, Purificación, López-Gresa, M Pilar, Bouwmeester, Harro
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/340775
Acceso en línea:http://hdl.handle.net/10261/340775
https://api.elsevier.com/content/abstract/scopus_id/85164194462
Access Level:acceso abierto
Palabra clave:Abscisic acid (ABA)
Carotenoid
Diterpenes
Geranylgeranyl diphosphate (GGPP)
Phytoene synthase
Strigolactones
id ES_3e5434181d4796d64f5ca8d1a734da2e
oai_identifier_str oai:digital.csic.es:10261/340775
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Tomato geranylgeranyl diphosphate synthase isoform 1 is involved in the stress-triggered production of diterpenes in leaves and strigolactones in roots
title Tomato geranylgeranyl diphosphate synthase isoform 1 is involved in the stress-triggered production of diterpenes in leaves and strigolactones in roots
spellingShingle Tomato geranylgeranyl diphosphate synthase isoform 1 is involved in the stress-triggered production of diterpenes in leaves and strigolactones in roots
Ezquerro, Miguel
Abscisic acid (ABA)
Carotenoid
Diterpenes
Geranylgeranyl diphosphate (GGPP)
Phytoene synthase
Strigolactones
title_short Tomato geranylgeranyl diphosphate synthase isoform 1 is involved in the stress-triggered production of diterpenes in leaves and strigolactones in roots
title_full Tomato geranylgeranyl diphosphate synthase isoform 1 is involved in the stress-triggered production of diterpenes in leaves and strigolactones in roots
title_fullStr Tomato geranylgeranyl diphosphate synthase isoform 1 is involved in the stress-triggered production of diterpenes in leaves and strigolactones in roots
title_full_unstemmed Tomato geranylgeranyl diphosphate synthase isoform 1 is involved in the stress-triggered production of diterpenes in leaves and strigolactones in roots
title_sort Tomato geranylgeranyl diphosphate synthase isoform 1 is involved in the stress-triggered production of diterpenes in leaves and strigolactones in roots
dc.creator.none.fl_str_mv Ezquerro, Miguel
Li, Changsheng
Pérez-Pérez, Julia
Burbano-Erazo, Esteban
Barja, M. Victoria
Wang, Yanting
Dong, Lemeng
Lisón, Purificación
López-Gresa, M Pilar
Bouwmeester, Harro
author Ezquerro, Miguel
author_facet Ezquerro, Miguel
Li, Changsheng
Pérez-Pérez, Julia
Burbano-Erazo, Esteban
Barja, M. Victoria
Wang, Yanting
Dong, Lemeng
Lisón, Purificación
López-Gresa, M Pilar
Bouwmeester, Harro
author_role author
author2 Li, Changsheng
Pérez-Pérez, Julia
Burbano-Erazo, Esteban
Barja, M. Victoria
Wang, Yanting
Dong, Lemeng
Lisón, Purificación
López-Gresa, M Pilar
Bouwmeester, Harro
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Generalitat Valenciana
EMBO
Ezquerro, Miguel [0000-0002-3051-5502]
Li, Changsheng [0000-0002-9579-4127]
Pérez-Pérez, Julia [0000-0001-9448-0700]
Burbano-Erazo, Esteban [0000-0001-5056-9893]
Barja, M Victoria [0000-0002-3846-4885]
Wang, Yanting [0000-0003-1970-4158]
Dong, Lemeng [0000-0002-1435-1907]
Lisón, Purificación [0000-0002-1662-8084]
López-Gresa, M Pilar [0000-0001-9251-0160]
Bouwmeester, Harro [0000-0003-0907-2732]
Rodríguez-Concepción, Manuel [0000-0002-1280-2305]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Abscisic acid (ABA)
Carotenoid
Diterpenes
Geranylgeranyl diphosphate (GGPP)
Phytoene synthase
Strigolactones
topic Abscisic acid (ABA)
Carotenoid
Diterpenes
Geranylgeranyl diphosphate (GGPP)
Phytoene synthase
Strigolactones
description Carotenoids are photoprotectant pigments and precursors of hormones such as strigolactones (SL). Carotenoids are produced in plastids from geranylgeranyl diphosphate (GGPP), which is diverted to the carotenoid pathway by phytoene synthase (PSY). In tomato (Solanum lycopersicum), three genes encode plastid-targeted GGPP synthases (SlG1 to SlG3) and three genes encode PSY isoforms (PSY1 to PSY3). Here, we investigated the function of SlG1 by generating loss-of-function lines and combining their metabolic and physiological phenotyping with gene co-expression and co-immunoprecipitation analyses. Leaves and fruits of slg1 lines showed a wild-type phenotype in terms of carotenoid accumulation, photosynthesis, and development under normal growth conditions. In response to bacterial infection, however, slg1 leaves produced lower levels of defensive GGPP-derived diterpenoids. In roots, SlG1 was co-expressed with PSY3 and other genes involved in SL production, and slg1 lines grown under phosphate starvation exuded less SLs. However, slg1 plants did not display the branched shoot phenotype observed in other SL-defective mutants. At the protein level, SlG1 physically interacted with the root-specific PSY3 isoform but not with PSY1 and PSY2. Our results confirm specific roles for SlG1 in producing GGPP for defensive diterpenoids in leaves and carotenoid-derived SLs (in combination with PSY3) in roots.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/340775
https://api.elsevier.com/content/abstract/scopus_id/85164194462
url http://hdl.handle.net/10261/340775
https://api.elsevier.com/content/abstract/scopus_id/85164194462
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115810GB-I00
info:eu-repo/grantAgreement/MINECO//SEV-2015-0533-17-1
Ezquerro, Miguel; Li, Changsheng; Pérez-Pérez, Julia; Burbano-Erazo, Esteban; Barja, M Victoria; Wang, Yanting; Dong, Lemeng; Lisón, Purificación; López-Gresa, M Pilar; Bouwmeester, Harro; 2023; Supporting Information of the article Tomato geranylgeranyl diphosphate synthase isoform 1 is involved in the stress-triggered production of diterpenes in leaves and strigolactones in roots [Dataset]; John Wiley & Sons; https://doi.org/10.1111/nph.19109
https://doi.org/10.1111/nph.19109
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869406525909368832
spelling Tomato geranylgeranyl diphosphate synthase isoform 1 is involved in the stress-triggered production of diterpenes in leaves and strigolactones in rootsEzquerro, MiguelLi, ChangshengPérez-Pérez, JuliaBurbano-Erazo, EstebanBarja, M. VictoriaWang, YantingDong, LemengLisón, PurificaciónLópez-Gresa, M PilarBouwmeester, HarroAbscisic acid (ABA)CarotenoidDiterpenesGeranylgeranyl diphosphate (GGPP)Phytoene synthaseStrigolactonesCarotenoids are photoprotectant pigments and precursors of hormones such as strigolactones (SL). Carotenoids are produced in plastids from geranylgeranyl diphosphate (GGPP), which is diverted to the carotenoid pathway by phytoene synthase (PSY). In tomato (Solanum lycopersicum), three genes encode plastid-targeted GGPP synthases (SlG1 to SlG3) and three genes encode PSY isoforms (PSY1 to PSY3). Here, we investigated the function of SlG1 by generating loss-of-function lines and combining their metabolic and physiological phenotyping with gene co-expression and co-immunoprecipitation analyses. Leaves and fruits of slg1 lines showed a wild-type phenotype in terms of carotenoid accumulation, photosynthesis, and development under normal growth conditions. In response to bacterial infection, however, slg1 leaves produced lower levels of defensive GGPP-derived diterpenoids. In roots, SlG1 was co-expressed with PSY3 and other genes involved in SL production, and slg1 lines grown under phosphate starvation exuded less SLs. However, slg1 plants did not display the branched shoot phenotype observed in other SL-defective mutants. At the protein level, SlG1 physically interacted with the root-specific PSY3 isoform but not with PSY1 and PSY2. Our results confirm specific roles for SlG1 in producing GGPP for defensive diterpenoids in leaves and carotenoid-derived SLs (in combination with PSY3) in roots.This work was funded by grants from Spanish MCIN/AEI/10.13039/501100011033 and European NextGeneration EU/PRTR and PRIMA programs to MR-C (PID2020-115810GB-I00 and UToPIQ-PCI2021-121941). Funding was also provided by Generalitat Valenciana (PROMETEU/2021/056 and AGROALNEXT/2022/067). ME received a predoctoral fellowship from MCIN/AEI (BES-2017-080652) and an EMBO Scientific Exchange Grant (9315).Peer reviewedJohn Wiley & SonsEuropean CommissionMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Generalitat ValencianaEMBOEzquerro, Miguel [0000-0002-3051-5502]Li, Changsheng [0000-0002-9579-4127]Pérez-Pérez, Julia [0000-0001-9448-0700]Burbano-Erazo, Esteban [0000-0001-5056-9893]Barja, M Victoria [0000-0002-3846-4885]Wang, Yanting [0000-0003-1970-4158]Dong, Lemeng [0000-0002-1435-1907]Lisón, Purificación [0000-0002-1662-8084]López-Gresa, M Pilar [0000-0001-9251-0160]Bouwmeester, Harro [0000-0003-0907-2732]Rodríguez-Concepción, Manuel [0000-0002-1280-2305]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/340775https://api.elsevier.com/content/abstract/scopus_id/85164194462reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115810GB-I00info:eu-repo/grantAgreement/MINECO//SEV-2015-0533-17-1Ezquerro, Miguel; Li, Changsheng; Pérez-Pérez, Julia; Burbano-Erazo, Esteban; Barja, M Victoria; Wang, Yanting; Dong, Lemeng; Lisón, Purificación; López-Gresa, M Pilar; Bouwmeester, Harro; 2023; Supporting Information of the article Tomato geranylgeranyl diphosphate synthase isoform 1 is involved in the stress-triggered production of diterpenes in leaves and strigolactones in roots [Dataset]; John Wiley & Sons; https://doi.org/10.1111/nph.19109https://doi.org/10.1111/nph.19109Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3407752026-05-22T06:33:51Z
score 15,81155

Similares en LA Referencia