Gut bacterial ClpB-like gene function is associated with decreased body weight and a characteristic microbiota profile

Background The chaperone ClpB, a bacterial protein, is a conformational antigen-mimetic of α-melanocyte-stimulating hormone (α-MSH) implicated in body weight regulation in mice. We here investigated the potential associations of gut bacterial ClpB-like gene function with obesity status and gut micro...

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Autores: Arnoriaga Rodríguez, María, Mayneris Perxachs, Jordi, Burokas, Aurelijus, Pérez Brocal, Vicente, Moya, Andrés, Portero Otin, Manuel, Ricart, Wifredo, Maldonado, Rafael, Fernández-Real Lemos, José Manuel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/18083
Acceso en línea:http://hdl.handle.net/10256/18083
Access Level:acceso abierto
Palabra clave:Intestins -- Microbiologia
Intestines -- Microbiology
Intestins -- Malalties
Intestines -- Diseases
Persones obeses
Overweight persons
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network_acronym_str ES
network_name_str España
repository_id_str
spelling Gut bacterial ClpB-like gene function is associated with decreased body weight and a characteristic microbiota profileArnoriaga Rodríguez, MaríaMayneris Perxachs, JordiBurokas, AurelijusPérez Brocal, VicenteMoya, AndrésPortero Otin, ManuelRicart, WifredoMaldonado, RafaelFernández-Real Lemos, José ManuelIntestins -- MicrobiologiaIntestines -- MicrobiologyIntestins -- MalaltiesIntestines -- DiseasesPersones obesesOverweight personsBackground The chaperone ClpB, a bacterial protein, is a conformational antigen-mimetic of α-melanocyte-stimulating hormone (α-MSH) implicated in body weight regulation in mice. We here investigated the potential associations of gut bacterial ClpB-like gene function with obesity status and gut microbiota in humans. Results Gut microbiota ClpB KEGG function was negatively associated with body mass index, waist circumference, and total fat mass (DEXA). The relative abundance (RA) of several phyla and families directly associated with ClpB was decreased in subjects with obesity. Specifically, the RA of Rikenellaceae, Clostridiaceae and not assigned Firmicutes were lower in subjects with obesity and positively associated with gut bacterial ClpB-like gene function (not assigned Firmicutes (r = 0.405, FDR = 2.93 × 10−2), Rikenellaceae (r = 0.217, FDR = 0.031), and Clostridiaceae (r = 0.239, FDR = 0.017)). The gut bacterial ClpB-like gene function was also linked to specific plasma metabolites (hippuric acid and 3-indolepropionic acid) and fecal lupeol. The α-MSH-like epitope similar to that of Escherichia coli ClpB was also identified in some sequences of those bacterial families. After fecal transplantation from humans to mice, the families that more contributed to ClpB-like gene function in humans were also associated with ClpB-like gene function in mice after adjusting for the donor's body mass index (not assigned Firmicutes (r = 0.621, p = 0.003), Prevotellaceae (r = 0.725, p = 4.1 × 10−7), Rikenellaceae (r = 0.702, p = 3.9 × 10−4), and Ruminococcaceae (r = 0.526, p = 0.014)). Clostridiaceae (r = − 0.445, p = 0.038) and Prevotellaceae RA (r = − 0.479, p = 0.024) and were also negatively associated with weight gain in mice. The absolute abundance (AA) of Prevotellaceae in mice was also positively associated with the gut bacterial ClpB-like gene function in mice. DESeq2 identified species of Prevotellaceae, both negatively associated with mice' weight gain and positively with gut bacterial ClpB-like gene function. Conclusions In summary, gut bacterial ClpB-like gene function is associated with obesity status, a specific gut microbiota composition and a plasma metabolomics profile in humans that could be partially transplanted to miceBioMed Central2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer-reviewedapplication/pdfhttp://hdl.handle.net/10256/18083Microbiome, 2020, vol. 8, art.núm. 59Articles publicats (D-CM)Arnoriaga Rodríguez, María Mayneris Perxchas, Jordi Burokas, Aurelijus Pérez Brocal, Vicente Moya, Andrés Portero Otin, Manuel Ricart, Wifredo Maldonado, Rafael Fernández-Real Lemos, José Manuel 2020 Gut bacterial ClpB-like gene function is associated with decreased body weight and a characteristic microbiota profile Microbiome 8 1 59reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1186/s40168-020-00837-6info:eu-repo/semantics/altIdentifier/issn/2049-2618Reconeixement 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:recercat.cat:10256/180832026-05-29T05:05:01Z
dc.title.none.fl_str_mv Gut bacterial ClpB-like gene function is associated with decreased body weight and a characteristic microbiota profile
title Gut bacterial ClpB-like gene function is associated with decreased body weight and a characteristic microbiota profile
spellingShingle Gut bacterial ClpB-like gene function is associated with decreased body weight and a characteristic microbiota profile
Arnoriaga Rodríguez, María
Intestins -- Microbiologia
Intestines -- Microbiology
Intestins -- Malalties
Intestines -- Diseases
Persones obeses
Overweight persons
title_short Gut bacterial ClpB-like gene function is associated with decreased body weight and a characteristic microbiota profile
title_full Gut bacterial ClpB-like gene function is associated with decreased body weight and a characteristic microbiota profile
title_fullStr Gut bacterial ClpB-like gene function is associated with decreased body weight and a characteristic microbiota profile
title_full_unstemmed Gut bacterial ClpB-like gene function is associated with decreased body weight and a characteristic microbiota profile
title_sort Gut bacterial ClpB-like gene function is associated with decreased body weight and a characteristic microbiota profile
dc.creator.none.fl_str_mv Arnoriaga Rodríguez, María
Mayneris Perxachs, Jordi
Burokas, Aurelijus
Pérez Brocal, Vicente
Moya, Andrés
Portero Otin, Manuel
Ricart, Wifredo
Maldonado, Rafael
Fernández-Real Lemos, José Manuel
author Arnoriaga Rodríguez, María
author_facet Arnoriaga Rodríguez, María
Mayneris Perxachs, Jordi
Burokas, Aurelijus
Pérez Brocal, Vicente
Moya, Andrés
Portero Otin, Manuel
Ricart, Wifredo
Maldonado, Rafael
Fernández-Real Lemos, José Manuel
author_role author
author2 Mayneris Perxachs, Jordi
Burokas, Aurelijus
Pérez Brocal, Vicente
Moya, Andrés
Portero Otin, Manuel
Ricart, Wifredo
Maldonado, Rafael
Fernández-Real Lemos, José Manuel
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Intestins -- Microbiologia
Intestines -- Microbiology
Intestins -- Malalties
Intestines -- Diseases
Persones obeses
Overweight persons
topic Intestins -- Microbiologia
Intestines -- Microbiology
Intestins -- Malalties
Intestines -- Diseases
Persones obeses
Overweight persons
description Background The chaperone ClpB, a bacterial protein, is a conformational antigen-mimetic of α-melanocyte-stimulating hormone (α-MSH) implicated in body weight regulation in mice. We here investigated the potential associations of gut bacterial ClpB-like gene function with obesity status and gut microbiota in humans. Results Gut microbiota ClpB KEGG function was negatively associated with body mass index, waist circumference, and total fat mass (DEXA). The relative abundance (RA) of several phyla and families directly associated with ClpB was decreased in subjects with obesity. Specifically, the RA of Rikenellaceae, Clostridiaceae and not assigned Firmicutes were lower in subjects with obesity and positively associated with gut bacterial ClpB-like gene function (not assigned Firmicutes (r = 0.405, FDR = 2.93 × 10−2), Rikenellaceae (r = 0.217, FDR = 0.031), and Clostridiaceae (r = 0.239, FDR = 0.017)). The gut bacterial ClpB-like gene function was also linked to specific plasma metabolites (hippuric acid and 3-indolepropionic acid) and fecal lupeol. The α-MSH-like epitope similar to that of Escherichia coli ClpB was also identified in some sequences of those bacterial families. After fecal transplantation from humans to mice, the families that more contributed to ClpB-like gene function in humans were also associated with ClpB-like gene function in mice after adjusting for the donor's body mass index (not assigned Firmicutes (r = 0.621, p = 0.003), Prevotellaceae (r = 0.725, p = 4.1 × 10−7), Rikenellaceae (r = 0.702, p = 3.9 × 10−4), and Ruminococcaceae (r = 0.526, p = 0.014)). Clostridiaceae (r = − 0.445, p = 0.038) and Prevotellaceae RA (r = − 0.479, p = 0.024) and were also negatively associated with weight gain in mice. The absolute abundance (AA) of Prevotellaceae in mice was also positively associated with the gut bacterial ClpB-like gene function in mice. DESeq2 identified species of Prevotellaceae, both negatively associated with mice' weight gain and positively with gut bacterial ClpB-like gene function. Conclusions In summary, gut bacterial ClpB-like gene function is associated with obesity status, a specific gut microbiota composition and a plasma metabolomics profile in humans that could be partially transplanted to mice
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer-reviewed
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/18083
url http://hdl.handle.net/10256/18083
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/s40168-020-00837-6
info:eu-repo/semantics/altIdentifier/issn/2049-2618
dc.rights.none.fl_str_mv Reconeixement 4.0 Internacional
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Reconeixement 4.0 Internacional
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Microbiome, 2020, vol. 8, art.núm. 59
Articles publicats (D-CM)
Arnoriaga Rodríguez, María Mayneris Perxchas, Jordi Burokas, Aurelijus Pérez Brocal, Vicente Moya, Andrés Portero Otin, Manuel Ricart, Wifredo Maldonado, Rafael Fernández-Real Lemos, José Manuel 2020 Gut bacterial ClpB-like gene function is associated with decreased body weight and a characteristic microbiota profile Microbiome 8 1 59
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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