A novel combined scientific and artistic approach for advanced characterization of interactomes:
The main objective of this study was to propose a novel methodology to approach challenges in molecular biology. Akirin/Subolesin (AKR/SUB) are vaccine protective antigens and are a model for the study of the interactome due to its conserved function in the regulation of different biological process...
| Autores: | , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Universidad de Castilla-La Mancha |
| Repositorio: | RUIdeRA. Repositorio Institucional de la UCLM |
| OAI Identifier: | oai:ruidera.uclm.es:10578/27383 |
| Acceso en línea: | http://hdl.handle.net/10578/27383 |
| Access Level: | acceso abierto |
| Palabra clave: | akirin subolesin interactome art evolution music yeast two-hybrid NF-kB vaccine quantum vaccinomics protective epitope |
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A novel combined scientific and artistic approach for advanced characterization of interactomes:the Akirin/Subolesin modelArtigas Jerónimo, SaraPastor Comín, Juan JoséVillar Rayo, Margarita MaríaContreras Rojo, MarinelaAlberdi Vélez, María PilarLeón Viera, IsraelSoto, LeandroCordero, RaúlValdés, James J.Cabezas Cruz, AlejandroEstrada Peña, AgustínFuente García, José de Jesús de laakirinsubolesininteractomeartevolutionmusicyeast two-hybridNF-kBvaccinequantum vaccinomicsprotective epitopeThe main objective of this study was to propose a novel methodology to approach challenges in molecular biology. Akirin/Subolesin (AKR/SUB) are vaccine protective antigens and are a model for the study of the interactome due to its conserved function in the regulation of different biological processes such as immunity and development throughout the metazoan. Herein, three visual artists and a music professor collaborated with scientists for the functional characterization of the AKR2 interactome in the regulation of the NF-?B pathway in human placenta cells. The results served as a methodological proof-of-concept to advance this research area. The results showed new perspectives on unexplored characteristics of AKR2 with functional implications. These results included protein dimerization, the physical interactions with different proteins simultaneously to regulate various biological processes defined by cell type-specific AKR-protein interactions, and how these interactions positively or negatively regulate the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-?B) signaling pathway in a biological context-dependent manner. These results suggested that AKR2-interacting proteins might constitute suitable secondary transcription factors for cell- and stimulus-specific regulation of NF-?B. Musical perspective supported AKR/SUB evolutionary conservation in different species and provided new mechanistic insights into the AKR2 interactome. The combined scientific and artistic perspectives resulted in a multidisciplinary approach, advancing our knowledge on AKR/SUB interactome, and provided new insights into the function of AKR2-protein interactions in the regulation of the NF-?B pathway. Additionally, herein we proposed an algorithm for quantum vaccinomics by focusing on the model proteins AKR/SUB.MDPI202120212020info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/10578/27383reponame:RUIdeRA. Repositorio Institucional de la UCLMinstname:Universidad de Castilla-La ManchaInglésinfo:eu-repo/semantics/openAccessoai:ruidera.uclm.es:10578/273832026-05-27T07:36:41Z |
| dc.title.none.fl_str_mv |
A novel combined scientific and artistic approach for advanced characterization of interactomes: the Akirin/Subolesin model |
| title |
A novel combined scientific and artistic approach for advanced characterization of interactomes: |
| spellingShingle |
A novel combined scientific and artistic approach for advanced characterization of interactomes: Artigas Jerónimo, Sara akirin subolesin interactome art evolution music yeast two-hybrid NF-kB vaccine quantum vaccinomics protective epitope |
| title_short |
A novel combined scientific and artistic approach for advanced characterization of interactomes: |
| title_full |
A novel combined scientific and artistic approach for advanced characterization of interactomes: |
| title_fullStr |
A novel combined scientific and artistic approach for advanced characterization of interactomes: |
| title_full_unstemmed |
A novel combined scientific and artistic approach for advanced characterization of interactomes: |
| title_sort |
A novel combined scientific and artistic approach for advanced characterization of interactomes: |
| dc.creator.none.fl_str_mv |
Artigas Jerónimo, Sara Pastor Comín, Juan José Villar Rayo, Margarita María Contreras Rojo, Marinela Alberdi Vélez, María Pilar León Viera, Israel Soto, Leandro Cordero, Raúl Valdés, James J. Cabezas Cruz, Alejandro Estrada Peña, Agustín Fuente García, José de Jesús de la |
| author |
Artigas Jerónimo, Sara |
| author_facet |
Artigas Jerónimo, Sara Pastor Comín, Juan José Villar Rayo, Margarita María Contreras Rojo, Marinela Alberdi Vélez, María Pilar León Viera, Israel Soto, Leandro Cordero, Raúl Valdés, James J. Cabezas Cruz, Alejandro Estrada Peña, Agustín Fuente García, José de Jesús de la |
| author_role |
author |
| author2 |
Pastor Comín, Juan José Villar Rayo, Margarita María Contreras Rojo, Marinela Alberdi Vélez, María Pilar León Viera, Israel Soto, Leandro Cordero, Raúl Valdés, James J. Cabezas Cruz, Alejandro Estrada Peña, Agustín Fuente García, José de Jesús de la |
| author2_role |
author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
akirin subolesin interactome art evolution music yeast two-hybrid NF-kB vaccine quantum vaccinomics protective epitope |
| topic |
akirin subolesin interactome art evolution music yeast two-hybrid NF-kB vaccine quantum vaccinomics protective epitope |
| description |
The main objective of this study was to propose a novel methodology to approach challenges in molecular biology. Akirin/Subolesin (AKR/SUB) are vaccine protective antigens and are a model for the study of the interactome due to its conserved function in the regulation of different biological processes such as immunity and development throughout the metazoan. Herein, three visual artists and a music professor collaborated with scientists for the functional characterization of the AKR2 interactome in the regulation of the NF-?B pathway in human placenta cells. The results served as a methodological proof-of-concept to advance this research area. The results showed new perspectives on unexplored characteristics of AKR2 with functional implications. These results included protein dimerization, the physical interactions with different proteins simultaneously to regulate various biological processes defined by cell type-specific AKR-protein interactions, and how these interactions positively or negatively regulate the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-?B) signaling pathway in a biological context-dependent manner. These results suggested that AKR2-interacting proteins might constitute suitable secondary transcription factors for cell- and stimulus-specific regulation of NF-?B. Musical perspective supported AKR/SUB evolutionary conservation in different species and provided new mechanistic insights into the AKR2 interactome. The combined scientific and artistic perspectives resulted in a multidisciplinary approach, advancing our knowledge on AKR/SUB interactome, and provided new insights into the function of AKR2-protein interactions in the regulation of the NF-?B pathway. Additionally, herein we proposed an algorithm for quantum vaccinomics by focusing on the model proteins AKR/SUB. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020 2021 2021 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10578/27383 |
| url |
http://hdl.handle.net/10578/27383 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
MDPI |
| publisher.none.fl_str_mv |
MDPI |
| dc.source.none.fl_str_mv |
reponame:RUIdeRA. Repositorio Institucional de la UCLM instname:Universidad de Castilla-La Mancha |
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Universidad de Castilla-La Mancha |
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RUIdeRA. Repositorio Institucional de la UCLM |
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RUIdeRA. Repositorio Institucional de la UCLM |
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1869406522488913920 |
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15.300719 |