Microbial diversity dynamics in a methanogenic-sulfidogenic UASB reactor

In this study, the long-term performance and microbial dynamics of an Upflow Anaerobic Sludge Blanket (UASB) reactor targeting sulfate reduction in a SOx emissions treatment system were assessed using crude glycerol as organic carbon source and electron donor under constant S and C loading rates. Th...

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Autores: Fernandez Palacios, Eva|||0000-0002-0095-220X, Zhou, Xudong, Mora Garrido, Mabel|||0000-0002-2979-3601, Gabriel, David|||0000-0002-7713-4192
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:236673
Acceso en línea:https://ddd.uab.cat/record/236673
https://dx.doi.org/urn:doi:10.3390/ijerph18031305
Access Level:acceso abierto
Palabra clave:Bioscrubber
Crude glycerol
UASB
Sulfate-reducing bacteria
Microbial diversity shifts
Sequencing
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spelling Microbial diversity dynamics in a methanogenic-sulfidogenic UASB reactorFernandez Palacios, Eva|||0000-0002-0095-220XZhou, XudongMora Garrido, Mabel|||0000-0002-2979-3601Gabriel, David|||0000-0002-7713-4192BioscrubberCrude glycerolUASBSulfate-reducing bacteriaMicrobial diversity shiftsSequencingIn this study, the long-term performance and microbial dynamics of an Upflow Anaerobic Sludge Blanket (UASB) reactor targeting sulfate reduction in a SOx emissions treatment system were assessed using crude glycerol as organic carbon source and electron donor under constant S and C loading rates. The reactor was inoculated with granular sludge obtained from a pulp and paper industry and fed at a constant inlet sulfate concentration of 250 mg S-SO42-L-1 and a constant C/S ratio of 1.5 ± 0.3 g Cg-1 S for over 500 days. Apart from the regular analysis of chemical species, Illumina analyses of the 16S rRNA gene were used to study the dynamics of the bacterial community along with the whole operation. The reactor was sampled along the operation to monitor its diversity and the changes in targeted species to gain insight into the performance of the sulfidogenic UASB. Moreover, studies on the stratification of the sludge bed were performed by sampling at different reactor heights. Shifts in the UASB performance correlated well with the main shifts in microbial communities of interest. A progressive loss of the methanogenic capacity towards a fully sulfidogenic UASB was explained by a progressive wash-out of methanogenic Archaea, which were outcompeted by sulfate-reducing bacteria. Desulfovibrio was found as the main sulfate-reducing genus in the reactor along time. A progressive reduction in the sulfidogenic capacity of the UASB was found in the long run due to the accumulation of a slime-like substance in the UASB. 22021-01-0120212021-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/236673https://dx.doi.org/urn:doi:10.3390/ijerph18031305reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 RTI2018-099362-B-C21open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2366732026-06-06T12:50:31Z
dc.title.none.fl_str_mv Microbial diversity dynamics in a methanogenic-sulfidogenic UASB reactor
title Microbial diversity dynamics in a methanogenic-sulfidogenic UASB reactor
spellingShingle Microbial diversity dynamics in a methanogenic-sulfidogenic UASB reactor
Fernandez Palacios, Eva|||0000-0002-0095-220X
Bioscrubber
Crude glycerol
UASB
Sulfate-reducing bacteria
Microbial diversity shifts
Sequencing
title_short Microbial diversity dynamics in a methanogenic-sulfidogenic UASB reactor
title_full Microbial diversity dynamics in a methanogenic-sulfidogenic UASB reactor
title_fullStr Microbial diversity dynamics in a methanogenic-sulfidogenic UASB reactor
title_full_unstemmed Microbial diversity dynamics in a methanogenic-sulfidogenic UASB reactor
title_sort Microbial diversity dynamics in a methanogenic-sulfidogenic UASB reactor
dc.creator.none.fl_str_mv Fernandez Palacios, Eva|||0000-0002-0095-220X
Zhou, Xudong
Mora Garrido, Mabel|||0000-0002-2979-3601
Gabriel, David|||0000-0002-7713-4192
author Fernandez Palacios, Eva|||0000-0002-0095-220X
author_facet Fernandez Palacios, Eva|||0000-0002-0095-220X
Zhou, Xudong
Mora Garrido, Mabel|||0000-0002-2979-3601
Gabriel, David|||0000-0002-7713-4192
author_role author
author2 Zhou, Xudong
Mora Garrido, Mabel|||0000-0002-2979-3601
Gabriel, David|||0000-0002-7713-4192
author2_role author
author
author
dc.subject.none.fl_str_mv Bioscrubber
Crude glycerol
UASB
Sulfate-reducing bacteria
Microbial diversity shifts
Sequencing
topic Bioscrubber
Crude glycerol
UASB
Sulfate-reducing bacteria
Microbial diversity shifts
Sequencing
description In this study, the long-term performance and microbial dynamics of an Upflow Anaerobic Sludge Blanket (UASB) reactor targeting sulfate reduction in a SOx emissions treatment system were assessed using crude glycerol as organic carbon source and electron donor under constant S and C loading rates. The reactor was inoculated with granular sludge obtained from a pulp and paper industry and fed at a constant inlet sulfate concentration of 250 mg S-SO42-L-1 and a constant C/S ratio of 1.5 ± 0.3 g Cg-1 S for over 500 days. Apart from the regular analysis of chemical species, Illumina analyses of the 16S rRNA gene were used to study the dynamics of the bacterial community along with the whole operation. The reactor was sampled along the operation to monitor its diversity and the changes in targeted species to gain insight into the performance of the sulfidogenic UASB. Moreover, studies on the stratification of the sludge bed were performed by sampling at different reactor heights. Shifts in the UASB performance correlated well with the main shifts in microbial communities of interest. A progressive loss of the methanogenic capacity towards a fully sulfidogenic UASB was explained by a progressive wash-out of methanogenic Archaea, which were outcompeted by sulfate-reducing bacteria. Desulfovibrio was found as the main sulfate-reducing genus in the reactor along time. A progressive reduction in the sulfidogenic capacity of the UASB was found in the long run due to the accumulation of a slime-like substance in the UASB.
publishDate 2021
dc.date.none.fl_str_mv 2
2021-01-01
2021
2021-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/236673
https://dx.doi.org/urn:doi:10.3390/ijerph18031305
url https://ddd.uab.cat/record/236673
https://dx.doi.org/urn:doi:10.3390/ijerph18031305
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 RTI2018-099362-B-C21
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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