Three antifungal proteins from penicillium expansum

Antifungal proteins of fungal origin (AFPs) are small, secreted, cationic, and cysteine-rich proteins. Filamentous fungi encode a wide repertoire of AFPs belonging to different phylogenetic classes, which offer a great potential to develop new antifungals for the control of pathogenic fungi. The fun...

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Autores: Garrigues, Sandra, Gandía, Mónica, Castillo, Laia, Coca López, María|||0000-0002-5578-3175, Marx, Florentine, Marcos, José F., Manzanares, Paloma
Formato: artículo
Fecha de publicación:2018
País:España
Recursos:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:218116
Acesso em linha:https://ddd.uab.cat/record/218116
https://dx.doi.org/urn:doi:10.3389/fmicb.2018.02370
Access Level:acceso abierto
Palavra-chave:Penicillium expansum
PeAfpA
Penicillium chrysogenum
Penicillium digitatum
Botrytis cinerea
Postharvest
Crop protection
Pathogenic fungi
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spelling Three antifungal proteins from penicillium expansumdifferent patterns of production and antifungal activityGarrigues, SandraGandía, MónicaCastillo, LaiaCoca López, María|||0000-0002-5578-3175Marx, FlorentineMarcos, José F.Manzanares, PalomaPenicillium expansumPeAfpAPenicillium chrysogenumPenicillium digitatumBotrytis cinereaPostharvestCrop protectionPathogenic fungiAntifungal proteins of fungal origin (AFPs) are small, secreted, cationic, and cysteine-rich proteins. Filamentous fungi encode a wide repertoire of AFPs belonging to different phylogenetic classes, which offer a great potential to develop new antifungals for the control of pathogenic fungi. The fungus Penicillium expansum is one of the few reported to encode three AFPs each belonging to a different phylogenetic class (A, B, and C). In this work, the production of the putative AFPs from P. expansum was evaluated, but only the representative of class A, PeAfpA, was identified in culture supernatants of the native fungus. The biotechnological production of PeAfpB and PeAfpC was achieved in Penicillium chrysogenum with the P. chrysogenum-based expression cassette, which had been proved to work efficiently for the production of other related AFPs in filamentous fungi. Western blot analyses confirmed that P. expansum only produces PeAfpA naturally, whereas PeAfpB and PeAfpC could not be detected. From the three AFPs from P. expansum, PeAfpA showed the highest antifungal activity against all fungi tested, including plant and human pathogens. P. expansum was also sensitive to its self-AFPs PeAfpA and PeAfpB. PeAfpB showed moderate antifungal activity against filamentous fungi, whereas no activity could be attributed to PeAfpC at the conditions tested. Importantly, none of the PeAFPs showed hemolytic activity. Finally, PeAfpA was demonstrated to efficiently protect against fungal infections caused by Botrytis cinerea in tomato leaves and Penicillium digitatum in oranges. The strong antifungal potency of PeAfpA, together with the lack of cytotoxicity, and significant in vivo protection against phytopathogenic fungi that cause postharvest decay and plant diseases, make PeAfpA a promising alternative compound for application in agriculture, but also in medicine or food preservation. 22018-01-0120182018-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/218116https://dx.doi.org/urn:doi:10.3389/fmicb.2018.02370reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 BIO2015-68790-C2-2-RMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 BIO2015-68790-C2-1-RMinisterio de Educación, Cultura y Deporte https://doi.org/10.13039/501100003176 FPU13/04584open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2181162026-06-06T12:50:31Z
dc.title.none.fl_str_mv Three antifungal proteins from penicillium expansum
different patterns of production and antifungal activity
title Three antifungal proteins from penicillium expansum
spellingShingle Three antifungal proteins from penicillium expansum
Garrigues, Sandra
Penicillium expansum
PeAfpA
Penicillium chrysogenum
Penicillium digitatum
Botrytis cinerea
Postharvest
Crop protection
Pathogenic fungi
title_short Three antifungal proteins from penicillium expansum
title_full Three antifungal proteins from penicillium expansum
title_fullStr Three antifungal proteins from penicillium expansum
title_full_unstemmed Three antifungal proteins from penicillium expansum
title_sort Three antifungal proteins from penicillium expansum
dc.creator.none.fl_str_mv Garrigues, Sandra
Gandía, Mónica
Castillo, Laia
Coca López, María|||0000-0002-5578-3175
Marx, Florentine
Marcos, José F.
Manzanares, Paloma
author Garrigues, Sandra
author_facet Garrigues, Sandra
Gandía, Mónica
Castillo, Laia
Coca López, María|||0000-0002-5578-3175
Marx, Florentine
Marcos, José F.
Manzanares, Paloma
author_role author
author2 Gandía, Mónica
Castillo, Laia
Coca López, María|||0000-0002-5578-3175
Marx, Florentine
Marcos, José F.
Manzanares, Paloma
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Penicillium expansum
PeAfpA
Penicillium chrysogenum
Penicillium digitatum
Botrytis cinerea
Postharvest
Crop protection
Pathogenic fungi
topic Penicillium expansum
PeAfpA
Penicillium chrysogenum
Penicillium digitatum
Botrytis cinerea
Postharvest
Crop protection
Pathogenic fungi
description Antifungal proteins of fungal origin (AFPs) are small, secreted, cationic, and cysteine-rich proteins. Filamentous fungi encode a wide repertoire of AFPs belonging to different phylogenetic classes, which offer a great potential to develop new antifungals for the control of pathogenic fungi. The fungus Penicillium expansum is one of the few reported to encode three AFPs each belonging to a different phylogenetic class (A, B, and C). In this work, the production of the putative AFPs from P. expansum was evaluated, but only the representative of class A, PeAfpA, was identified in culture supernatants of the native fungus. The biotechnological production of PeAfpB and PeAfpC was achieved in Penicillium chrysogenum with the P. chrysogenum-based expression cassette, which had been proved to work efficiently for the production of other related AFPs in filamentous fungi. Western blot analyses confirmed that P. expansum only produces PeAfpA naturally, whereas PeAfpB and PeAfpC could not be detected. From the three AFPs from P. expansum, PeAfpA showed the highest antifungal activity against all fungi tested, including plant and human pathogens. P. expansum was also sensitive to its self-AFPs PeAfpA and PeAfpB. PeAfpB showed moderate antifungal activity against filamentous fungi, whereas no activity could be attributed to PeAfpC at the conditions tested. Importantly, none of the PeAFPs showed hemolytic activity. Finally, PeAfpA was demonstrated to efficiently protect against fungal infections caused by Botrytis cinerea in tomato leaves and Penicillium digitatum in oranges. The strong antifungal potency of PeAfpA, together with the lack of cytotoxicity, and significant in vivo protection against phytopathogenic fungi that cause postharvest decay and plant diseases, make PeAfpA a promising alternative compound for application in agriculture, but also in medicine or food preservation.
publishDate 2018
dc.date.none.fl_str_mv 2
2018-01-01
2018
2018-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/218116
https://dx.doi.org/urn:doi:10.3389/fmicb.2018.02370
url https://ddd.uab.cat/record/218116
https://dx.doi.org/urn:doi:10.3389/fmicb.2018.02370
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 BIO2015-68790-C2-2-R
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 BIO2015-68790-C2-1-R
Ministerio de Educación, Cultura y Deporte https://doi.org/10.13039/501100003176 FPU13/04584
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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