FoxO inhibits juvenile hormone biosynthesis and vitellogenin production in the German cockroach

The transcription factor Forkhead-box O (FoxO) is the main transcriptional effector of the Insulin Receptor/Phosphatidylinositol 3-kinase (InR/PI3K) pathway. In a situation of nutrient restriction, the pathway is inactive and FoxO translocates to the nucleus to exert its transcriptional action. In s...

Descripción completa

Detalles Bibliográficos
Autores: Castillo, Songül Süren, Abrisqueta, Marc, Maestro, José L.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2012
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/112627
Acceso en línea:http://hdl.handle.net/10261/112627
Access Level:acceso abierto
Palabra clave:FoxO
Nutritional signalling
Blattella germanica
Vitellogenin
Juvenile hormone
Insulin
id ES_3e050f1d4f73fc25468a9b0ee3d5dc9d
oai_identifier_str oai:digital.csic.es:10261/112627
network_acronym_str ES
network_name_str España
repository_id_str
spelling FoxO inhibits juvenile hormone biosynthesis and vitellogenin production in the German cockroachCastillo, Songül SürenAbrisqueta, MarcMaestro, José L.FoxONutritional signallingBlattella germanicaVitellogeninJuvenile hormoneInsulinThe transcription factor Forkhead-box O (FoxO) is the main transcriptional effector of the Insulin Receptor/Phosphatidylinositol 3-kinase (InR/PI3K) pathway. In a situation of nutrient restriction, the pathway is inactive and FoxO translocates to the nucleus to exert its transcriptional action. In starved females of the cockroach . Blattella germanica, the reproductive processes, and in particular the synthesis of juvenile hormone in the corpora allata and that of vitellogenin in the fat body, are arrested. In the present report we examine the possible role of FoxO in the transduction of the nutritional signals to these reproductive events. We first cloned FoxO cDNA from . B. germanica (BgFoxO), and showed that its expression is not nutritionally regulated. BgFoxO knockdown using systemic RNAi . in vivo in starved females elicited an increase of juvenile hormone biosynthesis, although without modifying mRNA levels of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase-1, HMG-CoA synthase-2, HMG-CoA reductase or methyl farnesoate epoxidase (CYP15A1) in corpora allata. In addition, BgFoxO RNAi treatment produced a remarkable increase of vitellogenin mRNA levels in fat body and of vitellogenin protein in the haemolymph. Our results indicate that BgFoxO plays an inhibitory role on juvenile hormone biosynthesis and vitellogenin production in a situation of nutrient shortage. © 2012 Elsevier Ltd.This work was supported by grants BFU2006-01090/BFI (Spanish Ministry of Science and Innovation (MICINN) and FEDER) and BFU2010-15906 (MICINN) to J.L.M. M.A. and S. S.-C. are recipients of a pre-doctoral fellowship (MICINN) and a post-doctoral contract (CSIC, JAE program co-funded by the European Social Fund), respectively.Peer ReviewedElsevierMinisterio de Ciencia e Innovación (España)European CommissionConsejo Superior de Investigaciones Científicas (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2015201520122015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/112627reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.ibmb.2012.03.006Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1126272026-05-22T06:33:51Z
dc.title.none.fl_str_mv FoxO inhibits juvenile hormone biosynthesis and vitellogenin production in the German cockroach
title FoxO inhibits juvenile hormone biosynthesis and vitellogenin production in the German cockroach
spellingShingle FoxO inhibits juvenile hormone biosynthesis and vitellogenin production in the German cockroach
Castillo, Songül Süren
FoxO
Nutritional signalling
Blattella germanica
Vitellogenin
Juvenile hormone
Insulin
title_short FoxO inhibits juvenile hormone biosynthesis and vitellogenin production in the German cockroach
title_full FoxO inhibits juvenile hormone biosynthesis and vitellogenin production in the German cockroach
title_fullStr FoxO inhibits juvenile hormone biosynthesis and vitellogenin production in the German cockroach
title_full_unstemmed FoxO inhibits juvenile hormone biosynthesis and vitellogenin production in the German cockroach
title_sort FoxO inhibits juvenile hormone biosynthesis and vitellogenin production in the German cockroach
dc.creator.none.fl_str_mv Castillo, Songül Süren
Abrisqueta, Marc
Maestro, José L.
author Castillo, Songül Süren
author_facet Castillo, Songül Süren
Abrisqueta, Marc
Maestro, José L.
author_role author
author2 Abrisqueta, Marc
Maestro, José L.
author2_role author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
European Commission
Consejo Superior de Investigaciones Científicas (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv FoxO
Nutritional signalling
Blattella germanica
Vitellogenin
Juvenile hormone
Insulin
topic FoxO
Nutritional signalling
Blattella germanica
Vitellogenin
Juvenile hormone
Insulin
description The transcription factor Forkhead-box O (FoxO) is the main transcriptional effector of the Insulin Receptor/Phosphatidylinositol 3-kinase (InR/PI3K) pathway. In a situation of nutrient restriction, the pathway is inactive and FoxO translocates to the nucleus to exert its transcriptional action. In starved females of the cockroach . Blattella germanica, the reproductive processes, and in particular the synthesis of juvenile hormone in the corpora allata and that of vitellogenin in the fat body, are arrested. In the present report we examine the possible role of FoxO in the transduction of the nutritional signals to these reproductive events. We first cloned FoxO cDNA from . B. germanica (BgFoxO), and showed that its expression is not nutritionally regulated. BgFoxO knockdown using systemic RNAi . in vivo in starved females elicited an increase of juvenile hormone biosynthesis, although without modifying mRNA levels of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase-1, HMG-CoA synthase-2, HMG-CoA reductase or methyl farnesoate epoxidase (CYP15A1) in corpora allata. In addition, BgFoxO RNAi treatment produced a remarkable increase of vitellogenin mRNA levels in fat body and of vitellogenin protein in the haemolymph. Our results indicate that BgFoxO plays an inhibitory role on juvenile hormone biosynthesis and vitellogenin production in a situation of nutrient shortage. © 2012 Elsevier Ltd.
publishDate 2012
dc.date.none.fl_str_mv 2012
2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/112627
url http://hdl.handle.net/10261/112627
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.ibmb.2012.03.006
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869406491900903424
score 15,812429

Similares en LA Referencia