Extracellular Vesicles Derived from Plasmodium-infected and Non-infected Red Blood Cells as Targeted Drug Delivery Vehicles

Among several factors behind drug resistance evolution in malaria is the challenge of administering overall doses that are not toxic for the patient but that, locally, are sufficiently high to rapidly kill the parasites. Thus, a crucial antimalarial strategy is the development of drug delivery syste...

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Authors: Borgheti Cardoso, Livia Neves, Kooijmans, Sander A. A., Gutiérrez Chamorro, Lucía, Biosca, Arnau, Lantero, Elena, Ramírez, Miriam, Avalos Padilla, Yunuen, Crespo, Isabel, Fernandez Vidal, Irene, Fernández Becerra, Carmen, Portillo Obando, Hernando A. del, Fernàndez Busquets, Xavier
Format: article
Status:Versión aceptada para publicación
Publication Date:2020
Country:España
Institution:Universidad de Barcelona
Repository:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/182977
Online Access:https://hdl.handle.net/2445/182977
Access Level:Open access
Keyword:Malària
Hematies
Malaria
Erythrocytes
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spelling Extracellular Vesicles Derived from Plasmodium-infected and Non-infected Red Blood Cells as Targeted Drug Delivery VehiclesBorgheti Cardoso, Livia NevesKooijmans, Sander A. A.Gutiérrez Chamorro, LucíaBiosca, ArnauLantero, ElenaRamírez, MiriamAvalos Padilla, YunuenCrespo, IsabelFernandez Vidal, IreneFernández Becerra, CarmenPortillo Obando, Hernando A. delFernàndez Busquets, XavierMalàriaHematiesMalariaErythrocytesAmong several factors behind drug resistance evolution in malaria is the challenge of administering overall doses that are not toxic for the patient but that, locally, are sufficiently high to rapidly kill the parasites. Thus, a crucial antimalarial strategy is the development of drug delivery systems capable of targeting antimalarial compounds to Plasmodium with high specificity. In the present study, extracellular vesicles (EVs) have been evaluated as a drug delivery system for the treatment of malaria. EVs derived from naive red blood cells (RBCs) and from Plasmodium falciparum-infected RBCs (pRBCs) were isolated by ultrafiltration followed by size exclusion chromatography. Lipidomic characterization showed that there were no significant qualitative differences between the lipidomic profiles of pRBC-derived EVs (pRBC-EVs) and RBC-derived EVs (RBC-EVs). Both EVs were taken up by RBCs and pRBCs, although pRBC-EVs were more efficiently internalized than RBC-EVs, which suggested their potential use as drug delivery vehicles for these cells. When loaded into pRBC-EVs, the antimalarial drugs atovaquone and tafenoquine inhibited in vitro P. falciparum growth more efficiently than their free drug counterparts, indicating that pRBC-EVs can potentially increase the efficacy of several small hydrophobic drugs used for the treatment of malaria.Elsevier2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/182977Articles publicats en revistes (ISGlobal)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: http://dx.doi.org/ 10.1016/j.ijpharm.2020.119627International Journal of Pharmaceutics, 2020, vol 587, num.119627http://dx.doi.org/ 10.1016/j.ijpharm.2020.119627(c) Elsevier, 2020info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1829772026-05-27T06:46:51Z
dc.title.none.fl_str_mv Extracellular Vesicles Derived from Plasmodium-infected and Non-infected Red Blood Cells as Targeted Drug Delivery Vehicles
title Extracellular Vesicles Derived from Plasmodium-infected and Non-infected Red Blood Cells as Targeted Drug Delivery Vehicles
spellingShingle Extracellular Vesicles Derived from Plasmodium-infected and Non-infected Red Blood Cells as Targeted Drug Delivery Vehicles
Borgheti Cardoso, Livia Neves
Malària
Hematies
Malaria
Erythrocytes
title_short Extracellular Vesicles Derived from Plasmodium-infected and Non-infected Red Blood Cells as Targeted Drug Delivery Vehicles
title_full Extracellular Vesicles Derived from Plasmodium-infected and Non-infected Red Blood Cells as Targeted Drug Delivery Vehicles
title_fullStr Extracellular Vesicles Derived from Plasmodium-infected and Non-infected Red Blood Cells as Targeted Drug Delivery Vehicles
title_full_unstemmed Extracellular Vesicles Derived from Plasmodium-infected and Non-infected Red Blood Cells as Targeted Drug Delivery Vehicles
title_sort Extracellular Vesicles Derived from Plasmodium-infected and Non-infected Red Blood Cells as Targeted Drug Delivery Vehicles
dc.creator.none.fl_str_mv Borgheti Cardoso, Livia Neves
Kooijmans, Sander A. A.
Gutiérrez Chamorro, Lucía
Biosca, Arnau
Lantero, Elena
Ramírez, Miriam
Avalos Padilla, Yunuen
Crespo, Isabel
Fernandez Vidal, Irene
Fernández Becerra, Carmen
Portillo Obando, Hernando A. del
Fernàndez Busquets, Xavier
author Borgheti Cardoso, Livia Neves
author_facet Borgheti Cardoso, Livia Neves
Kooijmans, Sander A. A.
Gutiérrez Chamorro, Lucía
Biosca, Arnau
Lantero, Elena
Ramírez, Miriam
Avalos Padilla, Yunuen
Crespo, Isabel
Fernandez Vidal, Irene
Fernández Becerra, Carmen
Portillo Obando, Hernando A. del
Fernàndez Busquets, Xavier
author_role author
author2 Kooijmans, Sander A. A.
Gutiérrez Chamorro, Lucía
Biosca, Arnau
Lantero, Elena
Ramírez, Miriam
Avalos Padilla, Yunuen
Crespo, Isabel
Fernandez Vidal, Irene
Fernández Becerra, Carmen
Portillo Obando, Hernando A. del
Fernàndez Busquets, Xavier
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Malària
Hematies
Malaria
Erythrocytes
topic Malària
Hematies
Malaria
Erythrocytes
description Among several factors behind drug resistance evolution in malaria is the challenge of administering overall doses that are not toxic for the patient but that, locally, are sufficiently high to rapidly kill the parasites. Thus, a crucial antimalarial strategy is the development of drug delivery systems capable of targeting antimalarial compounds to Plasmodium with high specificity. In the present study, extracellular vesicles (EVs) have been evaluated as a drug delivery system for the treatment of malaria. EVs derived from naive red blood cells (RBCs) and from Plasmodium falciparum-infected RBCs (pRBCs) were isolated by ultrafiltration followed by size exclusion chromatography. Lipidomic characterization showed that there were no significant qualitative differences between the lipidomic profiles of pRBC-derived EVs (pRBC-EVs) and RBC-derived EVs (RBC-EVs). Both EVs were taken up by RBCs and pRBCs, although pRBC-EVs were more efficiently internalized than RBC-EVs, which suggested their potential use as drug delivery vehicles for these cells. When loaded into pRBC-EVs, the antimalarial drugs atovaquone and tafenoquine inhibited in vitro P. falciparum growth more efficiently than their free drug counterparts, indicating that pRBC-EVs can potentially increase the efficacy of several small hydrophobic drugs used for the treatment of malaria.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/182977
url https://hdl.handle.net/2445/182977
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: http://dx.doi.org/ 10.1016/j.ijpharm.2020.119627
International Journal of Pharmaceutics, 2020, vol 587, num.119627
http://dx.doi.org/ 10.1016/j.ijpharm.2020.119627
dc.rights.none.fl_str_mv (c) Elsevier, 2020
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Elsevier, 2020
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Articles publicats en revistes (ISGlobal)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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