Highly informative SSR genotyping reveals large genetic diversity and limited differentiation in European larch (Larix decidua) populations from Romania
[EN] European larch (Larix decidua) is naturally distributed at high elevations in Central Europe. Romanian populations of L. decidua are scattered in different areas of the country. In this study, we used 12 highly informative genomic simple sequence repeat (SSR) markers for genotyping seven popula...
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:riunet.upv.es:10251/120244 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/120244 |
| Access Level: | acceso abierto |
| Palabra clave: | Analysis of molecular variance Heterozygosity Genetic diversity Larix SSR markers BIOQUIMICA Y BIOLOGIA MOLECULAR BOTANICA GENETICA |
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Highly informative SSR genotyping reveals large genetic diversity and limited differentiation in European larch (Larix decidua) populations from RomaniaGramazio, Pietro|||0000-0003-2226-7999Vilanova Navarro, Santiago|||0000-0003-4939-9713Plazas Ávila, María de la O|||0000-0001-8090-7312Vicente, Oscar|||0000-0001-5076-3784Boscaiu, Monica|||0000-0002-9691-4223Prohens Tomás, Jaime|||0000-0003-1181-9065Plesa, Iona M.Truta, Alina M.Sestras, Adriana F.Sestras, Radu E.Analysis of molecular varianceHeterozygosityGenetic diversityLarixSSR markersBIOQUIMICA Y BIOLOGIA MOLECULARBOTANICAGENETICA[EN] European larch (Larix decidua) is naturally distributed at high elevations in Central Europe. Romanian populations of L. decidua are scattered in different areas of the country. In this study, we used 12 highly informative genomic simple sequence repeat (SSR) markers for genotyping seven populations from different areas of Romania. The SSR markers were highly variable, with up to 11 alleles per SSR locus and an average polymorphic information content of 0.713. High values of observed (Ho = 0.542) and expected (He = 0.738) heterozygosities were observed. Cluster analysis of populations did not group them according to geographical distance, but some clusters contained populations from a similar altitudinal range. The partition of genetic variation revealed that two-thirds of the genetic variation was found within individuals (due to high Ho levels), while only one-sixth of the total genetic variation corresponded to variation among populations. A population structure analysis identified four genetic clusters, and in most cases individuals from a single population were assigned to several genetic clusters. Multivariate principal coordinates analysis confirmed the population structure analysis. SSR markers are a powerful tool for evaluating diversity, relationships, and the genetic structure of Romanian L. decidua populations, which have high levels of diversity and low genetic structurationPietro Gramazio is grateful to Universitat Politecnica de Valencia for a predoctoral contract (Programa FPI de la UPV-Subprograma 1/2013 call). Ioana M Plesa is grateful to the Doctoral School of the University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca and ICHAT - Institute of Advanced Horticulture Research of Transylvania, and Universitat Politecnica de Valencia, for their support. Mariola Plazas is grateful to Spanish Ministerio de Economia, Industria y Competitividad for a post doctoral grant within the Juan de la Cierva programme (FCJI-2015-24835).Scientific and Technological Research Council of TurkeyDepartamento de Ecosistemas AgroforestalesDepartamento de BiotecnologíaInstituto Universitario de Conservación y Mejora de la Agrodiversidad ValencianaInstituto Agroforestal MediterráneoEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalMinisterio de Economía y CompetitividadRepositorio Institucional de la Universitat Politècnica de València Riunet20182018-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/120244reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 FJCI-2015-24835 FJCI-2015-24835open accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1202442026-06-13T07:49:27Z |
| dc.title.none.fl_str_mv |
Highly informative SSR genotyping reveals large genetic diversity and limited differentiation in European larch (Larix decidua) populations from Romania |
| title |
Highly informative SSR genotyping reveals large genetic diversity and limited differentiation in European larch (Larix decidua) populations from Romania |
| spellingShingle |
Highly informative SSR genotyping reveals large genetic diversity and limited differentiation in European larch (Larix decidua) populations from Romania Gramazio, Pietro|||0000-0003-2226-7999 Analysis of molecular variance Heterozygosity Genetic diversity Larix SSR markers BIOQUIMICA Y BIOLOGIA MOLECULAR BOTANICA GENETICA |
| title_short |
Highly informative SSR genotyping reveals large genetic diversity and limited differentiation in European larch (Larix decidua) populations from Romania |
| title_full |
Highly informative SSR genotyping reveals large genetic diversity and limited differentiation in European larch (Larix decidua) populations from Romania |
| title_fullStr |
Highly informative SSR genotyping reveals large genetic diversity and limited differentiation in European larch (Larix decidua) populations from Romania |
| title_full_unstemmed |
Highly informative SSR genotyping reveals large genetic diversity and limited differentiation in European larch (Larix decidua) populations from Romania |
| title_sort |
Highly informative SSR genotyping reveals large genetic diversity and limited differentiation in European larch (Larix decidua) populations from Romania |
| dc.creator.none.fl_str_mv |
Gramazio, Pietro|||0000-0003-2226-7999 Vilanova Navarro, Santiago|||0000-0003-4939-9713 Plazas Ávila, María de la O|||0000-0001-8090-7312 Vicente, Oscar|||0000-0001-5076-3784 Boscaiu, Monica|||0000-0002-9691-4223 Prohens Tomás, Jaime|||0000-0003-1181-9065 Plesa, Iona M. Truta, Alina M. Sestras, Adriana F. Sestras, Radu E. |
| author |
Gramazio, Pietro|||0000-0003-2226-7999 |
| author_facet |
Gramazio, Pietro|||0000-0003-2226-7999 Vilanova Navarro, Santiago|||0000-0003-4939-9713 Plazas Ávila, María de la O|||0000-0001-8090-7312 Vicente, Oscar|||0000-0001-5076-3784 Boscaiu, Monica|||0000-0002-9691-4223 Prohens Tomás, Jaime|||0000-0003-1181-9065 Plesa, Iona M. Truta, Alina M. Sestras, Adriana F. Sestras, Radu E. |
| author_role |
author |
| author2 |
Vilanova Navarro, Santiago|||0000-0003-4939-9713 Plazas Ávila, María de la O|||0000-0001-8090-7312 Vicente, Oscar|||0000-0001-5076-3784 Boscaiu, Monica|||0000-0002-9691-4223 Prohens Tomás, Jaime|||0000-0003-1181-9065 Plesa, Iona M. Truta, Alina M. Sestras, Adriana F. Sestras, Radu E. |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Departamento de Ecosistemas Agroforestales Departamento de Biotecnología Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana Instituto Agroforestal Mediterráneo Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural Ministerio de Economía y Competitividad Repositorio Institucional de la Universitat Politècnica de València Riunet |
| dc.subject.none.fl_str_mv |
Analysis of molecular variance Heterozygosity Genetic diversity Larix SSR markers BIOQUIMICA Y BIOLOGIA MOLECULAR BOTANICA GENETICA |
| topic |
Analysis of molecular variance Heterozygosity Genetic diversity Larix SSR markers BIOQUIMICA Y BIOLOGIA MOLECULAR BOTANICA GENETICA |
| description |
[EN] European larch (Larix decidua) is naturally distributed at high elevations in Central Europe. Romanian populations of L. decidua are scattered in different areas of the country. In this study, we used 12 highly informative genomic simple sequence repeat (SSR) markers for genotyping seven populations from different areas of Romania. The SSR markers were highly variable, with up to 11 alleles per SSR locus and an average polymorphic information content of 0.713. High values of observed (Ho = 0.542) and expected (He = 0.738) heterozygosities were observed. Cluster analysis of populations did not group them according to geographical distance, but some clusters contained populations from a similar altitudinal range. The partition of genetic variation revealed that two-thirds of the genetic variation was found within individuals (due to high Ho levels), while only one-sixth of the total genetic variation corresponded to variation among populations. A population structure analysis identified four genetic clusters, and in most cases individuals from a single population were assigned to several genetic clusters. Multivariate principal coordinates analysis confirmed the population structure analysis. SSR markers are a powerful tool for evaluating diversity, relationships, and the genetic structure of Romanian L. decidua populations, which have high levels of diversity and low genetic structuration |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018 2018-01-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://riunet.upv.es/handle/10251/120244 |
| url |
https://riunet.upv.es/handle/10251/120244 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 FJCI-2015-24835 FJCI-2015-24835 |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Reserva de todos los derechos http://rightsstatements.org/vocab/InC/1.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 Reserva de todos los derechos http://rightsstatements.org/vocab/InC/1.0/ |
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openAccess |
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application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
Scientific and Technological Research Council of Turkey |
| publisher.none.fl_str_mv |
Scientific and Technological Research Council of Turkey |
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reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname:Universitat Politècnica de València (UPV) |
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Universitat Politècnica de València (UPV) |
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RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
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RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
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