Serum-derived extracellular vesicles from african swine fever virus-infected pigs selectively recruit viral and porcine proteins

African swine fever is a devastating hemorrhagic infectious disease, which affects domestic and wild swines (Sus scrofa) of all breeds and ages, with a high lethality of up to 90–100% in naïve animals. The causative agent, African swine fever virus (ASFV), is a large and complex double-stranded DNA...

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Autores: Montaner-Tarbes, Sergio, Pujol, Myriam, Jabbar, Tamara, Hawes, Philippa, Chapman, Dave, Portillo, Hernando del, Fraile, Lorenzo, Sánchez-Cordón, Pedro J., Dixon, Linda, Montoya, María
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/193513
Acceso en línea:http://hdl.handle.net/10261/193513
Access Level:acceso abierto
Palabra clave:Extracellular vesicles
African swine fever virus
Proteomic analysis
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spelling Serum-derived extracellular vesicles from african swine fever virus-infected pigs selectively recruit viral and porcine proteinsMontaner-Tarbes, SergioPujol, MyriamJabbar, TamaraHawes, PhilippaChapman, DavePortillo, Hernando delFraile, LorenzoSánchez-Cordón, Pedro J.Dixon, LindaMontoya, MaríaExtracellular vesiclesAfrican swine fever virusProteomic analysisAfrican swine fever is a devastating hemorrhagic infectious disease, which affects domestic and wild swines (Sus scrofa) of all breeds and ages, with a high lethality of up to 90–100% in naïve animals. The causative agent, African swine fever virus (ASFV), is a large and complex double-stranded DNA arbovirus which is currently spreading worldwide, with serious socioeconomic consequences. There is no treatment or effective vaccine commercially available, and most of the current research is focused on attenuated viral models, with limited success so far. Thus, new strategies are under investigation. Extracellular vesicles (EVs) have proven to be a promising new vaccination platform for veterinary diseases in situations in which conventional approaches have not been completely successful. Here, serum extracellular vesicles from infected pigs using two different ASFV viruses (OURT 88/3 and Benin ΔMGF), corresponding to a naturally attenuated virus and a deletion mutant, respectively, were characterized in order to determine possible differences in the content of swine and viral proteins in EV-enriched fractions. Firstly, EVs were characterized by their CD5, CD63, CD81 and CD163 surface expression. Secondly, ASFV proteins were detected on the surface of EVs from ASFV-infected pig serum. Finally, proteomic analysis revealed few specific proteins from ASFV in the EVs, but 942 swine proteins were detected in all EV preparations (negative controls, and OURT 88/3 and Benin ΔMGF-infected preparations). However, in samples from OURT 88/3-infected animals, only a small number of proteins were differentially identified compared to control uninfected animals. Fifty-six swine proteins (Group Benin) and seven proteins (Group OURT 88/3) were differentially detected on EVs when compared to the EV control group. Most of these were related to coagulation cascades. The results presented here could contribute to a better understanding of ASFV pathogenesis and immune/protective responses in the host.Sergio Montaner Tarbes was funded by an Industrial PhD Fellowship by Doctorats Industrials de Catalonia (Agència de Gestió d’Ajuts Universitaris i de Recerca). This article is based upon work from COST Action CA15116 ASF-STOP, supported by COST (European Cooperation in Science and Technology) and the UK’s BBSRC grants BBS/E/I/00002014 and BB/L004267/1.Peer reviewedMultidisciplinary Digital Publishing InstituteGeneralitat de CatalunyaEuropean Cooperation in Science and TechnologyBiotechnology and Biological Sciences Research Council (UK)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/193513reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3390/v11100882Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1935132026-05-22T06:33:51Z
dc.title.none.fl_str_mv Serum-derived extracellular vesicles from african swine fever virus-infected pigs selectively recruit viral and porcine proteins
title Serum-derived extracellular vesicles from african swine fever virus-infected pigs selectively recruit viral and porcine proteins
spellingShingle Serum-derived extracellular vesicles from african swine fever virus-infected pigs selectively recruit viral and porcine proteins
Montaner-Tarbes, Sergio
Extracellular vesicles
African swine fever virus
Proteomic analysis
title_short Serum-derived extracellular vesicles from african swine fever virus-infected pigs selectively recruit viral and porcine proteins
title_full Serum-derived extracellular vesicles from african swine fever virus-infected pigs selectively recruit viral and porcine proteins
title_fullStr Serum-derived extracellular vesicles from african swine fever virus-infected pigs selectively recruit viral and porcine proteins
title_full_unstemmed Serum-derived extracellular vesicles from african swine fever virus-infected pigs selectively recruit viral and porcine proteins
title_sort Serum-derived extracellular vesicles from african swine fever virus-infected pigs selectively recruit viral and porcine proteins
dc.creator.none.fl_str_mv Montaner-Tarbes, Sergio
Pujol, Myriam
Jabbar, Tamara
Hawes, Philippa
Chapman, Dave
Portillo, Hernando del
Fraile, Lorenzo
Sánchez-Cordón, Pedro J.
Dixon, Linda
Montoya, María
author Montaner-Tarbes, Sergio
author_facet Montaner-Tarbes, Sergio
Pujol, Myriam
Jabbar, Tamara
Hawes, Philippa
Chapman, Dave
Portillo, Hernando del
Fraile, Lorenzo
Sánchez-Cordón, Pedro J.
Dixon, Linda
Montoya, María
author_role author
author2 Pujol, Myriam
Jabbar, Tamara
Hawes, Philippa
Chapman, Dave
Portillo, Hernando del
Fraile, Lorenzo
Sánchez-Cordón, Pedro J.
Dixon, Linda
Montoya, María
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Generalitat de Catalunya
European Cooperation in Science and Technology
Biotechnology and Biological Sciences Research Council (UK)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Extracellular vesicles
African swine fever virus
Proteomic analysis
topic Extracellular vesicles
African swine fever virus
Proteomic analysis
description African swine fever is a devastating hemorrhagic infectious disease, which affects domestic and wild swines (Sus scrofa) of all breeds and ages, with a high lethality of up to 90–100% in naïve animals. The causative agent, African swine fever virus (ASFV), is a large and complex double-stranded DNA arbovirus which is currently spreading worldwide, with serious socioeconomic consequences. There is no treatment or effective vaccine commercially available, and most of the current research is focused on attenuated viral models, with limited success so far. Thus, new strategies are under investigation. Extracellular vesicles (EVs) have proven to be a promising new vaccination platform for veterinary diseases in situations in which conventional approaches have not been completely successful. Here, serum extracellular vesicles from infected pigs using two different ASFV viruses (OURT 88/3 and Benin ΔMGF), corresponding to a naturally attenuated virus and a deletion mutant, respectively, were characterized in order to determine possible differences in the content of swine and viral proteins in EV-enriched fractions. Firstly, EVs were characterized by their CD5, CD63, CD81 and CD163 surface expression. Secondly, ASFV proteins were detected on the surface of EVs from ASFV-infected pig serum. Finally, proteomic analysis revealed few specific proteins from ASFV in the EVs, but 942 swine proteins were detected in all EV preparations (negative controls, and OURT 88/3 and Benin ΔMGF-infected preparations). However, in samples from OURT 88/3-infected animals, only a small number of proteins were differentially identified compared to control uninfected animals. Fifty-six swine proteins (Group Benin) and seven proteins (Group OURT 88/3) were differentially detected on EVs when compared to the EV control group. Most of these were related to coagulation cascades. The results presented here could contribute to a better understanding of ASFV pathogenesis and immune/protective responses in the host.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/193513
url http://hdl.handle.net/10261/193513
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3390/v11100882
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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