A polymorphism in the fatty acid desaturase-2 gene is associated with the arachidonic acid metabolism in pigs

Arachidonic acid (C20:4) is related to a wide range of biological effects including lipid homeostasis. The fatty acid desaturase-2 (FADS2) gene encodes for the delta-6-desaturase, which is involved in the biosynthesis of C20:4 from linoleic acid (C18:2). The purpose of this study was to characterise...

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Autores: Gol, Sofia, Pena i Subirà, Ramona Natacha, Rothschild, Max Frederick, Tor i Naudí, Marc, Estany Illa, Joan
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/65209
Acceso en línea:https://doi.org/10.1038/s41598-018-32710-w
http://hdl.handle.net/10459.1/65209
Access Level:acceso abierto
Palabra clave:Àcids grassos
Porcs
Animals--Cria i desenvolupament
Porcs--Genètica
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spelling A polymorphism in the fatty acid desaturase-2 gene is associated with the arachidonic acid metabolism in pigsGol, SofiaPena i Subirà, Ramona NatachaRothschild, Max FrederickTor i Naudí, MarcEstany Illa, JoanÀcids grassosPorcsAnimals--Cria i desenvolupamentPorcs--GenèticaArachidonic acid (C20:4) is related to a wide range of biological effects including lipid homeostasis. The fatty acid desaturase-2 (FADS2) gene encodes for the delta-6-desaturase, which is involved in the biosynthesis of C20:4 from linoleic acid (C18:2). The purpose of this study was to characterise mutations in the promoter of the porcine FADS2, evaluating in particular the effect of one haplotype tagging polymorphism (rs321384923A > G) on the biosynthesis pathway of C20:4. A total of 1,192 Duroc barrows with records on fatty acid composition in muscle and subcutaneous fat were genotyped. Pigs carrying the A allele showed, irrespective of fat content, both enhanced FADS2 expression and higher C20:4 in muscle and exhibited increased ratios of C20:4 to C18:2 and of C20:4 to eicosadienoic acid (C20:2) in both muscle and adipose tissue. Despite the inverse relationship observed between C20:4 and fat content, the rs321384923 polymorphism had no impact on lean weight. It is concluded that the haplotype encompassing the rs321384923 polymorphism at the porcine FADS2 affects the n-6 fatty acid profile by specifically modifying the desaturation efficiency of C18:2 to C20:4 rather than by concomitant variations in C18:2 following changes in fat content.This research has received funding from the Spanish Ministry of Economy and Competitiveness and the European Union Regional Development Funds (AGL2015–65846-R grant) and was partially supported by the Centre for the Development of Industrial Technology (IDI-20150115 project). SG is recipient of a PhD scholarship from the Spanish Ministry of Science and Innovation (BES-2014-FPU13/04975). We acknowledge the staff at Selección Batallé for their cooperation.Nature Publishing Group2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.1038/s41598-018-32710-whttp://hdl.handle.net/10459.1/65209reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)Inglésinfo:eu-repo/grantAgreement/MINECO//AGL2015-65846-Rinfo:eu-repo/grantAgreement/MINECO//BES-2014-FPU13%2F04975Reproducció del document publicat a: https://doi.org/10.1038/s41598-018-32710-wScientific Reports, 2018, p. vol. 8, núm. 14336, p.1-9cc-by, (c) Gol et al., 2018info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:repositori.udl.cat:10459.1/652092026-06-24T12:42:17Z
dc.title.none.fl_str_mv A polymorphism in the fatty acid desaturase-2 gene is associated with the arachidonic acid metabolism in pigs
title A polymorphism in the fatty acid desaturase-2 gene is associated with the arachidonic acid metabolism in pigs
spellingShingle A polymorphism in the fatty acid desaturase-2 gene is associated with the arachidonic acid metabolism in pigs
Gol, Sofia
Àcids grassos
Porcs
Animals--Cria i desenvolupament
Porcs--Genètica
title_short A polymorphism in the fatty acid desaturase-2 gene is associated with the arachidonic acid metabolism in pigs
title_full A polymorphism in the fatty acid desaturase-2 gene is associated with the arachidonic acid metabolism in pigs
title_fullStr A polymorphism in the fatty acid desaturase-2 gene is associated with the arachidonic acid metabolism in pigs
title_full_unstemmed A polymorphism in the fatty acid desaturase-2 gene is associated with the arachidonic acid metabolism in pigs
title_sort A polymorphism in the fatty acid desaturase-2 gene is associated with the arachidonic acid metabolism in pigs
dc.creator.none.fl_str_mv Gol, Sofia
Pena i Subirà, Ramona Natacha
Rothschild, Max Frederick
Tor i Naudí, Marc
Estany Illa, Joan
author Gol, Sofia
author_facet Gol, Sofia
Pena i Subirà, Ramona Natacha
Rothschild, Max Frederick
Tor i Naudí, Marc
Estany Illa, Joan
author_role author
author2 Pena i Subirà, Ramona Natacha
Rothschild, Max Frederick
Tor i Naudí, Marc
Estany Illa, Joan
author2_role author
author
author
author
dc.subject.none.fl_str_mv Àcids grassos
Porcs
Animals--Cria i desenvolupament
Porcs--Genètica
topic Àcids grassos
Porcs
Animals--Cria i desenvolupament
Porcs--Genètica
description Arachidonic acid (C20:4) is related to a wide range of biological effects including lipid homeostasis. The fatty acid desaturase-2 (FADS2) gene encodes for the delta-6-desaturase, which is involved in the biosynthesis of C20:4 from linoleic acid (C18:2). The purpose of this study was to characterise mutations in the promoter of the porcine FADS2, evaluating in particular the effect of one haplotype tagging polymorphism (rs321384923A > G) on the biosynthesis pathway of C20:4. A total of 1,192 Duroc barrows with records on fatty acid composition in muscle and subcutaneous fat were genotyped. Pigs carrying the A allele showed, irrespective of fat content, both enhanced FADS2 expression and higher C20:4 in muscle and exhibited increased ratios of C20:4 to C18:2 and of C20:4 to eicosadienoic acid (C20:2) in both muscle and adipose tissue. Despite the inverse relationship observed between C20:4 and fat content, the rs321384923 polymorphism had no impact on lean weight. It is concluded that the haplotype encompassing the rs321384923 polymorphism at the porcine FADS2 affects the n-6 fatty acid profile by specifically modifying the desaturation efficiency of C18:2 to C20:4 rather than by concomitant variations in C18:2 following changes in fat content.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1038/s41598-018-32710-w
http://hdl.handle.net/10459.1/65209
url https://doi.org/10.1038/s41598-018-32710-w
http://hdl.handle.net/10459.1/65209
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO//AGL2015-65846-R
info:eu-repo/grantAgreement/MINECO//BES-2014-FPU13%2F04975
Reproducció del document publicat a: https://doi.org/10.1038/s41598-018-32710-w
Scientific Reports, 2018, p. vol. 8, núm. 14336, p.1-9
dc.rights.none.fl_str_mv cc-by, (c) Gol et al., 2018
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by, (c) Gol et al., 2018
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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